positive regulation of opsonization / positive regulation of phagocytosis, engulfment / endopeptidase inhibitor activity / immune system process / extracellular space Similarity search - Function
Mass: 150693.594 Da / Num. of mol.: 3 Fragment: Thioester-containing protein I (UNP RESIDUES 22-1338) Source method: isolated from a genetically manipulated source Details: Expression in Bac to Bac System by Invitrogen Source: (gene. exp.) Anopheles gambiae (African malaria mosquito) Strain: PEST / Gene: TEP-I, tep1 / Plasmid: pFastBacI / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q9GYW4
Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 15 Source method: isolated from a genetically manipulated source Formula: C8H15NO6
Identifier
Type
Program
DGlcpNAcb
CONDENSED IUPAC CARBOHYDRATE SYMBOL
GMML 1.0
N-acetyl-b-D-glucopyranosamine
COMMON NAME
GMML 1.0
b-D-GlcpNAc
IUPAC CARBOHYDRATE SYMBOL
PDB-CARE 1.0
GlcNAc
SNFG CARBOHYDRATE SYMBOL
GMML 1.0
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 4.81 Å3/Da / Density % sol: 74.42 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 30% PEG400, 0.1 M Li2SO4, 0.1 M Na citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
Resolution: 3.7→49.12 Å / Cor.coef. Fo:Fc: 0.941 / Cor.coef. Fo:Fc free: 0.926 / Occupancy max: 1 / Occupancy min: 1 / SU B: 23.848 / SU ML: 0.36 / Cross valid method: THROUGHOUT / ESU R Free: 0.124 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.27646
4583
5.1 %
RANDOM
Rwork
0.2368
-
-
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obs
0.23884
86137
99.69 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK