enzyme regulator activity / bioluminescence / generation of precursor metabolites and energy / calcium ion binding 類似検索 - 分子機能
Green Fluorescent Protein / Green fluorescent protein / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. ...Green Fluorescent Protein / Green fluorescent protein / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Beta Barrel / Mainly Beta 類似検索 - ドメイン・相同性
(1) THIS CHIMERA COMPRISES OF RESIDUES 25-48 OF RCaMP, LINKER LE, RESIDUES 149-238 OF GREEN ...(1) THIS CHIMERA COMPRISES OF RESIDUES 25-48 OF RCaMP, LINKER LE, RESIDUES 149-238 OF GREEN FLUORESCENT PROTEIN, LINKER GGTGGSMV, RESIDUES 2-144 OF GREEN FLUORESCENT PROTEIN, RESIDUES 284-432 OF RCaMP (2) RESIDUE SER 222 HAS BEEN MUTATED TO GLY. RESIDUES GLY 222, TYR 223 AND GLY 224 CONSTITUTE THE CHROMOPHORE CRO 222
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 3.73 Å3/Da / 溶媒含有率: 67.03 %
結晶化
温度: 289 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8.5 詳細: Tris pH 8.5, NH4OAc, 23% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: 回転陽極 / タイプ: RIGAKU MICROMAX-007 / 波長: 1.5418 Å
構造決定の手法: 分子置換 / 解像度: 2.5→46.95 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.924 / SU B: 6.655 / SU ML: 0.15 / 交差検証法: THROUGHOUT / ESU R: 0.266 / ESU R Free: 0.222 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.22797
1244
5 %
RANDOM
Rwork
0.17586
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all
0.1843
23790
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obs
0.17846
23529
98.9 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK