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Open data
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Basic information
Entry | Database: PDB / ID: 5b7b | ||||||||||||
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Title | Crystal structure of Nucleoprotein-nucleozin complex | ||||||||||||
![]() | Nucleoprotein | ||||||||||||
![]() | VIRAL PROTEIN / influenza nucleoprotein / nucleozin | ||||||||||||
Function / homology | ![]() negative stranded viral RNA replication / helical viral capsid / viral penetration into host nucleus / host cell / viral nucleocapsid / ribonucleoprotein complex / symbiont entry into host cell / host cell nucleus / structural molecule activity / RNA binding / identical protein binding Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Pang, B. / Zhang, W.Z. / Zhang, H.M. / Hao, Q. | ||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Structural Characterization of H1N1 Nucleoprotein-Nucleozin Binding Sites Authors: Pang, B. / Cheung, N.N. / Zhang, W.Z. / Dai, J. / Kao, R.Y. / Zhang, H.M. / Hao, Q. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 491.1 KB | Display | ![]() |
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PDB format | ![]() | 404.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 952.8 KB | Display | ![]() |
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Full document | ![]() | 989.6 KB | Display | |
Data in XML | ![]() | 83.6 KB | Display | |
Data in CIF | ![]() | 111.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2iqhS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Beg auth comp-ID: ASN / Beg label comp-ID: ASN / End auth comp-ID: PHE / End label comp-ID: PHE / Refine code: 3 / Auth seq-ID: 21 - 489 / Label seq-ID: 21 - 489
NCS ensembles :
NCS oper:
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Components
#1: Protein | Mass: 56454.793 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: A/Wilson-Smith/1933 H1N1 / Gene: NP / Production host: ![]() ![]() #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.26 Å3/Da / Density % sol: 45.66 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion / pH: 6 Details: 0.1 M sodium acetate, 0.05 M magnesium acetate, 0.1 M MES, 7% PEG8000 |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Oct 15, 2011 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97916 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 3→50 Å / Num. obs: 55848 / % possible obs: 92.3 % / Redundancy: 3.3 % / Rmerge(I) obs: 0.086 / Net I/av σ(I): 16.094 / Net I/σ(I): 9.7 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2IQH Resolution: 3→50 Å / Cor.coef. Fo:Fc: 0.935 / Cor.coef. Fo:Fc free: 0.893 / SU B: 21.35 / SU ML: 0.374 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.505 / Details: U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 189.43 Å2 / Biso mean: 75.76 Å2 / Biso min: 34.83 Å2
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Refinement step | Cycle: final / Resolution: 3→50 Å
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Refine LS restraints |
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Refine LS restraints NCS | Dom-ID: 1 / Refine-ID: X-RAY DIFFRACTION
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LS refinement shell | Resolution: 3→3.078 Å / Total num. of bins used: 20
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