- PDB-4kha: Structural basis of histone H2A-H2B recognition by the essential ... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 4kha
Title
Structural basis of histone H2A-H2B recognition by the essential chaperone FACT
Components
Histone H2A
Spt16M-Histone H2B 1.1 chimera
Keywords
CHAPERONE/NUCLEAR PROTEIN / tandem PHL / Pleckstrin-homology like / U-turn motif / Histone Chaperone / chromatin / transcription / Histones / Nucleus / CHAPERONE-NUCLEAR PROTEIN complex
Function / homology
Function and homology information
FACT complex / nucleosome binding / transcription elongation by RNA polymerase II / structural constituent of chromatin / nucleosome / DNA replication / protein heterodimerization activity / DNA repair / DNA binding / nucleus / metal ion binding Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 116 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.04 Å3/Da / Density % sol: 59.55 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.8 Details: manual setup 1ul protein (15 mg / ml) plus 1 ul crystallization buffer (7.25% [vol/vol] PEG8000, 0.2 M MgCl2, 0.1 M Tris pH 7.8), VAPOR DIFFUSION, HANGING DROP, temperature 277K