- PDB-5aie: Not4 ring domain in complex with Ubc4 -
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Basic information
Entry
Database: PDB / ID: 5aie
Title
Not4 ring domain in complex with Ubc4
Components
GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 4
UBIQUITIN-CONJUGATING ENZYME E2 4
Keywords
LIGASE / SIGNALING PROTEIN / NOT4 RING DOMAIN / UBC4 / E2-E3 LIGASE
Function / homology
Function and homology information
CCR4-NOT core complex / deadenylation-independent decapping of nuclear-transcribed mRNA / CCR4-NOT complex / Peroxisomal protein import / cytoplasm protein quality control by the ubiquitin-proteasome system / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / mitochondria-associated ubiquitin-dependent protein catabolic process / nuclear-transcribed mRNA poly(A) tail shortening / E3 ubiquitin ligases ubiquitinate target proteins / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway ...CCR4-NOT core complex / deadenylation-independent decapping of nuclear-transcribed mRNA / CCR4-NOT complex / Peroxisomal protein import / cytoplasm protein quality control by the ubiquitin-proteasome system / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / mitochondria-associated ubiquitin-dependent protein catabolic process / nuclear-transcribed mRNA poly(A) tail shortening / E3 ubiquitin ligases ubiquitinate target proteins / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / ribosome-associated ubiquitin-dependent protein catabolic process / E2 ubiquitin-conjugating enzyme / proteasome binding / protein monoubiquitination / ubiquitin conjugating enzyme activity / Antigen processing: Ubiquitination & Proteasome degradation / proteasomal protein catabolic process / protein K48-linked ubiquitination / ubiquitin ligase complex / rescue of stalled ribosome / ubiquitin binding / positive regulation of transcription elongation by RNA polymerase II / RING-type E3 ubiquitin transferase / protein polyubiquitination / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / protein-macromolecule adaptor activity / ubiquitin-dependent protein catabolic process / protein ubiquitination / ubiquitin protein ligase binding / RNA binding / ATP binding / nucleus / metal ion binding / cytoplasm Similarity search - Function
Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
Sequence details
RSM RESIDUES ARE LEFT OVER AFTER THE TAG CLEAVAGE TGSTGSTETG RESIDUES ARE THE ARTIFICIAL LINKER ...RSM RESIDUES ARE LEFT OVER AFTER THE TAG CLEAVAGE TGSTGSTETG RESIDUES ARE THE ARTIFICIAL LINKER USED TO FUSE THE C-TERMINUS OF THE RING DOMAIN OF NOT4 WITH THE N- TERMINAL METHIONINE OF THE UBC4
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.85 Å3/Da / Density % sol: 56.86 % / Description: NONE
Crystal grow
pH: 8.5 Details: 10% (W/V) PEG 8000, 0.02 M OF L-NA-GLUTAMATE, 0.02 M OF ALANINE (RACEMIC), 0.02 M OF GLYCINE, 0.02 M OF LYSINE HCL (RACEMIC), 0.02 M OF SERINE (RACEMIC), 0.1 M BICINE PH 8.5 AND 20% (W/V) ...Details: 10% (W/V) PEG 8000, 0.02 M OF L-NA-GLUTAMATE, 0.02 M OF ALANINE (RACEMIC), 0.02 M OF GLYCINE, 0.02 M OF LYSINE HCL (RACEMIC), 0.02 M OF SERINE (RACEMIC), 0.1 M BICINE PH 8.5 AND 20% (W/V) ETHYLENE GLYCOL AS CRYSTALLIZATION BUFFER AT ROOM TEMPERATURE.
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