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Open data
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Basic information
Entry | Database: PDB / ID: 4no4 | |||||||||
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Title | Crystal Structure of Galectin-1 L11A mutant | |||||||||
![]() | Galectin-1 | |||||||||
![]() | APOPTOSIS / beta barrel / lactose binding protein / lactose | |||||||||
Function / homology | ![]() positive regulation of erythrocyte aggregation / Post-translational protein phosphorylation / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / galectin complex / lactose binding / response to isolation stress / plasma cell differentiation / galactoside binding / negative regulation of cell-substrate adhesion / myoblast differentiation ...positive regulation of erythrocyte aggregation / Post-translational protein phosphorylation / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / galectin complex / lactose binding / response to isolation stress / plasma cell differentiation / galactoside binding / negative regulation of cell-substrate adhesion / myoblast differentiation / cellular response to organic cyclic compound / response to axon injury / laminin binding / T cell costimulation / cellular response to glucose stimulus / cell-cell adhesion / positive regulation of inflammatory response / negative regulation of neuron projection development / carbohydrate binding / collagen-containing extracellular matrix / positive regulation of viral entry into host cell / response to xenobiotic stimulus / positive regulation of apoptotic process / apoptotic process / cell surface / extracellular space / identical protein binding / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Dessau, M. | |||||||||
![]() | ![]() Title: Crystal Structure of Galectin-1 L11A mutant Authors: Dessau, M. / Segev, O. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 477.3 KB | Display | ![]() |
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PDB format | ![]() | 401.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.8 MB | Display | ![]() |
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Full document | ![]() | 2.8 MB | Display | |
Data in XML | ![]() | 48 KB | Display | |
Data in CIF | ![]() | 70.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein / Sugars , 2 types, 12 molecules ABCDEF
#1: Protein | Mass: 14699.545 Da / Num. of mol.: 6 / Mutation: L11A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Polysaccharide | beta-D-galactopyranose-(1-4)-beta-D-glucopyranose / beta-lactose |
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-Non-polymers , 4 types, 1300 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #4: Chemical | #5: Chemical | ChemComp-GOL / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.32 Å3/Da / Density % sol: 62.98 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: PEG 4K, ammonium sulfate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Aug 1, 2006 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→30 Å / Num. obs: 443997 / % possible obs: 98.6 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
Reflection shell | Highest resolution: 1.4 Å / % possible all: 98 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.714 Å2
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Refinement step | Cycle: LAST / Resolution: 1.399→28.969 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 21.1583 Å / Origin y: 29.9525 Å / Origin z: 36.553 Å
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Refinement TLS group | Selection details: all |