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- PDB-5azo: Crystal structure of a membrane protein from Pseudomonas aeruginosa -
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Open data
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Basic information
Entry | Database: PDB / ID: 5azo | ||||||
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Title | Crystal structure of a membrane protein from Pseudomonas aeruginosa | ||||||
![]() | Multidrug efflux outer membrane protein OprN | ||||||
![]() | MEMBRANE PROTEIN / Alpha barrel / Beta barrel | ||||||
Function / homology | ![]() efflux transmembrane transporter activity / transmembrane transporter activity / cell outer membrane / transmembrane transport / membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yonehara, R. / Yamashita, E. / Nakagawa, A. | ||||||
![]() | ![]() Title: Crystal structures of OprN and OprJ, outer membrane factors of multidrug tripartite efflux pumps of Pseudomonas aeruginosa. Authors: Yonehara, R. / Yamashita, E. / Nakagawa, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 177.2 KB | Display | ![]() |
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PDB format | ![]() | 141.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 423.3 KB | Display | ![]() |
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Full document | ![]() | 423.4 KB | Display | |
Data in XML | ![]() | 15.7 KB | Display | |
Data in CIF | ![]() | 20.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5azpC ![]() 5azsC ![]() 1wp1S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 49686.188 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 26-472 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228 / Gene: oprN, PA2495 / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.26 Å3/Da / Density % sol: 62.29 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5 / Details: MPD, sodium acetate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: Dec 17, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→50 Å / Num. obs: 18601 / % possible obs: 100 % / Redundancy: 10.9 % / Net I/σ(I): 17.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1WP1 Resolution: 2.7→50 Å / Cor.coef. Fo:Fc: 0.924 / Cor.coef. Fo:Fc free: 0.88 / SU B: 26.869 / SU ML: 0.239 / Cross valid method: THROUGHOUT / ESU R: 0.581 / ESU R Free: 0.327 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 49.44 Å2
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Refinement step | Cycle: 1 / Resolution: 2.7→50 Å
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Refine LS restraints |
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