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Open data
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Basic information
Entry | Database: PDB / ID: 2iqh | ||||||
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Title | Influenza A virus nucleoprotein NP at 3.2A resolution | ||||||
![]() | Nucleocapsid protein | ||||||
![]() | VIRAL PROTEIN / oligomerization / RNA binding / NLS / polymerase binding | ||||||
Function / homology | ![]() helical viral capsid / viral penetration into host nucleus / host cell / viral nucleocapsid / ribonucleoprotein complex / symbiont entry into host cell / host cell nucleus / structural molecule activity / RNA binding / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Ye, Q. / Tao, Y.J. | ||||||
![]() | ![]() Title: The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA. Authors: Ye, Q. / Krug, R.M. / Tao, Y.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 256.4 KB | Display | ![]() |
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PDB format | ![]() | 209.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 463.2 KB | Display | ![]() |
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Full document | ![]() | 529.7 KB | Display | |
Data in XML | ![]() | 53.7 KB | Display | |
Data in CIF | ![]() | 72.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 56806.047 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Genus: Influenzavirus A / Species: Influenza A virus / Strain: A/Wilson-Smith/1933(H1N1) / Gene: NP / Plasmid: pET28a / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.9 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 100mM Tris HCl, 3% PEG 8000, 10% Glycerol, 10mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction |
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Diffraction source |
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 12, 2005 | |||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 3.2→30 Å / Num. obs: 26880 / % possible obs: 99.3 % / Observed criterion σ(I): 13.2 / Redundancy: 4.1 % / Rmerge(I) obs: 0.069 / Net I/σ(I): 13.2 | |||||||||||||||
Reflection shell | Resolution: 3.2→3.31 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.429 / Mean I/σ(I) obs: 3 / % possible all: 97.9 |
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Processing
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Refinement | Method to determine structure: AB INITIO PHASING / Resolution: 3.2→30 Å / Cor.coef. Fo:Fc: 0.885 / Cor.coef. Fo:Fc free: 0.843 / SU B: 30.278 / SU ML: 0.524 / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / ESU R Free: 0.653 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 74.91 Å2
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Refinement step | Cycle: LAST / Resolution: 3.2→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.2→3.283 Å / Total num. of bins used: 20
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