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- PDB-5aa4: Crystal structure of MltF from Pseudomonas aeruginosa in complex ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5aa4 | ||||||
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Title | Crystal structure of MltF from Pseudomonas aeruginosa in complex with cell-wall tetrapeptide | ||||||
![]() | (MEMBRANE-BOUND LYTIC MUREIN TRANSGLYCOSYLASE F) x 2 | ||||||
![]() | LYASE / LYTIC TRANSGLYCOSILASE / CELL WALL RECYCLING | ||||||
Function / homology | ![]() carbon-oxygen lyase activity, acting on polysaccharides / : / lytic transglycosylase activity / peptidoglycan metabolic process / cell outer membrane / cell wall organization / cell wall macromolecule catabolic process Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Dominguez-Gil, T. / Acebron, I. / Hermoso, J.A. | ||||||
![]() | ![]() Title: Activation by Allostery in Cell-Wall Remodeling by a Modular Membrane-Bound Lytic Transglycosylase from Pseudomonas aeruginosa. Authors: Dominguez-Gil, T. / Lee, M. / Acebron-Avalos, I. / Mahasenan, K.V. / Hesek, D. / Dik, D.A. / Byun, B. / Lastochkin, E. / Fisher, J.F. / Mobashery, S. / Hermoso, J.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 619.4 KB | Display | ![]() |
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PDB format | ![]() | 518.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 745.4 KB | Display | ![]() |
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Full document | ![]() | 772.1 KB | Display | |
Data in XML | ![]() | 59.9 KB | Display | |
Data in CIF | ![]() | 83.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5a5xSC ![]() 5aa1C ![]() 5aa2C ![]() 5aa3C C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 51113.395 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: A0A077JMS0, UniProt: A0A1I9GEN8*PLUS, Lyases; Carbon-oxygen lyases; Acting on polysaccharides #2: Protein | Mass: 51143.418 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: A0A077JMS0, UniProt: A0A1I9GEN9*PLUS, Lyases; Carbon-oxygen lyases; Acting on polysaccharides #3: Chemical | ChemComp-6X4 / [ | #4: Water | ChemComp-HOH / | Sequence details | FIRST NINE AMINOACIDS | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.94 Å3/Da / Density % sol: 58.15 % / Description: NONE |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Details: ELLIPTICALLY BENT MIRROR |
Radiation | Monochromator: SI(111) CHANNEL-CUT, CRYOCOOLED / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→48.72 Å / Num. obs: 94338 / % possible obs: 98.5 % / Observed criterion σ(I): 0 / Redundancy: 6.7 % / Rmerge(I) obs: 0.09 / Net I/σ(I): 13.8 |
Reflection shell | Resolution: 2.4→2.44 Å / Redundancy: 6.7 % / Rmerge(I) obs: 1.04 / Mean I/σ(I) obs: 1.7 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 5A5X Resolution: 2.4→48.569 Å / SU ML: 0.35 / σ(F): 1.33 / Phase error: 26.73 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→48.569 Å
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Refine LS restraints |
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LS refinement shell |
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