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Open data
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Basic information
Entry | Database: PDB / ID: 4zvc | ||||||
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Title | Crystal structure of MID domain of the E. coli DosC - form I | ||||||
![]() | Diguanylate cyclase DosC | ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() negative regulation of bacterial-type flagellum-dependent cell motility / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tarnawski, M. / Barends, T.R.M. / Schlichting, I. | ||||||
![]() | ![]() Title: Structural analysis of an oxygen-regulated diguanylate cyclase. Authors: Tarnawski, M. / Barends, T.R. / Schlichting, I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 113.9 KB | Display | ![]() |
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PDB format | ![]() | 87.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4zvaC ![]() 4zvbC ![]() 4zvdC ![]() 4zveC ![]() 4zvfC ![]() 4zvgC ![]() 4zvhC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14678.621 Da / Num. of mol.: 2 / Fragment: UNP residues 173-298 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-BEZ / | ![]() #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.88 Å3/Da / Density % sol: 34.59 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion Details: 0.1M Bis-Tris pH 5.5, 0.2M magnesium chloride, 17% (w/v) PEG 3350, 0.1M Gd-HPDO3A [gadolinium complex of 10-(2-hydroxypropyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid] |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 25, 2014 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.5→50 Å / Num. obs: 35038 / % possible obs: 99.7 % / Redundancy: 6.5 % / Rmerge(I) obs: 0.059 / Net I/σ(I): 18.57 |
Reflection shell | Resolution: 1.5→1.6 Å / Redundancy: 6.3 % / Rmerge(I) obs: 0.38 / Mean I/σ(I) obs: 5.37 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→48.325 Å
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Refine LS restraints |
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LS refinement shell |
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