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- PDB-5azt: Ternary complex of hPPARalpha ligand binding domain, 17-oxoDHA an... -

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Basic information

Entry
Database: PDB / ID: 5azt
TitleTernary complex of hPPARalpha ligand binding domain, 17-oxoDHA and a SRC1 peptide
Components
  • 15-meric peptide from Nuclear receptor coactivator 1
  • Peroxisome proliferator-activated receptor alpha
KeywordsDNA BINDING PROTEIN / NUCLEAR RECEPTOR / TRANSCRIPTION REGULATION / LIGAND BINDING DOMAIN / ZINC-FINGER / DNA BINDING / TRANSCRIPTION / OBESITY / NUCLEUS / RECEPTOR / ACTIVATOR / OXIDIZED FATTY ACID / TRANSCRIPTION FACTOR / AGONIST / DUAL AGONIST / COVALENT / PPRE / NUCLEAR / CO-ACTIVATOR
Function / homology
Function and homology information


regulation of fatty acid transport / enamel mineralization / positive regulation of fatty acid beta-oxidation / cellular response to fructose stimulus / regulation of ketone metabolic process / negative regulation of cell growth involved in cardiac muscle cell development / regulation of fatty acid metabolic process / negative regulation of appetite / negative regulation of hepatocyte apoptotic process / positive regulation of fatty acid oxidation ...regulation of fatty acid transport / enamel mineralization / positive regulation of fatty acid beta-oxidation / cellular response to fructose stimulus / regulation of ketone metabolic process / negative regulation of cell growth involved in cardiac muscle cell development / regulation of fatty acid metabolic process / negative regulation of appetite / negative regulation of hepatocyte apoptotic process / positive regulation of fatty acid oxidation / behavioral response to nicotine / lipoprotein metabolic process / negative regulation of leukocyte cell-cell adhesion / labyrinthine layer morphogenesis / regulation of thyroid hormone receptor signaling pathway / positive regulation of transcription from RNA polymerase II promoter by galactose / positive regulation of female receptivity / mitogen-activated protein kinase kinase kinase binding / ubiquitin conjugating enzyme binding / negative regulation of glycolytic process / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / positive regulation of fatty acid metabolic process / NFAT protein binding / DNA-binding transcription activator activity / male mating behavior / hypothalamus development / negative regulation of cholesterol storage / nuclear steroid receptor activity / progesterone receptor signaling pathway / cellular response to Thyroglobulin triiodothyronine / Synthesis of bile acids and bile salts / positive regulation of ATP biosynthetic process / negative regulation of macrophage derived foam cell differentiation / epidermis development / Synthesis of bile acids and bile salts via 27-hydroxycholesterol / Endogenous sterols / Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol / response to retinoic acid / protein-lysine-acetyltransferase activity / positive regulation of lipid biosynthetic process / estrous cycle / nuclear retinoid X receptor binding / phosphatase binding / Recycling of bile acids and salts / Transcriptional regulation of brown and beige adipocyte differentiation by EBF2 / histone acetyltransferase / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / cellular response to hormone stimulus / estrogen receptor signaling pathway / intracellular receptor signaling pathway / nitric oxide metabolic process / negative regulation of reactive oxygen species biosynthetic process / Regulation of lipid metabolism by PPARalpha / negative regulation of blood pressure / lactation / peroxisome proliferator activated receptor signaling pathway / response to progesterone / hormone-mediated signaling pathway / positive regulation of adipose tissue development / regulation of cellular response to insulin stimulus / BMAL1:CLOCK,NPAS2 activates circadian expression / response to nutrient / negative regulation of cytokine production involved in inflammatory response / positive regulation of neuron differentiation / SUMOylation of transcription cofactors / MDM2/MDM4 family protein binding / RORA,B,C and NR1D1 (REV-ERBA) regulate gene expression / Activation of gene expression by SREBF (SREBP) / Expression of BMAL (ARNTL), CLOCK, and NPAS2 / negative regulation of miRNA transcription / positive regulation of gluconeogenesis / cerebellum development / nuclear estrogen receptor binding / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / