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- PDB-4w8f: Crystal structure of the dynein motor domain in the AMPPNP-bound state -

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Basic information

Entry
Database: PDB / ID: 4w8f
TitleCrystal structure of the dynein motor domain in the AMPPNP-bound state
ComponentsDynein heavy chain lysozyme chimera
KeywordsMOTOR PROTEIN / cytoplasmic dynein / microtubule / ATPase / AAA+ / AMPPNP
Function / homology
Function and homology information


karyogamy / nuclear migration along microtubule / astral microtubule / establishment of mitotic spindle localization / spindle pole body / minus-end-directed microtubule motor activity / cytoplasmic dynein complex / dynein light intermediate chain binding / nuclear migration / dynein intermediate chain binding ...karyogamy / nuclear migration along microtubule / astral microtubule / establishment of mitotic spindle localization / spindle pole body / minus-end-directed microtubule motor activity / cytoplasmic dynein complex / dynein light intermediate chain binding / nuclear migration / dynein intermediate chain binding / establishment of mitotic spindle orientation / mitotic sister chromatid segregation / viral release from host cell by cytolysis / cytoplasmic microtubule / cytoplasmic microtubule organization / peptidoglycan catabolic process / Neutrophil degranulation / mitotic spindle organization / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / cell cortex / host cell cytoplasm / defense response to bacterium / ATP hydrolysis activity / ATP binding / cytoplasm
Similarity search - Function
Region D6 of dynein motor / Dynein motor heavy chain, linker domain, N-terminal subdomain / AAA+ lid domain / : / DYN1, AAA+ ATPase lid domain / : / Dynein heavy chain, ATPase lid domain / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / P-loop containing dynein motor region ...Region D6 of dynein motor / Dynein motor heavy chain, linker domain, N-terminal subdomain / AAA+ lid domain / : / DYN1, AAA+ ATPase lid domain / : / Dynein heavy chain, ATPase lid domain / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / P-loop containing dynein motor region / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Butyryl-CoA Dehydrogenase, subunit A; domain 3 / Endolysin T4 type / T4-type lysozyme / : / Glycoside hydrolase, family 24 / Phage lysozyme / Lysozyme domain superfamily / Helicase, Ruva Protein; domain 3 / Lysozyme-like domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Up-down Bundle / P-loop containing nucleoside triphosphate hydrolase / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / Endolysin / Dynein heavy chain, cytoplasmic
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
Enterobacteria phage T4 (virus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3.541 Å
AuthorsCheng, H.-C. / Bhabha, G. / Zhang, N. / Vale, R.D.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM09731 United States
CitationJournal: Cell / Year: 2014
Title: Allosteric communication in the dynein motor domain.
Authors: Gira Bhabha / Hui-Chun Cheng / Nan Zhang / Arne Moeller / Maofu Liao / Jeffrey A Speir / Yifan Cheng / Ronald D Vale /
Abstract: Dyneins power microtubule motility using ring-shaped, AAA-containing motor domains. Here, we report X-ray and electron microscopy (EM) structures of yeast dynein bound to different ATP analogs, which ...Dyneins power microtubule motility using ring-shaped, AAA-containing motor domains. Here, we report X-ray and electron microscopy (EM) structures of yeast dynein bound to different ATP analogs, which collectively provide insight into the roles of dynein's two major ATPase sites, AAA1 and AAA3, in the conformational change mechanism. ATP binding to AAA1 triggers a cascade of conformational changes that propagate to all six AAA domains and cause a large movement of the "linker," dynein's mechanical element. In contrast to the role of AAA1 in driving motility, nucleotide transitions in AAA3 gate the transmission of conformational changes between AAA1 and the linker, suggesting that AAA3 acts as a regulatory switch. Further structural and mutational studies also uncover a role for the linker in regulating the catalytic cycle of AAA1. Together, these results reveal how dynein's two major ATP-binding sites initiate and modulate conformational changes in the motor domain during motility.
History
DepositionAug 24, 2014Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 12, 2014Provider: repository / Type: Initial release
Revision 1.1Dec 10, 2014Group: Database references
Revision 1.2Sep 20, 2017Group: Author supporting evidence / Derived calculations ...Author supporting evidence / Derived calculations / Other / Source and taxonomy / Structure summary
Category: entity_src_gen / pdbx_audit_support ...entity_src_gen / pdbx_audit_support / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_assembly_gen / pdbx_struct_assembly_prop / pdbx_struct_oper_list / struct_keywords
Item: _entity_src_gen.pdbx_alt_source_flag / _pdbx_audit_support.funding_organization ..._entity_src_gen.pdbx_alt_source_flag / _pdbx_audit_support.funding_organization / _pdbx_database_status.pdb_format_compatible / _pdbx_struct_assembly.oligomeric_details / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_assembly_prop.type / _pdbx_struct_assembly_prop.value / _pdbx_struct_oper_list.symmetry_operation / _struct_keywords.text
Revision 1.3Dec 25, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.4Dec 27, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / refine_hist / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_label_asym_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Dynein heavy chain lysozyme chimera
B: Dynein heavy chain lysozyme chimera
hetero molecules


