- PDB-5d92: Structure of a phosphatidylinositolphosphate (PIP) synthase from ... -
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基本情報
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データベース: PDB / ID: 5d92
タイトル
Structure of a phosphatidylinositolphosphate (PIP) synthase from Renibacterium Salmoninarum
要素
AF2299 protein,Phosphatidylinositol synthase
キーワード
MEMBRANE PROTEIN / Enzyme / lipid biosynthesis / phosphatidylinositol
機能・相同性
機能・相同性情報
phosphotransferase activity, for other substituted phosphate groups / 転移酵素; リンを含む基を移すもの; その他の、リン酸基を含む基を移すもの / phospholipid biosynthetic process / nucleotide binding / magnesium ion binding / membrane / metal ion binding / plasma membrane 類似検索 - 分子機能
Component-ID: 1 / Ens-ID: 1 / Beg auth comp-ID: MET / Beg label comp-ID: MET / End auth comp-ID: ALA / End label comp-ID: ALA / Auth seq-ID: -137 - 204 / Label seq-ID: 1 - 342
温度: 293 K / 手法: 脂質キュービック相法 詳細: 30% (v/v) PEG 300, 0.1 M MES pH 6.0, 0.1 M sodium chloride, 0.1 M magnesium chloride (precipitant); Concentrated protein was mixed with molten monoolein in a 1:1.5 (w/w) ratio of protein: ...詳細: 30% (v/v) PEG 300, 0.1 M MES pH 6.0, 0.1 M sodium chloride, 0.1 M magnesium chloride (precipitant); Concentrated protein was mixed with molten monoolein in a 1:1.5 (w/w) ratio of protein:lipid using coupled syringes. A Mosquito LCP (TTP Labtech) robot was used to dispense a typical volume of 50-75 nL of protein/lipid mixture onto a 96-well glass sandwich plate, which was covered with 750 nL precipitant solution. Monoolein was doped with 2% CDP-DAG.