- PDB-4nbp: Crystal structure of the JCV large T-antigen origin binding domain -
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Basic information
Entry
Database: PDB / ID: 4nbp
Title
Crystal structure of the JCV large T-antigen origin binding domain
Components
Large T antigenLarge tumor antigen
Keywords
HYDROLASE / origin binding domain / DNA replication / binds DNA
Function / homology
Function and homology information
symbiont-mediated suppression of host JAK-STAT cascade via inhibition of JAK1 activity / DNA replication origin binding / symbiont-mediated perturbation of host cell cycle G1/S transition checkpoint / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / DNA replication / hydrolase activity / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / host cell nucleus / ATP binding / metal ion binding Similarity search - Function
Replication Protein E1; Chain: A, - #20 / Large T antigen, polyomaviridae / Replication Protein E1; Chain: A, / Large T antigen, polyomavirus, C-terminal / Zinc finger, large T-antigen D1-type / Origin of replication binding protein / Polyomavirus large T antigen C-terminus / Large T-antigen (T-ag) origin-binding domain (OBD) profile. / Zinc finger large T-antigen (T-ag) D1-type profile. / T antigen, Ori-binding ...Replication Protein E1; Chain: A, - #20 / Large T antigen, polyomaviridae / Replication Protein E1; Chain: A, / Large T antigen, polyomavirus, C-terminal / Zinc finger, large T-antigen D1-type / Origin of replication binding protein / Polyomavirus large T antigen C-terminus / Large T-antigen (T-ag) origin-binding domain (OBD) profile. / Zinc finger large T-antigen (T-ag) D1-type profile. / T antigen, Ori-binding / Zinc finger, large T-antigen D1 domain superfamily / Helicase, superfamily 3, DNA virus / Superfamily 3 helicase of DNA viruses domain profile. / DnaJ molecular chaperone homology domain / dnaJ domain profile. / Chaperone J-domain superfamily / DnaJ domain / P-loop containing nucleoside triphosphate hydrolase / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homology
Mass: 15170.390 Da / Num. of mol.: 1 / Fragment: Origin Binding Domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) JC polyomavirus / Gene: large T antigen / Plasmid: pGEX / Production host: Escherichia coli (E. coli) / Strain (production host): Bl21 References: UniProt: P03072, Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement
Mass: 18.015 Da / Num. of mol.: 222 / Source method: isolated from a natural source / Formula: H2O
Sequence details
THE PRESENCE OF TARTRATE IN THE INDICATED CRYSTALLIZATION CONDITIONS CAUSED THE SIDE CHAIN OF LYS A ...THE PRESENCE OF TARTRATE IN THE INDICATED CRYSTALLIZATION CONDITIONS CAUSED THE SIDE CHAIN OF LYS A 168 TO COVALENTLY BOUND TO TLA A 301. SEE ALSO LINK REMARK
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.58 Å3/Da / Density % sol: 52.39 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 0.19 M sodium tartrate, 19 % Peg 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K
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