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基本情報
登録情報 | データベース: PDB / ID: 4uqk | ||||||
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タイトル | Electron density map of GluA2em in complex with quisqualate and LY451646 | ||||||
![]() | GLUTAMATE RECEPTOR 2 | ||||||
![]() | TRANSPORT PROTEIN / GLUA2EM RESTORED ACTIVE STATE | ||||||
機能・相同性 | ![]() spine synapse / dendritic spine neck / dendritic spine head / Activation of AMPA receptors / perisynaptic space / AMPA glutamate receptor activity / Trafficking of GluR2-containing AMPA receptors / response to lithium ion / immunoglobulin binding / AMPA glutamate receptor complex ...spine synapse / dendritic spine neck / dendritic spine head / Activation of AMPA receptors / perisynaptic space / AMPA glutamate receptor activity / Trafficking of GluR2-containing AMPA receptors / response to lithium ion / immunoglobulin binding / AMPA glutamate receptor complex / kainate selective glutamate receptor activity / ionotropic glutamate receptor complex / extracellularly glutamate-gated ion channel activity / cellular response to glycine / asymmetric synapse / regulation of receptor recycling / Unblocking of NMDA receptors, glutamate binding and activation / glutamate receptor binding / positive regulation of synaptic transmission / glutamate-gated receptor activity / presynaptic active zone membrane / response to fungicide / regulation of synaptic transmission, glutamatergic / somatodendritic compartment / cellular response to brain-derived neurotrophic factor stimulus / dendrite membrane / ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / cytoskeletal protein binding / ionotropic glutamate receptor signaling pathway / dendrite cytoplasm / SNARE binding / dendritic shaft / synaptic membrane / synaptic transmission, glutamatergic / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / PDZ domain binding / protein tetramerization / postsynaptic density membrane / ionotropic glutamate receptor binding / Schaffer collateral - CA1 synapse / modulation of chemical synaptic transmission / establishment of protein localization / terminal bouton / receptor internalization / cerebral cortex development / synaptic vesicle membrane / synaptic vesicle / presynapse / presynaptic membrane / signaling receptor activity / amyloid-beta binding / growth cone / scaffold protein binding / chemical synaptic transmission / postsynaptic membrane / perikaryon / dendritic spine / postsynaptic density / neuron projection / axon / neuronal cell body / glutamatergic synapse / synapse / dendrite / protein-containing complex binding / endoplasmic reticulum membrane / protein kinase binding / cell surface / endoplasmic reticulum / protein-containing complex / identical protein binding / membrane / plasma membrane 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 16.4 Å | ||||||
![]() | Meyerson, J.R. / Kumar, J. / Chittori, S. / Rao, P. / Pierson, J. / Bartesaghi, A. / Mayer, M.L. / Subramaniam, S. | ||||||
![]() | ![]() タイトル: Structural mechanism of glutamate receptor activation and desensitization. 著者: Joel R Meyerson / Janesh Kumar / Sagar Chittori / Prashant Rao / Jason Pierson / Alberto Bartesaghi / Mark L Mayer / Sriram Subramaniam / ![]() 要旨: Ionotropic glutamate receptors are ligand-gated ion channels that mediate excitatory synaptic transmission in the vertebrate brain. To gain a better understanding of how structural changes gate ion ...Ionotropic glutamate receptors are ligand-gated ion channels that mediate excitatory synaptic transmission in the vertebrate brain. To gain a better understanding of how structural changes gate ion flux across the membrane, we trapped rat AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid) and kainate receptor subtypes in their major functional states and analysed the resulting structures using cryo-electron microscopy. We show that transition to the active state involves a 'corkscrew' motion of the receptor assembly, driven by closure of the ligand-binding domain. Desensitization is accompanied by disruption of the amino-terminal domain tetramer in AMPA, but not kainate, receptors with a two-fold to four-fold symmetry transition in the ligand-binding domains in both subtypes. The 7.6 Å structure of a desensitized kainate receptor shows how these changes accommodate channel closing. These findings integrate previous physiological, biochemical and structural analyses of glutamate receptors and provide a molecular explanation for key steps in receptor gating. | ||||||
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構造の表示
ムービー |
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構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 416.1 KB | 表示 | ![]() |
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PDB形式 | ![]() | 332.3 KB | 表示 | ![]() |
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その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 793.7 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 811.5 KB | 表示 | |
XML形式データ | ![]() | 62.9 KB | 表示 | |
CIF形式データ | ![]() | 100 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 2689MC ![]() 2680C ![]() 2684C ![]() 2685C ![]() 2686C ![]() 2687C ![]() 2688C ![]() 4uq6C ![]() 4uqjC ![]() 4uqqC C: 同じ文献を引用 ( M: このデータのモデリングに利用したマップデータ |
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類似構造データ |
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リンク
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集合体
登録構造単位 | ![]()
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要素
#1: タンパク質 | 分子量: 93105.156 Da / 分子数: 4 / 断片: RESIDUES 22-847 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() 発現宿主: ![]() ![]() 参照: UniProt: P19491 #2: 化合物 | ChemComp-QUS / ( 配列の詳細 | SEQUENCE IS FROM PDB 3KG2 TRUNCATED TO THAT USED TO FIT EM MAPS | |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: GLU A2 ATD AND GLUA2 LBD / タイプ: COMPLEX |
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緩衝液 | pH: 8 |
試料 | 濃度: 1.8 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
試料支持 | 詳細: HOLEY CARBON |
急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE / 詳細: ETHANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS / 日付: 2013年8月1日 |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD / 倍率(公称値): 47000 X / 倍率(補正後): 47000 X / 最大 デフォーカス(公称値): 3500 nm / 最小 デフォーカス(公称値): 2000 nm |
撮影 | 電子線照射量: 25 e/Å2 フィルム・検出器のモデル: FEI FALCON II (4k x 4k) |
画像スキャン | デジタル画像の数: 1303 |
放射波長 | 相対比: 1 |
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解析
EMソフトウェア |
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CTF補正 | 詳細: EACH PARTICLE | ||||||||||||
対称性 | 点対称性: C2 (2回回転対称) | ||||||||||||
3次元再構成 | 解像度: 16.4 Å / 粒子像の数: 4795 詳細: FOUR ASSEMBLIES CONSISTING OF TWO ATD DIMERS FROM PDB 3KG2 AND TWO LBD DIMERS FROM PDB 1MM7 WERE FIT AS INDEPENDENT RIGID BODIES TO EM MAPS GEOMETRY AND STEREOCHEMISTRY OUTLIERS ARE THOSE ...詳細: FOUR ASSEMBLIES CONSISTING OF TWO ATD DIMERS FROM PDB 3KG2 AND TWO LBD DIMERS FROM PDB 1MM7 WERE FIT AS INDEPENDENT RIGID BODIES TO EM MAPS GEOMETRY AND STEREOCHEMISTRY OUTLIERS ARE THOSE PRESENT IN THE PDB ENTRIES USED FOR RIGID BODY FITTING SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2689. (DEPOSITION ID: 12609). 対称性のタイプ: POINT | ||||||||||||
原子モデル構築 | プロトコル: RIGID BODY FIT / 空間: REAL / 詳細: METHOD--RIGID BODY REFINEMENT PROTOCOL--RIGID BODY | ||||||||||||
原子モデル構築 | PDB-ID: 1MM7 | ||||||||||||
精密化 | 最高解像度: 16.4 Å | ||||||||||||
精密化ステップ | サイクル: LAST / 最高解像度: 16.4 Å
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