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- PDB-4nqq: Crystal structure of mouse P-cadherin extracellular domains EC1-EC2 -
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Open data
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Basic information
Entry | Database: PDB / ID: 4nqq | ||||||
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Title | Crystal structure of mouse P-cadherin extracellular domains EC1-EC2 | ||||||
![]() | Cadherin-3 | ||||||
![]() | CELL ADHESION / Extracellular cadherin (EC) domain / Cell-Cell adhesion | ||||||
Function / homology | ![]() negative regulation of transforming growth factor beta2 production / : / negative regulation of timing of catagen / positive regulation of melanosome transport / hair cycle process / positive regulation of tyrosinase activity / positive regulation of keratinocyte proliferation / Adherens junctions interactions / positive regulation of melanin biosynthetic process / response to xenobiotic stimulus => GO:0009410 ...negative regulation of transforming growth factor beta2 production / : / negative regulation of timing of catagen / positive regulation of melanosome transport / hair cycle process / positive regulation of tyrosinase activity / positive regulation of keratinocyte proliferation / Adherens junctions interactions / positive regulation of melanin biosynthetic process / response to xenobiotic stimulus => GO:0009410 / cell-cell adhesion via plasma-membrane adhesion molecules / catenin complex / retina homeostasis / positive regulation of insulin-like growth factor receptor signaling pathway / keratinization / homophilic cell adhesion via plasma membrane adhesion molecules / adherens junction / wound healing / cell-cell adhesion / positive regulation of canonical Wnt signaling pathway / cell junction / membrane => GO:0016020 / cadherin binding / calcium ion binding / positive regulation of gene expression / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Brasch, J. / Shapiro, L. | ||||||
![]() | ![]() Title: Structural and energetic determinants of adhesive binding specificity in type I cadherins. Authors: Vendome, J. / Felsovalyi, K. / Song, H. / Yang, Z. / Jin, X. / Brasch, J. / Harrison, O.J. / Ahlsen, G. / Bahna, F. / Kaczynska, A. / Katsamba, P.S. / Edmond, D. / Hubbell, W.L. / Shapiro, L. / Honig, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 309.8 KB | Display | ![]() |
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PDB format | ![]() | 256.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 459.5 KB | Display | ![]() |
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Full document | ![]() | 475.6 KB | Display | |
Data in XML | ![]() | 32.4 KB | Display | |
Data in CIF | ![]() | 43.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4numC ![]() 4nupC ![]() 4nuqC ![]() 2qviS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23481.229 Da / Num. of mol.: 4 Fragment: extracellular domains EC1-EC2, UNP residues 100-312 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-CA / #3: Chemical | ChemComp-CU / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.29 Å3/Da / Density % sol: 62.57 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 38% PEG 6000, Bis-Tris pH 6.5, 0.35M Calcium chloride, 0.01M Copper (II) chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Nov 2, 2012 |
Radiation | Monochromator: Bent single Si(111) crystal (horizontal focusing and deflection) Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→40 Å / Num. all: 21058 / Num. obs: 20958 / % possible obs: 99.3 % / Observed criterion σ(F): -3 / Redundancy: 6.3 % |
Reflection shell | Resolution: 3.2→3.31 Å / Redundancy: 6.1 % / Rmerge(I) obs: 0.589 / Mean I/σ(I) obs: 2.6 / % possible all: 97.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 2QVI Resolution: 3.2→20 Å / SU ML: 0.37 / σ(F): 1.34 / Phase error: 27.63 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.2→20 Å
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Refine LS restraints |
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LS refinement shell |
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