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Open data
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Basic information
Entry | Database: PDB / ID: 4xlx | ||||||
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Title | Crystal structure of BjKS from Bradyrhizobium japonicum | ||||||
![]() | Uncharacterized protein blr2150 | ||||||
![]() | HYDROLASE / Diterpene synthases | ||||||
Function / homology | Terpene synthase family 2, C-terminal metal binding / Isoprenoid synthase domain superfamily / Uncharacterized protein blr2150![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hu, Y. / Zheng, Y. / Ko, T.P. / Liu, W. / Guo, R.T. | ||||||
![]() | ![]() Title: Structure, function and inhibition of ent-kaurene synthase from Bradyrhizobium japonicum. Authors: Liu, W. / Feng, X. / Zheng, Y. / Huang, C.H. / Nakano, C. / Hoshino, T. / Bogue, S. / Ko, T.P. / Chen, C.C. / Cui, Y. / Li, J. / Wang, I. / Hsu, S.T. / Oldfield, E. / Guo, R.T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 235.9 KB | Display | ![]() |
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PDB format | ![]() | 190.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 456.9 KB | Display | ![]() |
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Full document | ![]() | 481.6 KB | Display | |
Data in XML | ![]() | 48.5 KB | Display | |
Data in CIF | ![]() | 70 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4w4rC ![]() 4w4sC ![]() 4xlyC ![]() 3w3f ![]() 3wbv C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33417.996 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110 / Gene: blr2150 / Plasmid: pET32 Xa/LIC / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.09 Å3/Da / Density % sol: 41.16 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 1.1M ammonium tartrate dibasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 3, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→25 Å / Num. obs: 72234 / % possible obs: 98 % / Redundancy: 3 % / Rmerge(I) obs: 0.056 / Net I/σ(I): 18.1 |
Reflection shell | Resolution: 2→2.1 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.207 / Mean I/σ(I) obs: 3.1 / Num. unique all: 6728 / % possible all: 91.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3W3F ![]() 3w3f Resolution: 2→25 Å / Cross valid method: FREE R-VALUE / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2→25 Å
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LS refinement shell | Resolution: 2→2.1 Å /
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