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- PDB-4ue9: Complex of D. melanogaster eIF4E with the 4E-binding protein 4E-T -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ue9 | ||||||
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Title | Complex of D. melanogaster eIF4E with the 4E-binding protein 4E-T | ||||||
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![]() | TRANSLATION / GENE REGULATION / CAP BINDING PROTEIN / 4E BINDING PROTEIN / TRANSLATIONAL REPRESSION | ||||||
Function / homology | ![]() negative regulation of eukaryotic translation initiation factor 4F complex assembly / TOR signaling pathway / Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / ISG15 antiviral mechanism / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / Transport of Mature mRNA Derived from an Intronless Transcript / mTORC1-mediated signalling / Ribosomal scanning and start codon recognition / L13a-mediated translational silencing of Ceruloplasmin expression ...negative regulation of eukaryotic translation initiation factor 4F complex assembly / TOR signaling pathway / Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / ISG15 antiviral mechanism / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / Transport of Mature mRNA Derived from an Intronless Transcript / mTORC1-mediated signalling / Ribosomal scanning and start codon recognition / L13a-mediated translational silencing of Ceruloplasmin expression / Translation initiation complex formation / muscle cell postsynaptic specialization / trans-synaptic signaling / neuronal ribonucleoprotein granule / RNA metabolic process / eukaryotic initiation factor 4G binding / eukaryotic initiation factor 4E binding / regulation of translation at postsynapse, modulating synaptic transmission / RNA cap binding / eukaryotic translation initiation factor 4F complex / RNA 7-methylguanosine cap binding / translational initiation / translation initiation factor activity / P-body / neuromuscular junction / postsynapse / nuclear body / negative regulation of translation / translation / mRNA binding / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Peter, D. / Weichenrieder, O. | ||||||
![]() | ![]() Title: Molecular Architecture of 4E-BP Translational Inhibitors Bound to Eif4E. Authors: Peter, D. / Igreja, C. / Weber, R. / Wohlbold, L. / Weiler, C. / Ebertsch, L. / Weichenrieder, O. / Izaurralde, E. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 91.2 KB | Display | ![]() |
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PDB format | ![]() | 71 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 428 KB | Display | ![]() |
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Full document | ![]() | 429.1 KB | Display | |
Data in XML | ![]() | 9.6 KB | Display | |
Data in CIF | ![]() | 12.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4ue8SC ![]() 4ueaC ![]() 4uebC ![]() 4uecC ![]() 4uedC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21249.037 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 80-259 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 4772.581 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 9-44 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#3: Water | ChemComp-HOH / |
Sequence details | THE FIRST FOUR RESIDUES OF CHAIN A REMAIN FROM THE EXPRESSION TAG. COMPARED TO UNP P48598, SEQUENCE ...THE FIRST FOUR RESIDUES OF CHAIN A REMAIN FROM THE EXPRESSION |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41 % / Description: NONE |
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Crystal grow | pH: 8.5 / Details: 0.1M TRIS-HCL PH 8.5, 0.05M LISO4, 28% PEG400 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 6, 2014 / Details: DYNAMICALLY BENDABLE MIRRORS |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→36.9 Å / Num. obs: 12812 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 34.7 % / Biso Wilson estimate: 43.25 Å2 / Rsym value: 0.09 / Net I/σ(I): 25.6 |
Reflection shell | Resolution: 2.15→2.21 Å / Redundancy: 31.4 % / Mean I/σ(I) obs: 4.5 / Rsym value: 0.71 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4UE8 CHAIN A Resolution: 2.15→36.922 Å / SU ML: 0.25 / σ(F): 1.4 / Phase error: 27.88 / Stereochemistry target values: ML Details: HYDROGENS WERE REFINED IN THE RIDING POSITIONS. THE SIDECHAINS OF THE FOLLOWING RESIDUES WERE TRUNCATED AT C-BETA ATOMS. CHAIN A, RESIDUES 88, 89. CHAIN B, RESIDUES 25, 29, 33. THE FOLLOWING ...Details: HYDROGENS WERE REFINED IN THE RIDING POSITIONS. THE SIDECHAINS OF THE FOLLOWING RESIDUES WERE TRUNCATED AT C-BETA ATOMS. CHAIN A, RESIDUES 88, 89. CHAIN B, RESIDUES 25, 29, 33. THE FOLLOWING RESIDUES ARE DISORDERED. CHAIN A, RESIDUES 86 TO 87, 237 TO 240. CHAIN B, RESIDUES 26 TO 28.
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 50.03 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.15→36.922 Å
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Refine LS restraints |
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LS refinement shell |
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