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- PDB-4pjj: MYOSIN VI (MD-INSERT2-CAM, DELTA-INSERT1) post-rigor state - long... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4pjj | ||||||||||||
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Title | MYOSIN VI (MD-INSERT2-CAM, DELTA-INSERT1) post-rigor state - long soaking with PO4 | ||||||||||||
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![]() | MOTOR PROTEIN / MYOSIN VI / POST-RIGOR STATE / MG.ADP.BEFX / CALMODULIN / MOLECULAR MOTOR | ||||||||||||
Function / homology | ![]() negative regulation of phospholipase C-activating phototransduction signaling pathway / myosin VI complex / myosin VI head/neck binding / myosin VII complex / photoreceptor cell axon guidance / negative regulation of opsin-mediated signaling pathway / rhabdomere development / detection of chemical stimulus involved in sensory perception of smell / rhabdomere / myosin V complex ...negative regulation of phospholipase C-activating phototransduction signaling pathway / myosin VI complex / myosin VI head/neck binding / myosin VII complex / photoreceptor cell axon guidance / negative regulation of opsin-mediated signaling pathway / rhabdomere development / detection of chemical stimulus involved in sensory perception of smell / rhabdomere / myosin V complex / kinetochore organization / autophagic cell death / G protein-coupled opsin signaling pathway / actin filament-based movement / myosin V binding / channel regulator activity / myosin complex / myosin heavy chain binding / muscle cell cellular homeostasis / mitotic spindle pole / microvillus / centriole replication / cytoskeletal motor activity / cellular response to ethanol / enzyme regulator activity / clathrin-coated pit / centriole / sensory perception of sound / microtubule cytoskeleton organization / spindle / ruffle membrane / actin filament binding / mitotic spindle / sensory perception of smell / midbody / cell cortex / calmodulin binding / calcium ion binding / centrosome / nucleoplasm / ATP binding / metal ion binding / cytosol / cytoplasm Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Isabet, T. / Benisty, H. / Llinas, P. / Sweeney, H.L. / Houdusse, A. | ||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: How actin initiates the motor activity of Myosin. Authors: Llinas, P. / Isabet, T. / Song, L. / Ropars, V. / Zong, B. / Benisty, H. / Sirigu, S. / Morris, C. / Kikuti, C. / Safer, D. / Sweeney, H.L. / Houdusse, A. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 383.1 KB | Display | ![]() |
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PDB format | ![]() | 305.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 792.4 KB | Display | ![]() |
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Full document | ![]() | 801.5 KB | Display | |
Data in XML | ![]() | 35.4 KB | Display | |
Data in CIF | ![]() | 51.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4pfoC ![]() 4pfpC ![]() 4pjkC ![]() 4pjlC ![]() 4pjmC ![]() 4pjnC ![]() 4pk4C ![]() 2vasS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 89873.477 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 16841.520 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Non-polymers , 6 types, 362 molecules 










#3: Chemical | ChemComp-ADP / | ||||||
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#4: Chemical | ChemComp-MG / | ||||||
#5: Chemical | #6: Chemical | #7: Chemical | #8: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.37 Å3/Da / Density % sol: 63.49 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 9% PEG8k, 50mM Hepes pH 7,5, 1mM TCEP, 3% EG, 3% MPD PH range: 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 5, 2011 |
Radiation | Monochromator: Si III / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9801 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→45 Å / Num. obs: 57130 / % possible obs: 99.8 % / Redundancy: 9.57 % / Biso Wilson estimate: 57.71 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 14.23 |
Reflection shell | Resolution: 2.4→2.46 Å / Redundancy: 9.69 % / Rmerge(I) obs: 1.076 / Mean I/σ(I) obs: 2.31 / % possible all: 98.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2VAS Resolution: 2.4→43.74 Å / Cor.coef. Fo:Fc: 0.9524 / Cor.coef. Fo:Fc free: 0.9387 / SU R Cruickshank DPI: 0.217 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.222 / SU Rfree Blow DPI: 0.179 / SU Rfree Cruickshank DPI: 0.179
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Displacement parameters | Biso mean: 59.36 Å2
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Refine analyze | Luzzati coordinate error obs: 0.293 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 2.4→43.74 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.46 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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