+
Open data
-
Basic information
Entry | Database: PDB / ID: 4ncd | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal Structure of Class 5 Fimbriae Chaperone CfaA | ||||||
![]() | Gram-negative pili assembly chaperone, N-terminal domain protein | ||||||
![]() | CHAPERONE / immunoglobulin fold | ||||||
Function / homology | Immunoglobulin-like - #3970 / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / : ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bao, R. / Xia, D. | ||||||
![]() | ![]() Title: Structure of CfaA Suggests a New Family of Chaperones Essential for Assembly of Class 5 Fimbriae. Authors: Bao, R. / Fordyce, A. / Chen, Y.X. / McVeigh, A. / Savarino, S.J. / Xia, D. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 54.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 41.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 28329.703 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.16 % |
---|---|
Crystal grow | Temperature: 293 K / Method: evaporation / pH: 5.3 Details: 22% PEG3350, 0.2 M NaCl, 0.1 M MES pH 5.3, EVAPORATION, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Aug 6, 2010 |
Radiation | Monochromator: APS BM22 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54178 Å / Relative weight: 1 |
Reflection | Resolution: 2.03→50 Å / Num. obs: 15399 / % possible obs: 97.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -1 |
Reflection shell | Resolution: 2.03→2.08 Å / % possible all: 72.9 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.037→39.715 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
|