nuclear receptor binding / cellular response to starvation / gluconeogenesis / RNA polymerase II transcription regulatory region sequence-specific DNA binding / SUMOylation of intracellular receptors / hippocampus development / circadian regulation of gene expression / wound healing / Heme signaling / negative regulation of transforming growth factor beta receptor signaling pathway / PPARA activates gene expression / Transcriptional activation of mitochondrial biogenesis / Cytoprotection by HMOX1 / fatty acid metabolic process / Nuclear Receptor transcription pathway / Transcriptional regulation of white adipocyte differentiation / regulation of circadian rhythm / response to insulin / cerebral cortex development / mRNA transcription by RNA polymerase II / DNA-binding transcription repressor activity, RNA polymerase II-specific / male gonad development / nuclear receptor activity / negative regulation of inflammatory response / transcription coactivator binding / RNA polymerase II transcription regulator complex
Similarity search - Function
Peroxisome proliferator-activated receptor alpha / Nuclear receptor coactivator 1 / Peroxisome proliferator-activated receptor / Nuclear receptor coactivator, DUF1518 / Nuclear receptor coactivator, Ncoa-type, interlocking / Nuclear receptor coactivator, Ncoa-type, interlocking domain superfamily / Nuclear receptor coactivator, DUF1518 / Nuclear receptor coactivator / DUF1518 / Nuclear receptor coactivator, receptor-binding domain ...Peroxisome proliferator-activated receptor alpha / Nuclear receptor coactivator 1 / Peroxisome proliferator-activated receptor / Nuclear receptor coactivator, DUF1518 / Nuclear receptor coactivator, Ncoa-type, interlocking / Nuclear receptor coactivator, Ncoa-type, interlocking domain superfamily / Nuclear receptor coactivator, DUF1518 / Nuclear receptor coactivator / DUF1518 / Nuclear receptor coactivator, receptor-binding domain / Nuclear receptor coactivator / : / Steroid receptor coactivator / Unstructured region on nuclear receptor coactivator protein / Nuclear receptor coactivators bHLH domain / PAS domain / Nuclear receptor coactivator, interlocking / helix loop helix domain / Myc-type, basic helix-loop-helix (bHLH) domain / Myc-type, basic helix-loop-helix (bHLH) domain profile. / Helix-loop-helix DNA-binding domain superfamily / : / PAS fold / PAS fold / PAS domain / PAS repeat profile. / PAS domain / Retinoid X Receptor / Retinoid X Receptor / PAS domain superfamily / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Double treble clef zinc finger, C4 type / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors / Nuclear hormone receptor, ligand-binding domain / Nuclear hormone receptor-like domain superfamily / Ligand-binding domain of nuclear hormone receptor / Nuclear receptor (NR) ligand-binding (LBD) domain profile. / Ligand binding domain of hormone receptors / Zinc finger, NHR/GATA-type / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Chem-4M5 / Peroxisome proliferator-activated receptor alpha / Nuclear receptor coactivator 1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3.45 Å
AuthorsEgawa, D. / Itoh, T. / Yamamoto, K.
CitationJournal: Acs Chem.Biol. / Year: 2016
Title: 17-OxoDHA Is a PPAR alpha/gamma Dual Covalent Modifier and Agonist
Authors: Egawa, D. / Itoh, T. / Akiyama, Y. / Saito, T. / Yamamoto, K.
History
DepositionOct 23, 2015Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jul 6, 2016Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2016Group: Database references
Revision 1.2Feb 26, 2020Group: Data collection / Derived calculations / Category: diffrn_source / pdbx_struct_oper_list
Item: _diffrn_source.pdbx_synchrotron_site / _pdbx_struct_oper_list.symmetry_operation
Revision 1.3Oct 30, 2024Group: Data collection / Database references / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Peroxisome proliferator-activated receptor alpha
B: Peroxisome proliferator-activated receptor alpha
C: 15-meric peptide from Nuclear receptor coactivator 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)63,2665
Polymers62,5773
Non-polymers6892
Water43224
1
A: Peroxisome proliferator-activated receptor alpha
C: 15-meric peptide from Nuclear receptor coactivator 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)32,5573
Polymers32,2132
Non-polymers3441
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1010 Å2
ΔGint-9 kcal/mol
Surface area12770 Å2
MethodPISA
2
B: Peroxisome proliferator-activated receptor alpha
hetero molecules


