Insect allergen-related / Insect allergen related repeat, nitrile-specifier detoxification / Green Fluorescent Protein / Green fluorescent protein / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / Beta Barrel / Mainly Beta Similarity search - Domain/homology
Mass: 46738.820 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: designed off of pET28a to include a 6HIS-EGFP fusion at the N-terminus with a AAA linker region in between the EGFP and the Blag1 Source: (gene. exp.) Aequorea victoria (jellyfish), (gene. exp.) Blattella germanica (German cockroach) Gene: GFP / Plasmid: pEGFPX / Production host: Escherichia coli (E. coli) / Strain (production host): BL21DE3-RIL / References: UniProt: P42212, UniProt: Q9UAM5
Mass: 18.015 Da / Num. of mol.: 100 / Source method: isolated from a natural source / Formula: H2O
Nonpolymer details
THE LIGAND DENOTED ADS D12 IS AN UNKNOWN FATTY ACID, WITH THE CLOSEST MATCH ON OBSERVED ATOMS TO ...THE LIGAND DENOTED ADS D12 IS AN UNKNOWN FATTY ACID, WITH THE CLOSEST MATCH ON OBSERVED ATOMS TO DODECANE. THE LIGAND DENOTED AS PGT IS AN UNKNOWN PHOSPHOLIPID. OTHER STUDIES SUGGEST IT IS LIKELY A PHOSPHATIDYL GLYCEROL FROM BACTERIA
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.26 Å3/Da / Density % sol: 62.22 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 100mM Tris, 10% propanol, 20% PEG4K, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
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