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- PDB-4jlf: Inhibitor resistant (R220A) substitution in the Mycobacterium tub... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4jlf | ||||||
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Title | Inhibitor resistant (R220A) substitution in the Mycobacterium tuberculosis beta-lactamase | ||||||
![]() | Beta-lactamase | ||||||
![]() | HYDROLASE / beta-lactams inactivation / tuberculosis / Drug Design / Multi drug resistance | ||||||
Function / homology | ![]() : / : / beta-lactam antibiotic catabolic process / beta-lactamase activity / beta-lactamase / periplasmic space / response to antibiotic / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hazra, S. / Kurz, S. / Blanchard, J. / Bonomo, R. | ||||||
![]() | ![]() Title: Can inhibitor-resistant substitutions in the Mycobacterium tuberculosis beta-Lactamase BlaC lead to clavulanate resistance?: a biochemical rationale for the use of beta-lactam-beta-lactamase ...Title: Can inhibitor-resistant substitutions in the Mycobacterium tuberculosis beta-Lactamase BlaC lead to clavulanate resistance?: a biochemical rationale for the use of beta-lactam-beta-lactamase inhibitor combinations. Authors: Kurz, S.G. / Wolff, K.A. / Hazra, S. / Bethel, C.R. / Hujer, A.M. / Smith, K.M. / Xu, Y. / Tremblay, L.W. / Blanchard, J.S. / Nguyen, L. / Bonomo, R.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 70.6 KB | Display | ![]() |
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PDB format | ![]() | 50.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 423 KB | Display | ![]() |
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Full document | ![]() | 423.1 KB | Display | |
Data in XML | ![]() | 14 KB | Display | |
Data in CIF | ![]() | 20.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2gdnS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 28186.607 Da / Num. of mol.: 1 / Fragment: beta lactamase (blaC) / Mutation: R220A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P0C5C1, UniProt: P9WKD3*PLUS, beta-lactamase | ||
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#2: Chemical | ChemComp-PO4 / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.23 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 0.1 M HEPES, 2 M NH4H2PO4, pH 7.5, Vapor diffusion, Sitting drop, temperature 298K, VAPOR DIFFUSION, SITTING DROP |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD |
Radiation | Monochromator: Si(111) Channel Cut / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→100 Å / Num. all: 15203 / Num. obs: 15203 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 2.1→2.14 Å / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2GDN Resolution: 2.1→37.3 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.1→37.3 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.14 Å |