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- PDB-4k07: Crystal structure of the amyloid-forming immunoglobulin AL-103 ci... -

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Basic information

Entry
Database: PDB / ID: 4k07
TitleCrystal structure of the amyloid-forming immunoglobulin AL-103 cis-proline 95 mutant
ComponentsAmyloidogenic immunoglobulin light chain protein AL-103
KeywordsIMMUNE SYSTEM / immunoglobulin variable domain / amyloid protein / patient-derived sequence
Function / homology
Function and homology information


CD22 mediated BCR regulation / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement / immunoglobulin complex / FCGR activation / Role of phospholipids in phagocytosis / Role of LAT2/NTAL/LAB on calcium mobilization / Scavenging of heme from plasma / antigen binding ...CD22 mediated BCR regulation / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement / immunoglobulin complex / FCGR activation / Role of phospholipids in phagocytosis / Role of LAT2/NTAL/LAB on calcium mobilization / Scavenging of heme from plasma / antigen binding / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of Complement cascade / Cell surface interactions at the vascular wall / FCGR3A-mediated phagocytosis / FCERI mediated MAPK activation / Regulation of actin dynamics for phagocytic cup formation / FCERI mediated NF-kB activation / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / blood microparticle / Potential therapeutics for SARS / adaptive immune response / immune response / extracellular space / extracellular region / identical protein binding / plasma membrane
Similarity search - Function
Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold ...Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Immunoglobulin kappa variable 1-33
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.83 Å
AuthorsThompson, J.R. / Berkholz, D.S. / Mahlum, E.W. / Ramirez-Alvarado, M.
CitationJournal: J.Mol.Biol. / Year: 2014
Title: Kinetic control in protein folding for light chain amyloidosis and the differential effects of somatic mutations.
Authors: Blancas-Mejia, L.M. / Tischer, A. / Thompson, J.R. / Tai, J. / Wang, L. / Auton, M. / Ramirez-Alvarado, M.
History
DepositionApr 3, 2013Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 30, 2013Provider: repository / Type: Initial release
Revision 1.1Nov 13, 2013Group: Database references
Revision 1.2Jan 22, 2014Group: Database references

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Amyloidogenic immunoglobulin light chain protein AL-103
B: Amyloidogenic immunoglobulin light chain protein AL-103
C: Amyloidogenic immunoglobulin light chain protein AL-103
D: Amyloidogenic immunoglobulin light chain protein AL-103
E: Amyloidogenic immunoglobulin light chain protein AL-103
F: Amyloidogenic immunoglobulin light chain protein AL-103
G: Amyloidogenic immunoglobulin light chain protein AL-103
H: Amyloidogenic immunoglobulin light chain protein AL-103
I: Amyloidogenic immunoglobulin light chain protein AL-103
J: Amyloidogenic immunoglobulin light chain protein AL-103
hetero molecules


Theoretical massNumber of molelcules
Total (without water)140,58411
Polymers140,48810
Non-polymers961
Water59433
1
A: Amyloidogenic immunoglobulin light chain protein AL-103
B: Amyloidogenic immunoglobulin light chain protein AL-103
hetero molecules


Theoretical massNumber of molelcules
Total (without water)28,1943
Polymers28,0982
Non-polymers961
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1430 Å2
ΔGint-12 kcal/mol
Surface area9870 Å2
MethodPISA
2
C: Amyloidogenic immunoglobulin light chain protein AL-103
D: Amyloidogenic immunoglobulin light chain protein AL-103


Theoretical massNumber of molelcules
Total (without water)28,0982
Polymers28,0982
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1390 Å2
ΔGint-12 kcal/mol
Surface area9860 Å2
MethodPISA
3
E: Amyloidogenic immunoglobulin light chain protein AL-103
F: Amyloidogenic immunoglobulin light chain protein AL-103


Theoretical massNumber of molelcules
Total (without water)28,0982
Polymers28,0982
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1520 Å2
ΔGint-20 kcal/mol
Surface area9990 Å2
MethodPISA
4
G: Amyloidogenic immunoglobulin light chain protein AL-103
H: Amyloidogenic immunoglobulin light chain protein AL-103


Theoretical massNumber of molelcules
Total (without water)28,0982
Polymers28,0982
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1410 Å2
ΔGint-12 kcal/mol
Surface area9900 Å2
MethodPISA
5
I: Amyloidogenic immunoglobulin light chain protein AL-103
J: Amyloidogenic immunoglobulin light chain protein AL-103


Theoretical massNumber of molelcules
Total (without water)28,0982
Polymers28,0982
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1380 Å2
ΔGint-12 kcal/mol
Surface area9950 Å2
MethodPISA
Unit cell
Length a, b, c (Å)159.910, 200.334, 40.056
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP21212

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Components

#1: Antibody
Amyloidogenic immunoglobulin light chain protein AL-103


Mass: 14048.806 Da / Num. of mol.: 10 / Fragment: variable domain / Mutation: cis-proline 95 insert
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: P01594*PLUS
#2: Chemical ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 33 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.28 Å3/Da / Density % sol: 46.13 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / pH: 5.5
Details: 15% PEG8000, 443 mM ammonium nitrate, 100 mM sodium acetate, pH 5.5, 666 mM NDSB 201, VAPOR DIFFUSION, HANGING DROP, temperature 291K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-BM / Wavelength: 0.992349 Å
DetectorType: ADSC QUANTUM 210r / Detector: CCD / Date: Oct 17, 2008
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.992349 Å / Relative weight: 1
ReflectionResolution: 2.83→37.13 Å / Num. all: 31753 / Num. obs: 31753 / % possible obs: 98 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.2 % / Rsym value: 0.078 / Net I/σ(I): 21.9
Reflection shellResolution: 2.83→3.048 Å / % possible all: 90