Theoretical massNumber of molelcules
Total (without water)613,87812
Polymers609,7802
Non-polymers4,09810
Water00
1
A: Dynein heavy chain lysozyme chimera
hetero molecules


Theoretical massNumber of molelcules
Total (without water)306,9396
Polymers304,8901
Non-polymers2,0495
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Dynein heavy chain lysozyme chimera
hetero molecules


Theoretical massNumber of molelcules
Total (without water)306,9396
Polymers304,8901
Non-polymers2,0495
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3


  • Idetical with deposited unit
  • defined by software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8970 Å2
ΔGint-26 kcal/mol
Surface area227290 Å2
MethodPISA
Unit cell
Length a, b, c (Å)137.730, 154.380, 177.550
Angle α, β, γ (deg.)90.00, 96.59, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein Dynein heavy chain lysozyme chimera / Dynein heavy chain / cytosolic / DYHC / Lysis protein / Muramidase


Mass: 304889.875 Da / Num. of mol.: 2 / Mutation: E1849Q
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast), (gene. exp.) Enterobacteria phage T4 (virus)
Strain: ATCC 204508 / S288c / Gene: DYN1, DHC1, YKR054C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P36022, UniProt: P00720, lysozyme
#2: Chemical
ChemComp-ANP / PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER


Mass: 506.196 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C10H17N6O12P3 / Comment: AMP-PNP, energy-carrying molecule analogue*YM
#3: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 3.13 Å3/Da / Density % sol: 60.7 % / Description: thin plates
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / Details: 4-10% PEG 3350 and 200-300 mM NaAc

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1.1159 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 6, 2012
RadiationMonochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.1159 Å / Relative weight: 1
ReflectionResolution: 3.54→49.4 Å / Num. obs: 82983 / % possible obs: 92.2 % / Observed criterion σ(I): 3.3 / Redundancy: 10.9 % / Net I/σ(I): 8.9

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Processing

SoftwareName: PHENIX / Version: (phenix.refine: dev_1769) / Classification: refinement
RefinementResolution: 3.541→49.4 Å / SU ML: 0.46 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 25.86 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.262 4187 5.05 %
Rwork0.2282 --
obs0.2299 82942 92.2 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.541→49.4 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms42143 0 250 0 42393
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00643243
X-RAY DIFFRACTIONf_angle_d1.14958485
X-RAY DIFFRACTIONf_dihedral_angle_d15.21316067
X-RAY DIFFRACTIONf_chiral_restr0.0456632
X-RAY DIFFRACTIONf_plane_restr0.0057352
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.5411-3.58130.3172440.2683689X-RAY DIFFRACTION25
3.5813-3.62340.3457760.26251283X-RAY DIFFRACTION45
3.6234-3.66760.2808850.27641628X-RAY DIFFRACTION59
3.6676-3.7140.27541030.27131982X-RAY DIFFRACTION69
3.714-3.76280.30821380.27422284X-RAY DIFFRACTION81
3.7628-3.81440.31551270.26062535X-RAY DIFFRACTION90
3.8144-3.86880.31921610.27012788X-RAY DIFFRACTION99
3.8688-3.92660.31921540.25822855X-RAY DIFFRACTION100
3.9266-3.98790.24761350.26272829X-RAY DIFFRACTION100
3.9879-4.05330.30051530.25182854X-RAY DIFFRACTION100
4.0533-4.12310.3121470.24382819X-RAY DIFFRACTION100
4.1231-4.1980.27741520.24472824X-RAY DIFFRACTION100
4.198-4.27870.28541490.22832844X-RAY DIFFRACTION100
4.2787-4.3660.26041520.21592818X-RAY DIFFRACTION100
4.366-4.46090.2241570.22132845X-RAY DIFFRACTION100
4.4609-4.56460.23711380.21852888X-RAY DIFFRACTION100
4.5646-4.67870.24381660.22512805X-RAY DIFFRACTION100
4.6787-4.80510.2441600.222837X-RAY DIFFRACTION100
4.8051-4.94630.2651460.22192852X-RAY DIFFRACTION100
4.9463-5.10580.27831460.21962833X-RAY DIFFRACTION100
5.1058-5.28810.26471560.22672825X-RAY DIFFRACTION100
5.2881-5.49960.28151510.2172862X-RAY DIFFRACTION100
5.4996-5.74950.2521620.23032869X-RAY DIFFRACTION100
5.7495-6.05220.27691400.24482833X-RAY DIFFRACTION100
6.0522-6.43060.28121410.24632872X-RAY DIFFRACTION100
6.4306-6.92590.23581480.21992863X-RAY DIFFRACTION100
6.9259-7.62060.2481530.20792860X-RAY DIFFRACTION100
7.6206-8.71810.19771750.17292842X-RAY DIFFRACTION100
8.7181-10.9640.1691270.15722901X-RAY DIFFRACTION100
10.964-49.40510.25651450.23642936X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.2985-0.13010.09410.47530.11240.49090.089-0.04970.01730.1505-0.0077-0.00690.1329-0.0088-0.06580.33580.00580.12830.14260.03160.3363-10.0977-56.016633.154
22.79250.687-1.11451.039-0.39221.34460.0418-0.3843-0.0060.1831-0.0201-0.07210.14720.3193-0.03350.34490.0857-0.04010.3257-0.09020.261840.2601-43.71548.0736
30.2152-0.05860.5490.1533-0.42623.10250.0137-0.0259-0.0407-0.1130.014-0.00340.37490.68090.03270.37360.09360.08680.41-0.02120.543951.0587-39.8656-20.9007
41.19760.0599-0.26370.4951-0.22130.45960.03430.1613-0.03620.00440.04480.12860.0262-0.0804-0.07850.3008-0.01110.00850.1941-0.03010.30228.0678-31.0834-3.4015
51.36370.11740.02420.73850.18070.33770.09610.09720.0056-0.1154-0.1187-0.01250.03130.01780.03390.51840.0702-0.08920.15840.04110.2302-13.344819.219653.6367
62.0378-0.27111.36090.8284-0.5772.2195-0.05830.3780.0035-0.0575-0.0303-0.168-0.10880.46420.04030.39190.01410.07770.3792-0.00290.294538.66276.478149.6171
70.86650.2385-1.06420.4073-1.07722.18410.1755-0.27640.04550.0138-0.03940.043-0.56890.8909-0.11120.4065-0.1709-0.09450.4045-0.05770.447334.79694.0149120.2734
81.10.04910.49120.6248-0.08570.73980.1093-0.11860.09410.0592-0.03880.0108-0.0504-0.0996-0.05940.4460.036-0.01540.1657-0.02510.2873-2.3117-5.534794.4179
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1365 through 2066 )
2X-RAY DIFFRACTION2chain 'A' and (resid 2067 through 2824 )
3X-RAY DIFFRACTION3chain 'A' and (resid 2825 through 3387 )
4X-RAY DIFFRACTION4chain 'A' and (resid 3388 through 4092 )
5X-RAY DIFFRACTION5chain 'B' and (resid 1365 through 2066 )
6X-RAY DIFFRACTION6chain 'B' and (resid 2067 through 2818 )
7X-RAY DIFFRACTION7chain 'B' and (resid 2819 through 3355 )
8X-RAY DIFFRACTION8chain 'B' and (resid 3356 through 4092 )

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