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Structure paper

TitleStructural insight into enhanced calcium indicator GCaMP3 and GCaMPJ to promote further improvement.
Journal, issue, pagesProtein Cell, Vol. 4, Page 299-309, Year 2013
Publish dateDec 25, 2012 (structure data deposition date)
AuthorsChen, Y. / Song, X. / Ye, S. / Miao, L. / Zhu, Y. / Zhang, R.G. / Ji, G.
External linksProtein Cell / PubMed:23549615
MethodsX-ray diffraction
Resolution1.55 - 2.5 Å
Structure data

PDB-4ik1:
High resolution structure of GCaMPJ at pH 8.5
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4ik3:
High resolution structure of GCaMP3 at pH 8.5
Method: X-RAY DIFFRACTION / Resolution: 2.007 Å

PDB-4ik4:
High resolution structure of GCaMP3 at pH 5.0
Method: X-RAY DIFFRACTION / Resolution: 2.01 Å

PDB-4ik5:
High resolution structure of Delta-REST-GCaMP3
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-4ik8:
High resolution structure of GCaMP3 dimer form 1 at pH 7.5
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

PDB-4ik9:
High resolution structure of GCaMP3 dimer form 2 at pH 7.5
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • entacmaea quadricolor (sea anemone)
  • aequorea victoria (jellyfish)
KeywordsFLUORESCENT PROTEIN / calcium indicator / fluorescent intensity / dimerization / beta barrel / calmodulin / mutants / beta-barrel / Ca ion / Cytpplasm

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