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Processing

Software
NameVersionClassification
HKL-3000data collection
PHENIXmodel building
PHENIX(phenix.refine: dev_1263)refinement
HKL-3000data reduction
HKL-3000data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.83→37.13 Å / SU ML: 0.38 / σ(F): 1.34 / Phase error: 24.69 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2365 642 2.08 %RANDOM
Rwork0.2021 ---
obs0.2028 30878 96.99 %-
all-30878 --
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.83→37.13 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8350 0 5 33 8388
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0068667
X-RAY DIFFRACTIONf_angle_d1.23611815
X-RAY DIFFRACTIONf_dihedral_angle_d14.2023145
X-RAY DIFFRACTIONf_chiral_restr0.0611316
X-RAY DIFFRACTIONf_plane_restr0.0081535
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.83-3.04890.29491220.27165550X-RAY DIFFRACTION91
3.0489-3.35550.28421240.25645950X-RAY DIFFRACTION97
3.3555-3.84070.27491320.21546025X-RAY DIFFRACTION98
3.8407-4.83710.22291310.17676192X-RAY DIFFRACTION99
4.8371-37.13280.2091330.1926519X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
19.13382.0032-0.22635.36260.54196.72920.5616-0.37320.24420.4263-0.59220.38060.142-0.83860.17160.6026-0.03110.10610.8796-0.1780.5111-30.2337-39.9929-1.6416
26.675-2.305-3.65916.66390.34046.00840.2362-0.48990.2383-0.29280.4472-0.4215-0.34140.6051-0.58780.40520.05010.04340.8442-0.1380.5565-22.5812-37.1897-9.1071
36.78870.6883-0.21524.8741-2.5238.2206-0.71660.64261.8787-0.18420.614-0.2393-0.19881.06330.11490.84140.10510.07270.91820.03960.9721-28.4079-30.4144-13.3341
48.46123.0865-2.46083.1669-1.22941.92680.02690.03860.4719-0.30560.1354-0.1317-0.5729-0.2165-0.10830.53620.13570.03170.7859-0.14480.4552-25.643-37.8462-6.903
53.0027-0.60993.43845.5848-2.80334.66540.23920.2593-0.9037-1.2844-0.984-2.15822.84261.32440.18440.8128-0.05360.16051.1026-0.09720.6963-36.5865-48.3428-8.2478
68.5569-0.8642-0.30093.1733-3.60596.8993-0.19870.09920.7912-0.11480.4007-0.1245-0.5172-0.5350.09940.55480.0442-0.00450.6023-0.1260.6188-10.8943-52.4851-19.2117
74.2866-2.7816-2.18262.41690.61926.42020.7810.5979-0.4797-0.845-0.965-0.4609-0.38610.5242-0.2420.42390.1363-0.08690.83620.00130.6212-6.0418-42.372-19.7106
85.7827-0.1689-3.47922.4787-1.00742.316-0.0813-0.4537-0.32441.04970.08530.91820.293-0.79560.30630.5099-0.04740.08790.56710.10.7587-13.7103-48.1055-7.6139
93.0150.75912.18795.6639-3.98695.4353-0.2106-0.43340.39030.8146-0.4771-1.4604-0.329-0.47090.71440.8341-0.0359-0.01710.7929-0.0440.623-2.8861-46.7632-6.7754
106.1079-0.82840.95889.78740.04081.7475-0.2096-0.8940.37470.30740.63230.3197-0.0382-0.397-0.29070.50080.04970.05870.5794-0.03210.2915-10.1632-47.2155-13.7856
113.98190.60043.56629.0221.76174.4654-0.6465-2.5195-0.31230.0477-1.04961.62192.6661-1.11321.01650.9130.03280.13690.9187-0.01840.6689-14.593-61.7452-13.764
123.94210.4682.71484.2375-1.74655.8325-0.3703-0.6703-0.36430.58950.733-0.408-0.0692-1.0509-0.40990.6339-0.0917-0.02940.6552-0.06080.5776-5.2253-66.7201-22.3701
134.2283-5.66723.90318.5918-4.17593.4923-0.537-1.3637-0.06430.51621.3827-0.1346-0.04430.8636-0.68350.7094-0.0892-0.12180.634-0.13520.56285.3797-69.8244-22.6073
144.53993.1864-0.1024.36312.83433.79520.1669-0.20750.1804-0.6325-0.19380.94740.3688-0.96550.34840.6469-0.0754-0.06030.51240.02230.5672-5.0207-72.2778-33.7243
154.1029-6.4229-1.28439.03752.20078.6760.64030.42520.4817-1.1241-0.2963-1.4286-0.5353-0.24040.19070.8670.0611-0.01850.51160.09070.74643.0703-65.0587-35.6236
165.8528-1.42830.61835.9252-0.15414.4624-0.0484-0.0071-0.39320.55130.093-0.0847-0.0177-0.09410.00060.4412-0.02960.03820.2699-0.01540.3403-1.4581-70.064-27.9581
177.4615-0.1237-3.69297.0716-6.85838.7492-1.0922-2.46490.3355-1.5322-0.3187-1.06971.4116-1.0782-0.04120.559-0.0774-0.26550.6431-0.33860.6967-15.0083-63.3011-26.8937
184.6242-0.91210.45334.08732.82866.06230.11720.50850.18-0.52850.18870.24960.5920.1234-0.13650.8050.0671-0.07590.5109-0.10370.7511-8.8927-90.5271-39.9732
194.0947-2.1070.77734.93550.7057.221-0.2583-0.50850.09150.1007-0.157-0.1703-0.28070.48640.02750.8371-0.02190.12090.3664-0.10850.7578-1.946-86.0848-32.5062
205.59655.9404-5.4287.523-7.00846.60830.1302-0.5241-1.8553-0.8253-0.9142-0.75390.66770.86351.00650.8040.0075-0.03420.7285-0.07570.9161-0.1803-94.845-28.4368
217.93741.0803-0.39443.2569-0.8731.9248-0.4014-0.00670.2752-0.380.2271-0.16020.13040.02460.11890.7936-0.08440.00740.4052-0.10490.553-4.3724-88.1174-34.7721
222.62540.59460.70775.6637-5.29545.7784-1.15730.52860.1163-1.03541.48510.61930.7991-1.001-0.67070.8741-0.14880.24290.8662-0.08850.9333-19.3014-90.2366-32.8548
232.63880.9688-0.67798.50814.48554.7883-0.4224-0.00160.0619-1.45220.4929-0.3627-1.26320.5928-0.02060.9014-0.11830.06551.06840.31211.285520.1346-5.0748-30.8266
245.20152.01681.88671.79812.98226.1829-1.21371.4241.9307-0.88411.70071.8436-1.8461-0.1725-0.59140.9740.11480.04330.85520.52761.73739.9329-3.1014-34.912
259.11752.1084-1.19143.61282.97913.62790.5249-1.2551.39170.8451-0.06091.2313-0.2597-0.4924-0.40460.72660.08310.07080.61350.0430.931313.2878-10.4056-21.5842
267.76711.7222-1.52216.6673-3.72033.3551.4266-0.30720.9720.1515-0.81953.90570.21730.1137-0.9881.24840.05430.04370.685-0.13412.11438.3721-0.3706-21.1896
278.5871.43030.82393.45020.87263.4353-0.1768-0.05110.7395-0.43180.43590.9632-0.4345-0.4557-0.08130.9199-0.01650.10730.56430.03731.19613.459-6.6264-27.5804
284.4312-4.63970.93437.0491-4.68736.5465-1.3561-0.531-1.06642.1745-0.8563-1.2617-0.40223.26122.25961.1964-0.04730.0231.04410.41581.252627.5894-6.7045-22.2828
299.3963-3.5799-3.96513.74620.17573.5554-0.1136-1.00180.1251-0.33140.40890.64150.28690.29490.10660.68210.01080.13250.7472-0.03610.728110.8712-29.012-18.9062
308.5489-5.5361-1.899210.04682.75194.1328-0.7751-0.2980.08461.54320.38020.9544-0.0384-0.19490.13840.5602-0.0570.1960.95620.24240.70090.2801-25.4306-19.9287
317.59991.86821.37162.60980.26668.03290.54010.197-0.4758-0.62280.1621-0.37920.03850.6594-0.39120.5762-0.00120.15880.4949-0.06870.678412.0606-22.1535-29.4538
322.8034-3.7599-0.79446.5126-1.0183.09780.61482.2942-1.1562-1.3408-0.77770.79230.2158-1.2463-0.27830.78760.0986-0.04510.8787-0.06940.61673.9157-28.645-33.2092
335.08731.58721.04557.7419-2.04072.2886-0.00570.68640.63570.28930.1730.3523-0.2376-0.6663-0.19820.6178-0.0120.03210.51550.05260.62677.8894-24.8349-24.4523
343.45560.29982.15594.49565.31157.23990.0220.4875-1.0119-0.05890.5814-2.2981-2.67470.92640.07810.7878-0.10650.09570.65140.090.912421.0714-32.0377-22.9227
