PeptidoglycanendopeptidaseRipA / Macrophage invasion and intracellular persistence protein A / Resuscitation-promoting factor ...Macrophage invasion and intracellular persistence protein A / Resuscitation-promoting factor interaction partner A / Rpf-interacting protein A
Mass: 49802.828 Da / Num. of mol.: 1 / Fragment: RipA / Mutation: E444A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Strain: ATCC 25618 / H37Rv / Gene: ripA, Rv1477 / Production host: Escherichia coli (E. coli) References: UniProt: O53168, Hydrolases; Acting on peptide bonds (peptidases)
Mass: 18.015 Da / Num. of mol.: 259 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 8 mg mL-1 protein concentration and 2.0 M sodium formate in 0.1 M sodium acetate trihydrate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.54 Å