- PDB-4qmi: The XMAP215 family drives microtubule polymerization using a stru... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 4qmi
Title
The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array
Components
Cytoskeleton-associated protein 5
Keywords
PROTEIN BINDING / TOG domain
Function / homology
Function and homology information
microtubule plus end polymerase / establishment or maintenance of microtubule cytoskeleton polarity / RNA transport / positive regulation of microtubule nucleation / microtubule plus-end binding / centrosome cycle / microtubule depolymerization / spindle organization / microtubule polymerization / centrosome duplication ...microtubule plus end polymerase / establishment or maintenance of microtubule cytoskeleton polarity / RNA transport / positive regulation of microtubule nucleation / microtubule plus-end binding / centrosome cycle / microtubule depolymerization / spindle organization / microtubule polymerization / centrosome duplication / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / ribonucleoprotein complex binding / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Recruitment of NuMA to mitotic centrosomes / Resolution of Sister Chromatid Cohesion / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / mitotic spindle organization / RHO GTPases Activate Formins / kinetochore / spindle pole / Separation of Sister Chromatids / Regulation of PLK1 Activity at G2/M Transition / microtubule binding / cadherin binding / cell division / centrosome / nucleolus / protein-containing complex / membrane / plasma membrane / cytoplasm / cytosol Similarity search - Function
XMAP215 family / : / XMAP215/Dis1/CLASP, TOG domain / CLASP N-terminal domain / CLASP N terminal / TOG domain / TOG / HEAT repeat profile. / HEAT, type 2 / Leucine-rich Repeat Variant ...XMAP215 family / : / XMAP215/Dis1/CLASP, TOG domain / CLASP N-terminal domain / CLASP N terminal / TOG domain / TOG / HEAT repeat profile. / HEAT, type 2 / Leucine-rich Repeat Variant / Leucine-rich Repeat Variant / Armadillo-like helical / Alpha Horseshoe / Armadillo-type fold / Mainly Alpha Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 360 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.1 Å3/Da / Density % sol: 41.33 %
Crystal grow
Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 10 mg/mL protein was added to an equal volume of a mother liquor containing 28% PEG 4000, 100 mM Tris pH 7.5, and 125 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
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