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Open data
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Basic information
Entry | Database: PDB / ID: 4nov | ||||||
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Title | Xsa43E, a GH43 family enzyme from Butyrivibrio proteoclasticus | ||||||
![]() | Xylosidase/arabinofuranosidase Xsa43E | ||||||
![]() | HYDROLASE / 5 bladed beta-propellor / arabinofuranosidase | ||||||
Function / homology | ![]() xylan 1,4-beta-xylosidase / xylan 1,4-beta-xylosidase activity / carbohydrate metabolic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Till, M. / Arcus, V.L. | ||||||
![]() | ![]() Title: Structural analysis of the GH43 enzyme Xsa43E from Butyrivibrio proteoclasticus Authors: Till, M. / Goldstone, D. / Card, G. / Attwood, G.T. / Moon, C.D. / Arcus, V.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 79.4 KB | Display | ![]() |
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PDB format | ![]() | 62.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.8 KB | Display | ![]() |
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Full document | ![]() | 445.8 KB | Display | |
Data in XML | ![]() | 16.4 KB | Display | |
Data in CIF | ![]() | 24.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39596.020 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 51982 / DSM 14932 / B316 / Gene: xsa43E, bpr_I2319 / Production host: ![]() ![]() |
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#2: Chemical | ChemComp-CA / |
#3: Chemical | ChemComp-TRS / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.8 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 3.4 Details: 20% PEG8000, 0.2M NaCl, 0.1M Phosphate Citrate buffer , pH 3.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Aug 5, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.33→56.2 Å / Num. all: 86160 / Num. obs: 82628 / % possible obs: 95.9 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2.3 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 16.882 Å2
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Refinement step | Cycle: LAST / Resolution: 1.33→56.08 Å
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Refine LS restraints |
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