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- PDB-4m70: Crystal structure of potato Rx-CC domain in complex with RanGAP2-... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4m70 | ||||||
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Title | Crystal structure of potato Rx-CC domain in complex with RanGAP2-WPP domain | ||||||
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![]() | PLANT PROTEIN / Rx / RanGAP2 / coiled coil domain / WPP domain / plant disease resistance gene / resistance responses / popato X virus | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chai, J. / Hao, W. | ||||||
![]() | ![]() Title: Structural Basis for the Interaction between the Potato Virus X Resistance Protein (Rx) and Its Cofactor Ran GTPase-activating Protein 2 (RanGAP2) Authors: Hao, W. / Collier, S.M. / Moffett, P. / Chai, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 191.1 KB | Display | ![]() |
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PDB format | ![]() | 153.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 505.6 KB | Display | ![]() |
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Full document | ![]() | 526.5 KB | Display | |
Data in XML | ![]() | 33.7 KB | Display | |
Data in CIF | ![]() | 47 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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5 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14272.324 Da / Num. of mol.: 5 / Fragment: Rx-CC domain, UNP residues 1-122 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 11288.781 Da / Num. of mol.: 4 / Fragment: StRanGAP2-WPP domain, UNP residues 15-112 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Protein | | Mass: 9422.042 Da / Num. of mol.: 1 / Fragment: StRanGAP2-WPP domain, UNP residues 15-112 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others), (gene. exp.) ![]() ![]() Gene: RanGAP2, Rx / Plasmid: pGEX-6p-1 / Production host: ![]() ![]() #4: Water | ChemComp-HOH / | Sequence details | THE SEQUENCE OF CHAIN R IS THE SAME OF CHAIN E, J, B, K. THE AUTHOR COULD OBSERVE RESIDUES 23-33, ...THE SEQUENCE OF CHAIN R IS THE SAME OF CHAIN E, J, B, K. THE AUTHOR COULD OBSERVE RESIDUES 23-33, 86-101 AND BELIEVED THAT THESE RESIDUES IS PART OF THE N- TERMINAL RESIDUES 15-37, C-TERMINAL RESIDUES 74-113. BUT THE AUTHOR IS NOT SURE WHICH PART CORRESPOND | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.69 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 10mM Tis, 100 mM NaCl, 39%(V/V) Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 10, 2010 |
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.103→28.648 Å / Num. all: 65379 / Num. obs: 65379 / % possible obs: 100 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Biso Wilson estimate: 42.8 Å2 / Rmerge(I) obs: 0.053 |
Reflection shell | Resolution: 2.1→2.18 Å / % possible all: 97.5 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 120.29 Å2 / Biso mean: 61.7444 Å2 / Biso min: 35.88 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.103→28.648 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 24
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