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- PDB-4ou9: Crystal structure of apocarotenoid oxygenase in the presence of T... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ou9 | ||||||
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Title | Crystal structure of apocarotenoid oxygenase in the presence of Triton X-100 | ||||||
![]() | Apocarotenoid-15,15'-oxygenase | ||||||
![]() | OXIDOREDUCTASE / monotopic membrane protein / non-heme iron / metalloenzyme / 4-His iron center / beta propeller / carotenoid oxygenase | ||||||
Function / homology | ![]() all-trans-8'-apo-beta-carotenal 15,15'-oxygenase / all-trans-8'-apo-beta-carotenal 15,15'-oxygenase activity / carotenoid dioxygenase activity / 9-cis-epoxycarotenoid dioxygenase activity / carotene catabolic process / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sui, X. / Palczewski, K. / Kiser, P.D. | ||||||
![]() | ![]() Title: Analysis of Carotenoid Isomerase Activity in a Prototypical Carotenoid Cleavage Enzyme, Apocarotenoid Oxygenase (ACO). Authors: Sui, X. / Kiser, P.D. / Che, T. / Carey, P.R. / Golczak, M. / Shi, W. / von Lintig, J. / Palczewski, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 787.2 KB | Display | ![]() |
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PDB format | ![]() | 660 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 452.3 KB | Display | ![]() |
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Full document | ![]() | 460.7 KB | Display | |
Data in XML | ![]() | 72.7 KB | Display | |
Data in CIF | ![]() | 104.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 54344.191 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P74334, all-trans-8'-apo-beta-carotenal 15,15'-oxygenase #2: Chemical | ChemComp-FE2 / #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.46 Å3/Da / Density % sol: 64.45 % |
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Crystal grow | Temperature: 281 K / Method: vapor diffusion, hanging drop / pH: 6 Details: PEG 3350, NH4Cl, Triton X-100, BTP, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Nov 10, 2013 |
Radiation | Monochromator: Silicon(111) crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. all: 203162 / Num. obs: 200469 / % possible obs: 98.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.7 Å / Shrinkage radii: 0.7 Å / VDW probe radii: 1.1 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 46.673 Å2
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Refinement step | Cycle: LAST / Resolution: 2→48.61 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.052 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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