+Open data
-Basic information
Entry | Database: PDB / ID: 4u8h | ||||||||||||
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Title | Crystal Structure of Mammalian Period-Cryptochrome Complex | ||||||||||||
Components |
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Keywords | CIRCADIAN CLOCK PROTEIN/TRANSCRIPTION / transcriptional repression / zinc-binding / CIRCADIAN CLOCK PROTEIN-TRANSCRIPTION complex | ||||||||||||
Function / homology | Function and homology information regulation of glutamate uptake involved in transmission of nerve impulse / regulation of sodium-dependent phosphate transport / circadian regulation of translation / negative regulation of termination of DNA-templated transcription / negative regulation of glucocorticoid secretion / negative regulation of fat cell proliferation / negative regulation of glucocorticoid receptor signaling pathway / negative regulation of transcription regulatory region DNA binding / negative regulation of circadian rhythm / lactate biosynthetic process ...regulation of glutamate uptake involved in transmission of nerve impulse / regulation of sodium-dependent phosphate transport / circadian regulation of translation / negative regulation of termination of DNA-templated transcription / negative regulation of glucocorticoid secretion / negative regulation of fat cell proliferation / negative regulation of glucocorticoid receptor signaling pathway / negative regulation of transcription regulatory region DNA binding / negative regulation of circadian rhythm / lactate biosynthetic process / histone methyltransferase binding / lipid storage / pre-mRNA binding / glycogen biosynthetic process / RNA polymerase binding / entrainment of circadian clock by photoperiod / photoreceptor activity / regulation of insulin secretion / response to light stimulus / regulation of vasoconstriction / white fat cell differentiation / regulation of neurogenesis / phosphatase binding / negative regulation of protein ubiquitination / FAD binding / transcription corepressor binding / fatty acid metabolic process / response to activity / response to ischemia / gluconeogenesis / nuclear receptor binding / circadian regulation of gene expression / response to insulin / regulation of circadian rhythm / kinase binding / protein import into nucleus / circadian rhythm / histone deacetylase binding / single-stranded DNA binding / glucose homeostasis / positive regulation of cold-induced thermogenesis / DNA-binding transcription factor binding / damaged DNA binding / transcription coactivator activity / transcription cis-regulatory region binding / regulation of cell cycle / nuclear speck / chromatin remodeling / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of DNA-templated transcription / protein kinase binding / perinuclear region of cytoplasm / negative regulation of transcription by RNA polymerase II / mitochondrion / DNA binding / extracellular region / nucleoplasm / identical protein binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||||||||
Biological species | Mus musculus (house mouse) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.798 Å | ||||||||||||
Authors | Nangle, S.N. / Rosensweig, C. / Koike, N. / Tei, H. / Takahashi, J.S. / Green, C.B. / Zheng, N. | ||||||||||||
Funding support | United States, 3items
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Citation | Journal: Elife / Year: 2014 Title: Molecular assembly of the period-cryptochrome circadian transcriptional repressor complex. Authors: Nangle, S.N. / Rosensweig, C. / Koike, N. / Tei, H. / Takahashi, J.S. / Green, C.B. / Zheng, N. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4u8h.cif.gz | 480.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4u8h.ent.gz | 395.3 KB | Display | PDB format |
PDBx/mmJSON format | 4u8h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u8/4u8h ftp://data.pdbj.org/pub/pdb/validation_reports/u8/4u8h | HTTPS FTP |
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-Related structure data
Related structure data | 4mlpS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 58368.676 Da / Num. of mol.: 2 Fragment: Photolyase/cryptochrome alpha/beta domain, residues 1-510 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Cry2, Kiaa0658 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q9R194 #2: Protein | Mass: 13935.588 Da / Num. of mol.: 2 / Fragment: CRY binding domain, residues 1095-1215 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Per2 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: O54943 #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.31 % / Description: Diamond |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.5 / Details: 0.1 M HEPES, 0.2 M NaCl, 15% PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 28, 2013 |
Radiation | Monochromator: Double crystal, Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.798→42.738 Å / Num. obs: 37522 / % possible obs: 99.59 % / Redundancy: 4.2 % / Net I/σ(I): 18.8 |
-Processing
Software | Name: PHENIX / Version: (phenix.refine: 1.8.2_1309) / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4MLP Resolution: 2.798→42.738 Å / SU ML: 0.48 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 32.18 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.798→42.738 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 65.4943 Å / Origin y: 24.4242 Å / Origin z: -8.6096 Å
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Refinement TLS group | Selection details: all |