+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 5c9v | |||||||||
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タイトル | Structure of human Parkin G319A | |||||||||
要素 | E3 ubiquitin-protein ligase parkin | |||||||||
キーワード | SIGNALING PROTEIN / Parkin / ubiquitin / E3 ligase / RBR / Parkinson's disease / mitophagy / cell signalling | |||||||||
機能・相同性 | 機能・相同性情報 negative regulation of primary amine oxidase activity / positive regulation of retrograde transport, endosome to Golgi / regulation of lipid transport / positive regulation of neurotransmitter uptake / negative regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway / regulation protein catabolic process at presynapse / regulation of protein targeting to mitochondrion / negative regulation of spontaneous neurotransmitter secretion / negative regulation of intralumenal vesicle formation / negative regulation of glucokinase activity ...negative regulation of primary amine oxidase activity / positive regulation of retrograde transport, endosome to Golgi / regulation of lipid transport / positive regulation of neurotransmitter uptake / negative regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway / regulation protein catabolic process at presynapse / regulation of protein targeting to mitochondrion / negative regulation of spontaneous neurotransmitter secretion / negative regulation of intralumenal vesicle formation / negative regulation of glucokinase activity / negative regulation of exosomal secretion / mitochondrion to lysosome vesicle-mediated transport / type 2 mitophagy / Lewy body / protein K27-linked ubiquitination / Parkin-FBXW7-Cul1 ubiquitin ligase complex / negative regulation of actin filament bundle assembly / positive regulation of mitochondrial fusion / free ubiquitin chain polymerization / regulation of synaptic vesicle transport / negative regulation of mitochondrial fusion / protein K29-linked ubiquitination / : / positive regulation of protein linear polyubiquitination / F-box domain binding / RBR-type E3 ubiquitin transferase / negative regulation by host of viral genome replication / regulation of necroptotic process / positive regulation of mitophagy / regulation of cellular response to oxidative stress / regulation of dopamine metabolic process / dopaminergic synapse / negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator / cellular response to toxic substance / positive regulation of dendrite extension / protein K6-linked ubiquitination / positive regulation of tumor necrosis factor-mediated signaling pathway / positive regulation of proteasomal protein catabolic process / norepinephrine metabolic process / protein localization to mitochondrion / negative regulation of JNK cascade / positive regulation of protein localization to membrane / negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway / cellular response to dopamine / mitochondrial fission / protein K11-linked ubiquitination / aggresome assembly / ubiquitin conjugating enzyme binding / regulation of mitochondrion organization / regulation of canonical Wnt signaling pathway / autophagy of mitochondrion / aggresome / regulation of reactive oxygen species metabolic process / regulation of synaptic vesicle endocytosis / positive regulation of DNA binding / positive regulation of mitochondrial fission / dopamine uptake involved in synaptic transmission / regulation of dopamine secretion / negative regulation of release of cytochrome c from mitochondria / ubiquitin-specific protease binding / dopamine metabolic process / protein deubiquitination / startle response / protein monoubiquitination / cullin family protein binding / phospholipase binding / protein K63-linked ubiquitination / regulation of protein ubiquitination / regulation of glucose metabolic process / mitophagy / cellular response to unfolded protein / negative regulation of reactive oxygen species metabolic process / cellular response to manganese ion / protein autoubiquitination / negative regulation of insulin secretion / proteasomal protein catabolic process / protein K48-linked ubiquitination / negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway / ubiquitin ligase complex / ERAD pathway / heat shock protein binding / response to endoplasmic reticulum stress / Hsp70 protein binding / tubulin binding / negative regulation of protein phosphorylation / adult locomotory behavior / regulation of mitochondrial membrane potential / Josephin domain DUBs / PINK1-PRKN Mediated Mitophagy / learning / ubiquitin binding / mitochondrion organization / regulation of autophagy / central nervous system development / synaptic transmission, glutamatergic / G protein-coupled receptor binding / PDZ domain binding / macroautophagy / protein destabilization / regulation of protein stability 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 2.35 Å | |||||||||
データ登録者 | Wauer, T. / Komander, D. | |||||||||
資金援助 | 英国, 2件
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引用 | ジャーナル: Nature / 年: 2015 タイトル: Mechanism of phospho-ubiquitin-induced PARKIN activation. 著者: Wauer, T. / Simicek, M. / Schubert, A. / Komander, D. #1: ジャーナル: EMBO J. / 年: 2013 タイトル: Structure of the human Parkin ligase domain in an autoinhibited state. 著者: Wauer, T. / Komander, D. | |||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 5c9v.cif.gz | 138.6 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb5c9v.ent.gz | 107 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 5c9v.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 5c9v_validation.pdf.gz | 448 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 5c9v_full_validation.pdf.gz | 449.5 KB | 表示 | |
XML形式データ | 5c9v_validation.xml.gz | 14.1 KB | 表示 | |
CIF形式データ | 5c9v_validation.cif.gz | 18.8 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/c9/5c9v ftp://data.pdbj.org/pub/pdb/validation_reports/c9/5c9v | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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単位格子 |
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Components on special symmetry positions |
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-要素
#1: タンパク質 | 分子量: 36892.129 Da / 分子数: 1 / 断片: UNP residues 137-465 / 変異: G319A / 由来タイプ: 組換発現 / 詳細: engineered mutation at position G319A / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: PARK2, PRKN / プラスミド: pOPINK 発現宿主: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (大腸菌) 参照: UniProt: O60260, 合成酵素; C-N結合を形成; 酸-D-アミノ酸リガーゼ(ペプチド合成) | ||||||
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#2: 化合物 | ChemComp-ZN / #3: 化合物 | ChemComp-SO4 / #4: 化合物 | #5: 水 | ChemComp-HOH / | |
-実験情報
-実験
実験 | 手法: X線回折 |
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-試料調製
結晶 | マシュー密度: 3.63 Å3/Da / 溶媒含有率: 66.1 % |
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結晶化 | 温度: 291 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 5.6 詳細: 1.8 M lithium sulphate, 0.01 M MgCl2, 0.05 M MES pH 5.6 PH範囲: 5.6 |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: シンクロトロン / サイト: Diamond / ビームライン: I04-1 / 波長: 0.9173 Å |
検出器 | タイプ: DECTRIS PILATUS 6M-F / 検出器: PIXEL / 日付: 2015年4月20日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.9173 Å / 相対比: 1 |
反射 | 解像度: 2.35→86.68 Å / Num. obs: 22270 / % possible obs: 100 % / Observed criterion σ(I): 2 / 冗長度: 6.9 % / Biso Wilson estimate: 42.5 Å2 / Rmerge(I) obs: 0.089 / Net I/σ(I): 13.3 |
反射 シェル | 解像度: 2.35→2.43 Å / 冗長度: 6.8 % / Rmerge(I) obs: 0.8 / Mean I/σ(I) obs: 2.1 / % possible all: 100 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: 4bm9 解像度: 2.35→84.68 Å / SU ML: 0.22 / 交差検証法: FREE R-VALUE / σ(F): 1.33 / 位相誤差: 23.8 / 立体化学のターゲット値: ML
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 54.3 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2.35→84.68 Å
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拘束条件 |
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LS精密化 シェル |
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精密化 TLS | 手法: refined / Origin x: 7.8845 Å / Origin y: 31.4194 Å / Origin z: 36.9402 Å
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精密化 TLSグループ | Selection details: chain A |