352.29070.21051.99119.84063.765.87050.15630.41190.5148-1.2472-0.0454-0.0925-1.0229-0.1039-0.20620.6895-0.02690.03440.66220.11250.663315.0821-44.2164-17.2859
366.1288-0.51060.00786.49971.2788.4281-0.2842-0.09860.1730.17560.04520.45580.24950.18920.5470.4994-0.0243-0.08480.5354-0.01070.516216.0367-52.6988-10.272
374.5633-1.5248-2.85314.37043.04963.32960.03910.0349-0.54110.90140.22040.5983-0.0638-0.9365-0.29470.5822-0.07270.05810.7550.05810.68927.226-50.7064-6.2734
384.08121.98740.48263.7946-2.33342.87070.40940.2775-0.2519-0.0195-0.328-0.49780.0725-0.0094-0.0470.5480.0353-0.06580.6039-0.02050.527315.2605-49.4668-12.3804
396.6634-4.7857-5.0673.66123.79093.9722-1.04180.1693-0.16941.40561.2826-0.5020.1782-0.0734-0.16650.86380.0483-0.1180.3988-0.1060.742619.5537-35.0953-9.897
403.33741.6336-0.12516.2276-1.80538.53630.6595-0.3987-0.95960.3887-0.3643-0.0879-0.24160.3093-0.32950.63050.1087-0.03270.6166-0.02720.67431.7724-61.5718-0.2319
419.5641-1.50672.20816.30981.17437.42620.36981.135-0.5103-0.1753-0.14070.5005-0.21310.494-0.20020.37760.02150.04030.70960.01760.649229.471-56.2144-11.6661
425.72944.0415-0.71393.9106-2.55514.4045-1.81780.8757-1.5467-1.46631.1475-0.57261.1333-0.39920.9350.98910.0893-0.10241.2187-0.33261.177732.7646-66.7894-13.3469
433.67281.1962-0.5177.9644-0.52165.3930.3226-0.3872-0.3527-0.0291-0.01970.16720.75220.6952-0.17740.63990.1082-0.1890.63110.03280.565630.0287-60.1857-5.9319
442.02755.1284-0.49234.54831.53363.7614-0.4969-3.58942.9519-0.6685-0.87621.0448-1.53770.26191.13550.6091-0.0632-0.30560.7740.08791.002141.5231-50.3703-5.3334
453.9504-0.787-0.0872.87434.85346.71390.2134-0.44210.60421.0225-0.3268-0.08080.2556-0.38370.13060.8046-0.04070.00710.6723-0.0050.886819.9947-94.8736-1.8962
463.0082-3.0652.76143.7989-3.93688.15540.50261.2062-0.68050.4794-0.64070.13510.3070.83650.15930.708-0.0613-0.03550.6667-0.07160.662827.511-97.3778-12.0695
478.2448-3.772-4.01156.60964.59139.4161-0.46370.19130.7575-0.6985-0.01350.25980.4711-0.33070.40971.1117-0.0808-0.00220.5273-0.01840.854518.1019-92.9557-15.7749
485.2421-0.6396-2.12269.21542.48616.41690.1371-0.2924-0.1423-0.68040.0937-0.3968-0.17310.4254-0.06940.53390.01260.08780.6933-0.03040.489223.1241-94.9082-7.0432
493.68163.1923-0.07074.6096-2.23413.4760.7601-1.4188-0.8237-1.5283-2.5996-2.14440.10860.6121.43190.8161-0.24420.07051.00230.19960.946622.4774-109.9895-5.6548
501.3642-1.0065-1.52383.76212.41552.0776-0.16240.865-0.4148-0.5581-0.2553-1.21960.74031.15210.37720.98790.03010.36981.15630.01111.535646.1356-96.2998-13.2676
515.3363-2.0830.16357.69467.33241.91081.59971.9530.9083-1.1694-1.00053.0675-7.6647-0.3359-2.05661.0142-0.39130.93771.80940.18252.41543.3333-86.0427-16.766
525.79953.2096-5.79083.7292-1.37777.59250.3333-0.27532.50810.66660.82481.6578-0.34770.2129-0.84440.7990.03340.04611.04140.00511.530538.3391-93.1323-3.8404
536.8373-4.668-2.4276.8772-0.6061.8803-0.43190.07641.8538-0.1001-0.1039-2.7120.0484-1.4371-0.28761.0202-0.3194-0.06761.9785-0.74422.101944.928-84.1154-2.9001
545.3246-3.6096-5.69834.96371.07698.450.85011.32631.84190.70370.34030.5991-1.54660.5418-1.43480.9703-0.09930.21561.56650.14491.627141.8482-91.2048-9.6838
552.00782.00411.99731.97181.95462.0206-3.367-1.26951.00325.6032-1.3933-0.38480.1475-0.43561.28541.50130.08140.53840.89370.24411.046948.0947-104.2996-5.2153
