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Yorodumi- PDB-4uus: CRYSTAL STRUCTURE OF A UBX-EXD-DNA COMPLEX INCLUDING THE UBDA MOTIF -
+Open data
-Basic information
Entry | Database: PDB / ID: 4uus | ||||||
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Title | CRYSTAL STRUCTURE OF A UBX-EXD-DNA COMPLEX INCLUDING THE UBDA MOTIF | ||||||
Components |
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Keywords | TRANSCRIPTION / HOMEODOMAIN / HOX PROTEIN / PBC PROTEIN / DNA PROTEIN COMPLEX / TRANSCRIPTION FACTOR | ||||||
Function / homology | Function and homology information dorsal vessel aortic cell fate commitment / positive regulation of muscle organ development / anterior Malpighian tubule development / specification of animal organ identity / regulation of imaginal disc growth / salivary gland boundary specification / haltere development / oenocyte development / mesodermal cell fate specification / specification of segmental identity, thorax ...dorsal vessel aortic cell fate commitment / positive regulation of muscle organ development / anterior Malpighian tubule development / specification of animal organ identity / regulation of imaginal disc growth / salivary gland boundary specification / haltere development / oenocyte development / mesodermal cell fate specification / specification of segmental identity, thorax / open tracheal system development / imaginal disc-derived leg morphogenesis / somatic muscle development / muscle cell fate specification / polytene chromosome band / endoderm formation / eye development / regulation of cell fate specification / peripheral nervous system development / cell fate determination / anterior/posterior pattern specification / midgut development / transcription factor binding / neuron development / embryonic organ development / cis-regulatory region sequence-specific DNA binding / transcription repressor complex / transcription coregulator binding / protein-DNA complex / transcription coregulator activity / animal organ morphogenesis / brain development / RNA polymerase II transcription regulator complex / heart development / DNA-binding transcription factor binding / transcription regulator complex / sequence-specific DNA binding / transcription coactivator activity / DNA-binding transcription factor activity, RNA polymerase II-specific / protein heterodimerization activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / protein domain specific binding / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | DROSOPHILA MELANOGASTER (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.55 Å | ||||||
Authors | Foos, N. / Mate, M.J. / Ortiz-Lombardia, M. | ||||||
Citation | Journal: Structure / Year: 2015 Title: A Flexible Extension of the Drosophila Ultrabithorax Homeodomain Defines a Novel Hox/Pbc Interaction Mode. Authors: Foos, N. / Maurel-Zaffran, C. / Mate, M.J. / Vincentelli, R. / Hainaut, M. / Berenger, H. / Pradel, J. / Saurin, A.J. / Ortiz-Lombardia, M. / Graba, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4uus.cif.gz | 194 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4uus.ent.gz | 153 KB | Display | PDB format |
PDBx/mmJSON format | 4uus.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4uus_validation.pdf.gz | 464.4 KB | Display | wwPDB validaton report |
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Full document | 4uus_full_validation.pdf.gz | 469.7 KB | Display | |
Data in XML | 4uus_validation.xml.gz | 15.4 KB | Display | |
Data in CIF | 4uus_validation.cif.gz | 21.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uu/4uus ftp://data.pdbj.org/pub/pdb/validation_reports/uu/4uus | HTTPS FTP |
-Related structure data
Related structure data | 4cycC 4uutC 1b8iS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 9591.063 Da / Num. of mol.: 2 / Fragment: HOMEODOMAIN AND UBDA, RESIDUES 292-367 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) DROSOPHILA MELANOGASTER (fruit fly) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA PLYSS / References: UniProt: P83949 #2: Protein | Mass: 8857.946 Da / Num. of mol.: 2 / Fragment: HOMEODOMAIN RESIDUES 238-312 Source method: isolated from a genetically manipulated source Source: (gene. exp.) DROSOPHILA MELANOGASTER (fruit fly) / Plasmid: EXD2 PETG20A / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA PLYSS / References: UniProt: P40427 #3: DNA chain | Mass: 4537.975 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: UBX-EXD CONSENSUS BINDING SITE / Source: (synth.) DROSOPHILA MELANOGASTER (fruit fly) #4: DNA chain | Mass: 4640.020 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: UBX-EXD CONSENSUS BINDING SITE / Source: (synth.) DROSOPHILA MELANOGASTER (fruit fly) #5: Water | ChemComp-HOH / | Sequence details | FIRST G FROM FUSION TAG AFTER CLEAVAGE | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.56 Å3/Da / Density % sol: 57.16 % / Description: NONE |
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Crystal grow | pH: 4.5 Details: 0.2M AMONIUM SULFATE, 0.1 M TRI-SODIUM-CITRATE PH 4.5, 4% P4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8726 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Oct 23, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 2.55→38.55 Å / Num. obs: 18169 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / Redundancy: 5.3 % / Biso Wilson estimate: 57.22 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 13.6 |
Reflection shell | Resolution: 2.55→2.69 Å / Redundancy: 5.3 % / Rmerge(I) obs: 0.58 / Mean I/σ(I) obs: 3.4 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1B8I Resolution: 2.55→38.55 Å / Cor.coef. Fo:Fc: 0.9291 / Cor.coef. Fo:Fc free: 0.8915 / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 39.09 Å2
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Refine analyze | Luzzati coordinate error obs: 0.36 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.55→38.55 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.55→2.71 Å / Total num. of bins used: 9
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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