positive regulation of delayed rectifier potassium channel activity / ROBO receptors bind AKAP5 / regulation of membrane repolarization / cAMP-dependent protein kinase regulator activity / negative regulation of cAMP-dependent protein kinase activity / positive regulation of potassium ion transmembrane transport / nucleotide-activated protein kinase complex / regulation of protein kinase A signaling / cAMP-dependent protein kinase inhibitor activity / protein kinase A binding ...positive regulation of delayed rectifier potassium channel activity / ROBO receptors bind AKAP5 / regulation of membrane repolarization / cAMP-dependent protein kinase regulator activity / negative regulation of cAMP-dependent protein kinase activity / positive regulation of potassium ion transmembrane transport / nucleotide-activated protein kinase complex / regulation of protein kinase A signaling / cAMP-dependent protein kinase inhibitor activity / protein kinase A binding / CREB1 phosphorylation through the activation of Adenylate Cyclase / PKA activation / ciliary base / action potential / cAMP-dependent protein kinase complex / protein kinase A regulatory subunit binding / plasma membrane raft / protein kinase A catalytic subunit binding / PKA activation in glucagon signalling / DARPP-32 events / lateral plasma membrane / Hedgehog 'off' state / monoatomic ion transport / cAMP binding / cellular response to cAMP / hippocampal mossy fiber to CA3 synapse / FCGR3A-mediated IL10 synthesis / modulation of chemical synaptic transmission / protein localization / Vasopressin regulates renal water homeostasis via Aquaporins / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADORA2B mediated anti-inflammatory cytokines production / GPER1 signaling / Factors involved in megakaryocyte development and platelet production / intracellular signal transduction / apical plasma membrane / protein domain specific binding / focal adhesion / nucleotide binding / centrosome / ubiquitin protein ligase binding / protein kinase binding / protein-containing complex / extracellular exosome / membrane / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能
A-kinase anchor protein 7, RI-RII subunit-binding domain / PKA-RI-RII subunit binding domain of A-kinase anchor protein / Protein kinase A anchor protein, nuclear localisation signal domain / AKAP7 2'5' RNA ligase-like domain / cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / cAMP-dependent Protein Kinase, Chain A / cAMP-dependent protein kinase regulatory subunit / cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / Regulatory subunit of type II PKA R-subunit / RIIalpha, Regulatory subunit portion of type II PKA R-subunit ...A-kinase anchor protein 7, RI-RII subunit-binding domain / PKA-RI-RII subunit binding domain of A-kinase anchor protein / Protein kinase A anchor protein, nuclear localisation signal domain / AKAP7 2'5' RNA ligase-like domain / cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / cAMP-dependent Protein Kinase, Chain A / cAMP-dependent protein kinase regulatory subunit / cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / Regulatory subunit of type II PKA R-subunit / RIIalpha, Regulatory subunit portion of type II PKA R-subunit / Cyclic phosphodiesterase / Cyclic nucleotide-binding domain signature 2. / Cyclic nucleotide-binding domain signature 1. / Cyclic nucleotide-binding, conserved site / Cyclic nucleotide-monophosphate binding domain / Cyclic nucleotide-binding domain / cAMP/cGMP binding motif profile. / Cyclic nucleotide-binding domain / Cyclic nucleotide-binding domain superfamily / RmlC-like jelly roll fold / Up-down Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
: / A-kinase anchor protein 7 isoforms alpha and beta / cAMP-dependent protein kinase type II-alpha regulatory subunit / A-kinase anchor protein 7 isoform gamma 類似検索 - 構成要素
A: cAMP-dependent protein kinase type II-alpha regulatory subunit B: cAMP-dependent protein kinase type II-alpha regulatory subunit C: cAMP-dependent protein kinase type II-alpha regulatory subunit D: cAMP-dependent protein kinase type II-alpha regulatory subunit E: cAMP-dependent protein kinase type II-alpha regulatory subunit F: cAMP-dependent protein kinase type II-alpha regulatory subunit G: cAMP-dependent protein kinase type II-alpha regulatory subunit H: cAMP-dependent protein kinase type II-alpha regulatory subunit I: cAMP-dependent protein kinase type II-alpha regulatory subunit J: cAMP-dependent protein kinase type II-alpha regulatory subunit K: cAMP-dependent protein kinase type II-alpha regulatory subunit L: cAMP-dependent protein kinase type II-alpha regulatory subunit M: A-kinase anchor protein 7 isoforms alpha and beta N: A-kinase anchor protein 7 isoforms alpha and beta O: A-kinase anchor protein 7 isoforms alpha and beta P: A-kinase anchor protein 7 isoforms alpha and beta Q: A-kinase anchor protein 7 isoforms alpha and beta R: A-kinase anchor protein 7 isoforms alpha and beta ヘテロ分子
A: cAMP-dependent protein kinase type II-alpha regulatory subunit B: cAMP-dependent protein kinase type II-alpha regulatory subunit M: A-kinase anchor protein 7 isoforms alpha and beta ヘテロ分子
C: cAMP-dependent protein kinase type II-alpha regulatory subunit D: cAMP-dependent protein kinase type II-alpha regulatory subunit N: A-kinase anchor protein 7 isoforms alpha and beta
E: cAMP-dependent protein kinase type II-alpha regulatory subunit F: cAMP-dependent protein kinase type II-alpha regulatory subunit O: A-kinase anchor protein 7 isoforms alpha and beta ヘテロ分子
G: cAMP-dependent protein kinase type II-alpha regulatory subunit H: cAMP-dependent protein kinase type II-alpha regulatory subunit P: A-kinase anchor protein 7 isoforms alpha and beta ヘテロ分子
I: cAMP-dependent protein kinase type II-alpha regulatory subunit J: cAMP-dependent protein kinase type II-alpha regulatory subunit Q: A-kinase anchor protein 7 isoforms alpha and beta
K: cAMP-dependent protein kinase type II-alpha regulatory subunit L: cAMP-dependent protein kinase type II-alpha regulatory subunit R: A-kinase anchor protein 7 isoforms alpha and beta
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.91841 Å / 相対比: 1
Reflection twin
Crystal-ID
ID
Operator
Domain-ID
Fraction
1
1
H, K, L
1
0.914
1
1
L, K, -H
2
0.086
反射
解像度: 2.63→41.5 Å / Num. obs: 22903 / % possible obs: 99.4 % / 冗長度: 3.25 % / Net I/σ(I): 10.61
-
解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.7.0032
精密化
XDS
データ削減
XDS
データスケーリング
PHASER
位相決定
精密化
解像度: 2.63→41.5 Å / Cor.coef. Fo:Fc: 0.906 / Cor.coef. Fo:Fc free: 0.872 / SU B: 10.616 / SU ML: 0.238 / 交差検証法: THROUGHOUT / ESU R: 0.861 / ESU R Free: 0.076 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.2677
1142
5 %
RANDOM
Rwork
0.22233
-
-
-
obs
0.22452
21760
85.62 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK