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Open data
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Basic information
Entry | Database: PDB / ID: 4zy8 | ||||||
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Title | K. lactis Lst4 longin domain | ||||||
![]() | Protein LST4 | ||||||
![]() | TRANSPORT PROTEIN / longin / DENN | ||||||
Function / homology | ![]() FNIP-folliculin RagC/D GAP / vacuolar membrane / amino acid transport / positive regulation of TORC1 signaling / GTPase activator activity / protein transport Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pacitto, A. / Ascher, D.B. / Blundell, T.L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Lst4, the yeast Fnip1/2 orthologue, is a DENN-family protein. Authors: Pacitto, A. / Ascher, D.B. / Wong, L.H. / Blaszczyk, B.K. / Nookala, R.K. / Zhang, N. / Dokudovskaya, S. / Levine, T.P. / Blundell, T.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 127 KB | Display | ![]() |
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PDB format | ![]() | 97.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 456.2 KB | Display | ![]() |
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Full document | ![]() | 463 KB | Display | |
Data in XML | ![]() | 23.6 KB | Display | |
Data in CIF | ![]() | 32.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20640.516 Da / Num. of mol.: 4 / Fragment: UNP residues 58-226 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.22 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 5.8 / Details: Sodium chloride, Butan-1-ol, Bis-Tris |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Aug 12, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.14→35.29 Å / Num. obs: 38603 / % possible obs: 100 % / Redundancy: 12.1 % / Rmerge(I) obs: 0.066 / Net I/σ(I): 20.4 |
Reflection shell | Resolution: 2.14→2.216 Å / Redundancy: 11.9 % / Rmerge(I) obs: 0.632 / Mean I/σ(I) obs: 4.4 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.14→35.29 Å
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Refine LS restraints |
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LS refinement shell |
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