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- PDB-4wim: Crystal Structure of the GMP Synthetase from Plasmodium falciparum -
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Open data
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Basic information
Entry | Database: PDB / ID: 4wim | |||||||||
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Title | Crystal Structure of the GMP Synthetase from Plasmodium falciparum | |||||||||
![]() | GMP synthetase | |||||||||
![]() | LYASE / GMP synthetase / purine salvage pathway | |||||||||
Function / homology | ![]() Purine ribonucleoside monophosphate biosynthesis / GMP synthase (glutamine-hydrolyzing) activity / Azathioprine ADME / GMP synthase activity / GMP synthase (glutamine-hydrolysing) / GMP biosynthetic process / purine nucleotide biosynthetic process / glutamine metabolic process / magnesium ion binding / ATP binding / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Ballut, L. / Violot, S. / Haser, R. / Aghajari, N. | |||||||||
![]() | ![]() Title: Active site coupling in Plasmodium falciparum GMP synthetase is triggered by domain rotation. Authors: Ballut, L. / Violot, S. / Shivakumaraswamy, S. / Thota, L.P. / Sathya, M. / Kunala, J. / Dijkstra, B.W. / Terreux, R. / Haser, R. / Balaram, H. / Aghajari, N. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 204.1 KB | Display | ![]() |
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PDB format | ![]() | 154.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 456.5 KB | Display | ![]() |
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Full document | ![]() | 636 KB | Display | |
Data in XML | ![]() | 62.4 KB | Display | |
Data in CIF | ![]() | 82.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4winC ![]() 4wioC ![]() 3uowS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 65487.609 Da / Num. of mol.: 2 / Fragment: UNP residues 2-554 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q8IJR9, GMP synthase (glutamine-hydrolysing) #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 20% (w/v) PEG 3350, 0.2 M Diammonium tartrate and 5 mM NaCl |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 6, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97239 Å / Relative weight: 1 |
Reflection | Resolution: 3.6→98.6 Å / Num. obs: 13123 / % possible obs: 88.8 % / Redundancy: 1.7 % / Net I/σ(I): 7.2 |
Reflection shell | Resolution: 3.6→3.96 Å / Redundancy: 1.7 % / Rmerge(I) obs: 0.64 / Mean I/σ(I) obs: 1.34 / % possible all: 84 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3UOW Resolution: 3.6→49.305 Å / SU ML: 0.35 / Cross valid method: FREE R-VALUE / σ(F): 1.99 / Phase error: 37.88 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.6→49.305 Å
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Refine LS restraints |
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LS refinement shell |
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