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- PDB-4ogx: Crystal structure of Fab DX-2930 in complex with human plasma kal... -
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Basic information
Entry | Database: PDB / ID: 4ogx | ||||||
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Title | Crystal structure of Fab DX-2930 in complex with human plasma kallikrein at 2.4 Angstrom resolution | ||||||
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![]() | HYDROLASE/ANTIBODY / FAB / ANTIBODY / KALLIKREIN / BLOOD / PLASMA / PLASMA KALLIKREIN- MEDIATED EDEMA / ACUTE HEREDITARY ANGIOEDEMA / HAE / HMWK / serpin C1-inhibitor / C1-INH / hereditary angioedema / HAW / bradykinin / Fletcher factor / Kininogenin / serine protease / edema / HYDROLASE-ANTIBODY complex | ||||||
Function / homology | ![]() plasma kallikrein / Factor XII activation / Defective SERPING1 causes hereditary angioedema / positive regulation of fibrinolysis / immunoglobulin complex / zymogen activation / plasminogen activation / Defective factor XII causes hereditary angioedema / Activation of Matrix Metalloproteinases / immunoglobulin mediated immune response ...plasma kallikrein / Factor XII activation / Defective SERPING1 causes hereditary angioedema / positive regulation of fibrinolysis / immunoglobulin complex / zymogen activation / plasminogen activation / Defective factor XII causes hereditary angioedema / Activation of Matrix Metalloproteinases / immunoglobulin mediated immune response / fibrinolysis / Intrinsic Pathway of Fibrin Clot Formation / antigen binding / blood coagulation / blood microparticle / immune response / serine-type endopeptidase activity / proteolysis / extracellular space / extracellular exosome / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Edwards, T.E. / Clifton, M.C. / Abendroth, J. / Nixon, A. / Ladner, R. | ||||||
![]() | ![]() Title: Inhibition of plasma kallikrein by a highly specific active site blocking antibody. Authors: Kenniston, J.A. / Faucette, R.R. / Martik, D. / Comeau, S.R. / Lindberg, A.P. / Kopacz, K.J. / Conley, G.P. / Chen, J. / Viswanathan, M. / Kastrapeli, N. / Cosic, J. / Mason, S. / DiLeo, M. ...Authors: Kenniston, J.A. / Faucette, R.R. / Martik, D. / Comeau, S.R. / Lindberg, A.P. / Kopacz, K.J. / Conley, G.P. / Chen, J. / Viswanathan, M. / Kastrapeli, N. / Cosic, J. / Mason, S. / DiLeo, M. / Abendroth, J. / Kuzmic, P. / Ladner, R.C. / Edwards, T.E. / TenHoor, C. / Adelman, B.A. / Nixon, A.E. / Sexton, D.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 147.7 KB | Display | ![]() |
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PDB format | ![]() | 113.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4ogyC ![]() 4pubC ![]() 2anyS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27144.842 Da / Num. of mol.: 1 / Fragment: UNP residues 391-631 / Mutation: N396E, N453E, N494E, C503S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Antibody | Mass: 24039.129 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
#3: Antibody | Mass: 23446.020 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
#4: Chemical | ChemComp-SO4 / #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 54 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: FAB DX-2930 AND HUMAN PLASMA KALLIKREIN VCID 7481 AT 150 UM OR 11.7 MG/ML AGAINST WIZ3-4 screen condition H7, 30% MPD, 10% PEG 3350, 0.1 M imidazole pH 6.5, 0.2 M ammonium sulfate, unique ...Details: FAB DX-2930 AND HUMAN PLASMA KALLIKREIN VCID 7481 AT 150 UM OR 11.7 MG/ML AGAINST WIZ3-4 screen condition H7, 30% MPD, 10% PEG 3350, 0.1 M imidazole pH 6.5, 0.2 M ammonium sulfate, unique puck ID QJO1-12, crystal tracking ID 242118H7 , VAPOR DIFFUSION, SITTING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU SATURN 944+ / Detector: CCD / Date: Feb 21, 2013 / Details: VARIMAX |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. all: 32254 / Num. obs: 32218 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / Redundancy: 6.7 % / Biso Wilson estimate: 30.87 Å2 / Rmerge(I) obs: 0.112 / Net I/σ(I): 15.74 |
Reflection shell | Resolution: 2.4→2.45 Å / Redundancy: 4 % / Rmerge(I) obs: 0.477 / Mean I/σ(I) obs: 2.9 / Num. measured obs: 2422 / Num. unique obs: 426 / % possible all: 99.6 |
-Phasing
Phasing | Method: ![]() |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2ANY Resolution: 2.4→41.12 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.901 / SU B: 6.883 / SU ML: 0.162 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.344 / ESU R Free: 0.241 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 78.45 Å2 / Biso mean: 25.07 Å2 / Biso min: 3.9 Å2
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Refinement step | Cycle: LAST / Resolution: 2.4→41.12 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.46 Å / Total num. of bins used: 20
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