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Yorodumi- PDB-4m53: Gamma subunit of the translation initiation factor 2 from Sulfolo... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4m53 | |||||||||
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Title | Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDPCP | |||||||||
Components | Translation initiation factor 2 subunit gamma | |||||||||
Keywords | TRANSLATION / aIF2 / aIF2 gamma subunit / G-protein / translation initiation / protein biosynthesis / RNA-binding / GTP-binding / nucleotide-binding | |||||||||
Function / homology | Function and homology information protein-synthesizing GTPase / formation of translation preinitiation complex / translation elongation factor activity / translation initiation factor activity / tRNA binding / GTPase activity / GTP binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Sulfolobus solfataricus (archaea) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | |||||||||
Authors | Nikonov, O.S. / Stolboushkina, E.A. / Arkhipova, V.I. / Lazopulo, A.M. / Lazopulo, S.M. / Gabdulkhakov, A.G. / Nikulin, A.D. / Garber, M.B. / Nikonov, S.V. | |||||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2014 Title: Conformational transitions in the gamma subunit of the archaeal translation initiation factor 2. Authors: Nikonov, O. / Stolboushkina, E. / Arkhipova, V. / Kravchenko, O. / Nikonov, S. / Garber, M. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4m53.cif.gz | 125.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4m53.ent.gz | 93.8 KB | Display | PDB format |
PDBx/mmJSON format | 4m53.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4m53_validation.pdf.gz | 1 MB | Display | wwPDB validaton report |
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Full document | 4m53_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 4m53_validation.xml.gz | 26.5 KB | Display | |
Data in CIF | 4m53_validation.cif.gz | 40.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m5/4m53 ftp://data.pdbj.org/pub/pdb/validation_reports/m5/4m53 | HTTPS FTP |
-Related structure data
Related structure data | 4m0lC 4m2lC 4m4sC 2ahoS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 44563.738 Da / Num. of mol.: 1 / Mutation: 37-47 Deletion mutant Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus solfataricus (archaea) / Strain: P2 / Gene: eif2g / Production host: Escherichia coli (E. coli) / References: UniProt: Q980A5 |
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-Non-polymers , 6 types, 690 molecules
#2: Chemical | ChemComp-BME / | ||||||||
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#3: Chemical | ChemComp-FMT / #4: Chemical | #5: Chemical | ChemComp-MG / | #6: Chemical | ChemComp-NA / #7: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 6.103 Å3/Da / Density % sol: 79.847 % |
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Crystal grow | Temperature: 301 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 25mM Tris-HCl, 400mM NaCl, 10mM 2-mercaptoethanol, 2M sodium formate, 50 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.91841 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Oct 10, 2010 |
Radiation | Monochromator: Si-111 crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91841 Å / Relative weight: 1 |
Reflection | Resolution: 2→19.7 Å / Num. all: 72719 / Num. obs: 72719 / % possible obs: 99.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.01 % / Biso Wilson estimate: 26.961 Å2 / Rmerge(I) obs: 0.1076 / Net I/σ(I): 12.77 |
Reflection shell | Resolution: 2→2.1 Å / Redundancy: 4.9 % / Rmerge(I) obs: 0.4907 / Mean I/σ(I) obs: 2.73 / Num. unique all: 9781 / % possible all: 98.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2AHO Resolution: 2→19.7 Å / Occupancy max: 1 / Occupancy min: 0.18 / SU ML: 0.18 / σ(F): 2 / Phase error: 17.28 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 123.52 Å2 / Biso mean: 25.4363 Å2 / Biso min: 7.02 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→19.7 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 26
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