Theoretical massNumber of molelcules
Total (without water)30,7092
Polymers30,3651
Non-polymers3441
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area0 Å2
ΔGint0 kcal/mol
Surface area12010 Å2
MethodPISA
Unit cell
Length a, b, c (Å)59.460, 59.460, 317.940
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number92
Space group name H-MP41212

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Components

#1: Protein Peroxisome proliferator-activated receptor alpha / PPAR-alpha / Nuclear receptor subfamily 1 group C member 1


Mass: 30364.516 Da / Num. of mol.: 2 / Fragment: UNP residues 201-468 / Mutation: F423L
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: PPARA, NR1C1, PPAR / Production host: Escherichia coli (E. coli) / References: UniProt: Q07869
#2: Protein/peptide 15-meric peptide from Nuclear receptor coactivator 1


Mass: 1848.177 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: Q15788
#3: Chemical ChemComp-4M5 / (4~{Z},7~{Z},10~{Z},13~{Z},19~{Z})-17-oxidanylidenedocosa-4,7,10,13,19-pentaenoic acid


Mass: 344.488 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C22H32O3
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 24 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.25 Å3/Da / Density % sol: 45.22 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.9 / Details: 0.1M HEPES, 35% PEG3350

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NW12A / Wavelength: 1 Å
DetectorType: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 26, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3.45→47.61 Å / Num. obs: 8185 / % possible obs: 98.5 % / Redundancy: 10.5 % / Net I/σ(I): 15.6

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Processing

SoftwareName: REFMAC / Version: 5.8.0103 / Classification: refinement
RefinementResolution: 3.45→11 Å / Cor.coef. Fo:Fc: 0.853 / Cor.coef. Fo:Fc free: 0.74 / SU B: 40.739 / SU ML: 0.65 / Cross valid method: THROUGHOUT / ESU R Free: 0.823 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.30763 400 5.1 %RANDOM
Rwork0.25254 ---
obs0.25537 7408 94.46 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 31.093 Å2
Baniso -1Baniso -2Baniso -3
1-0.51 Å2-0 Å2-0 Å2
2--0.51 Å2-0 Å2
3----1.03 Å2
Refinement stepCycle: 1 / Resolution: 3.45→11 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4046 0 50 24 4120
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0070.0194182
X-RAY DIFFRACTIONr_bond_other_d0.0030.024101
X-RAY DIFFRACTIONr_angle_refined_deg1.2571.9845644
X-RAY DIFFRACTIONr_angle_other_deg2.12439412
X-RAY DIFFRACTIONr_dihedral_angle_1_deg4.7775521
X-RAY DIFFRACTIONr_dihedral_angle_2_deg39.65124.943174
X-RAY DIFFRACTIONr_dihedral_angle_3_deg19.14215736
X-RAY DIFFRACTIONr_dihedral_angle_4_deg20.951516
X-RAY DIFFRACTIONr_chiral_restr0.0710.2664
X-RAY DIFFRACTIONr_gen_planes_refined0.0050.024674
X-RAY DIFFRACTIONr_gen_planes_other0.0020.02920
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it1.2173.1642096
X-RAY DIFFRACTIONr_mcbond_other1.2153.1632095
X-RAY DIFFRACTIONr_mcangle_it2.1054.7352613
X-RAY DIFFRACTIONr_mcangle_other2.1044.7372614
X-RAY DIFFRACTIONr_scbond_it1.5643.2222086
X-RAY DIFFRACTIONr_scbond_other1.5623.222084
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other2.3594.8063031
X-RAY DIFFRACTIONr_long_range_B_refined4.27625.0335065
X-RAY DIFFRACTIONr_long_range_B_other4.27625.0395066
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 3.45→3.531 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.282 18 -
Rwork0.27 476 -
obs--90.81 %

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