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- PDB-4jw2: Selection of specific protein binders for pre-defined targets fro... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4jw2 | ||||||
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Title | Selection of specific protein binders for pre-defined targets from an optimized library of artificial helicoidal repeat proteins (alphaRep) | ||||||
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![]() | DE NOVO PROTEIN/protein binding / alpha-helical proteins / Proteins engineered to bind to various partners / DE NOVO PROTEIN-protein binding complex | ||||||
Function / homology | Leucine-rich Repeat Variant / Leucine-rich Repeat Variant / Alpha Horseshoe / Mainly Alpha![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Guellouz, A. / Valerio-Lepiniec, M. / Urvoas, A. / Chevrel, A. / Graille, M. / Fourati-Kammoun, Z. / Desmadril, M. / van Tilbeurgh, H. / Minard, P. | ||||||
![]() | ![]() Title: Selection of Specific Protein Binders for Pre-Defined Targets from an Optimized Library of Artificial Helicoidal Repeat Proteins (alphaRep). Authors: Guellouz, A. / Valerio-Lepiniec, M. / Urvoas, A. / Chevrel, A. / Graille, M. / Fourati-Kammoun, Z. / Desmadril, M. / van Tilbeurgh, H. / Minard, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 66.3 KB | Display | ![]() |
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PDB format | ![]() | 48.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 446.4 KB | Display | ![]() |
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Full document | ![]() | 446.9 KB | Display | |
Data in XML | ![]() | 12.5 KB | Display | |
Data in CIF | ![]() | 17.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4jw3C ![]() 3ltmS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11947.639 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Plasmid: pQE81L / Production host: ![]() ![]() | ||
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#2: Protein | Mass: 22224.102 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Plasmid: pQE81L / Production host: ![]() ![]() | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.01 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.6 Details: 8% (w/v) PEG 2,000 MME, 100 mM Na acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Feb 29, 2012 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→30 Å / Num. all: 24720 / Num. obs: 24498 / % possible obs: 99.1 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 5.5 % / Biso Wilson estimate: 36.05 Å2 / Rsym value: 0.048 / Net I/σ(I): 17.6 |
Reflection shell | Resolution: 1.9→1.95 Å / Redundancy: 5.5 % / Mean I/σ(I) obs: 2 / Rsym value: 0.566 / % possible all: 98.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3LTM Resolution: 1.9→28.83 Å / Cor.coef. Fo:Fc: 0.9204 / Cor.coef. Fo:Fc free: 0.9132 / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 48.27 Å2
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Refine analyze | Luzzati coordinate error obs: 0.261 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→28.83 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→1.98 Å / Total num. of bins used: 12
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