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 0 through 25 )
2X-RAY DIFFRACTION2chain 'A' and (resid 26 through 48 )
3X-RAY DIFFRACTION3chain 'A' and (resid 49 through 67 )
4X-RAY DIFFRACTION4chain 'A' and (resid 68 through 101 )
5X-RAY DIFFRACTION5chain 'A' and (resid 102 through 107 )
6X-RAY DIFFRACTION6chain 'B' and (resid 0 through 18 )
7X-RAY DIFFRACTION7chain 'B' and (resid 19 through 32 )
8X-RAY DIFFRACTION8chain 'B' and (resid 33 through 48 )
9X-RAY DIFFRACTION9chain 'B' and (resid 49 through 67 )
10X-RAY DIFFRACTION10chain 'B' and (resid 68 through 101 )
11X-RAY DIFFRACTION11chain 'B' and (resid 102 through 107 )
12X-RAY DIFFRACTION12chain 'C' and (resid 0 through 18 )
13X-RAY DIFFRACTION13chain 'C' and (resid 19 through 32 )
14X-RAY DIFFRACTION14chain 'C' and (resid 33 through 48 )
15X-RAY DIFFRACTION15chain 'C' and (resid 49 through 67 )
16X-RAY DIFFRACTION16chain 'C' and (resid 68 through 101 )
17X-RAY DIFFRACTION17chain 'C' and (resid 102 through 107 )
18X-RAY DIFFRACTION18chain 'D' and (resid 0 through 25 )
19X-RAY DIFFRACTION19chain 'D' and (resid 26 through 48 )
20X-RAY DIFFRACTION20chain 'D' and (resid 49 through 67 )
21X-RAY DIFFRACTION21chain 'D' and (resid 68 through 101 )
22X-RAY DIFFRACTION22chain 'D' and (resid 102 through 107 )
23X-RAY DIFFRACTION23chain 'E' and (resid 0 through 18 )
24X-RAY DIFFRACTION24chain 'E' and (resid 19 through 32 )
25X-RAY DIFFRACTION25chain 'E' and (resid 33 through 48 )
26X-RAY DIFFRACTION26chain 'E' and (resid 49 through 67 )
27X-RAY DIFFRACTION27chain 'E' and (resid 68 through 101 )
28X-RAY DIFFRACTION28chain 'E' and (resid 102 through 107 )
29X-RAY DIFFRACTION29chain 'F' and (resid 0 through 18 )
30X-RAY DIFFRACTION30chain 'F' and (resid 19 through 32 )
31X-RAY DIFFRACTION31chain 'F' and (resid 33 through 48 )
32X-RAY DIFFRACTION32chain 'F' and (resid 49 through 67 )
33X-RAY DIFFRACTION33chain 'F' and (resid 68 through 101 )
34X-RAY DIFFRACTION34chain 'F' and (resid 102 through 107 )
35X-RAY DIFFRACTION35chain 'G' and (resid 0 through 25 )
36X-RAY DIFFRACTION36chain 'G' and (resid 26 through 48 )
37X-RAY DIFFRACTION37chain 'G' and (resid 49 through 67 )
38X-RAY DIFFRACTION38chain 'G' and (resid 68 through 101 )
39X-RAY DIFFRACTION39chain 'G' and (resid 102 through 107 )
40X-RAY DIFFRACTION40chain 'H' and (resid 0 through 32 )
41X-RAY DIFFRACTION41chain 'H' and (resid 33 through 48 )
42X-RAY DIFFRACTION42chain 'H' and (resid 49 through 67 )
43X-RAY DIFFRACTION43chain 'H' and (resid 68 through 101 )
44X-RAY DIFFRACTION44chain 'H' and (resid 102 through 107 )
45X-RAY DIFFRACTION45chain 'I' and (resid 0 through 32 )
46X-RAY DIFFRACTION46chain 'I' and (resid 33 through 48 )
47X-RAY DIFFRACTION47chain 'I' and (resid 49 through 67 )
48X-RAY DIFFRACTION48chain 'I' and (resid 68 through 101 )
49X-RAY DIFFRACTION49chain 'I' and (resid 102 through 107 )
50X-RAY DIFFRACTION50chain 'J' and (resid 0 through 18 )
51X-RAY DIFFRACTION51chain 'J' and (resid 19 through 32 )
52X-RAY DIFFRACTION52chain 'J' and (resid 33 through 48 )
53X-RAY DIFFRACTION53chain 'J' and (resid 49 through 67 )
54X-RAY DIFFRACTION54chain 'J' and (resid 68 through 101 )
55X-RAY DIFFRACTION55chain 'J' and (resid 102 through 107 )

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