Crystal structure of mannohexaose bound oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1223) from THERMOTOGA MARITIMA at 1.5 A resolution
要素
ABC transporter substrate-binding protein
キーワード
TRANSPORT PROTEIN / oligopeptide ABC transporter / periplasmic oligopeptide-binding protein
機能・相同性
機能・相同性情報
peptide transport / peptide transmembrane transporter activity / ATP-binding cassette (ABC) transporter complex / outer membrane-bounded periplasmic space / metal ion binding 類似検索 - 分子機能
Dipeptide-binding Protein; domain 1 / Dipeptide-binding Protein; Domain 1 / Dipeptide-binding Protein; domain 3 / Dipeptide-binding Protein; Domain 3 / Peptide/nickel binding protein, MppA-type / Solute-binding protein family 5 domain / Solute-binding protein family 5 / Bacterial extracellular solute-binding proteins, family 5 Middle / Periplasmic binding protein-like II / D-Maltodextrin-Binding Protein; domain 2 ...Dipeptide-binding Protein; domain 1 / Dipeptide-binding Protein; Domain 1 / Dipeptide-binding Protein; domain 3 / Dipeptide-binding Protein; Domain 3 / Peptide/nickel binding protein, MppA-type / Solute-binding protein family 5 domain / Solute-binding protein family 5 / Bacterial extracellular solute-binding proteins, family 5 Middle / Periplasmic binding protein-like II / D-Maltodextrin-Binding Protein; domain 2 / Roll / Alpha-Beta Complex / 3-Layer(aba) Sandwich / Alpha Beta 類似検索 - ドメイン・相同性
NITRATE ION / Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein 類似検索 - 構成要素
ABCtransportersubstrate-bindingprotein / Mannoside ABC transport system / sugar-binding protein / Oligopeptide ABC transporter / periplasmic ...Mannoside ABC transport system / sugar-binding protein / Oligopeptide ABC transporter / periplasmic oligopeptide-binding protein
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.5418 Å / 相対比: 1
反射
解像度: 1.5→99.53 Å / Num. obs: 163023 / % possible obs: 99.9 % / 冗長度: 3.5 % / Net I/σ(I): 15.7
反射 シェル
解像度: 1.5→1.54 Å / 冗長度: 3.3 % / Rmerge(I) obs: 0.604 / Mean I/σ(I) obs: 2.5 / % possible all: 99.4
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解析
ソフトウェア
名称: REFMAC / バージョン: 5.8.0049 / 分類: 精密化
精密化
構造決定の手法: 分子置換 / 解像度: 1.5→99.53 Å / Cor.coef. Fo:Fc: 0.973 / Cor.coef. Fo:Fc free: 0.965 / SU B: 1.508 / SU ML: 0.055 / 交差検証法: THROUGHOUT / ESU R: 0.076 / ESU R Free: 0.075 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.18723
8181
5 %
RANDOM
Rwork
0.16263
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-
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obs
0.16388
154816
99.78 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK