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- PDB-4h82: Crystal structure of mutant MMP-9 catalytic domain in complex wit... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4h82 | ||||||
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Title | Crystal structure of mutant MMP-9 catalytic domain in complex with a twin inhibitor. | ||||||
![]() | Matrix metalloproteinase-9 | ||||||
![]() | hydrolase/hydrolase inhibitor / HYDROLASE/TWIN INHIBITOR / Zincin-like / Gelatinase / Collagenase (Catalytic Domain) / hydrolase-hydrolase inhibitor complex | ||||||
Function / homology | ![]() gelatinase B / negative regulation of epithelial cell differentiation involved in kidney development / negative regulation of cation channel activity / negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway / cellular response to UV-A / positive regulation of keratinocyte migration / regulation of neuroinflammatory response / positive regulation of epidermal growth factor receptor signaling pathway / Assembly of collagen fibrils and other multimeric structures / Activation of Matrix Metalloproteinases ...gelatinase B / negative regulation of epithelial cell differentiation involved in kidney development / negative regulation of cation channel activity / negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway / cellular response to UV-A / positive regulation of keratinocyte migration / regulation of neuroinflammatory response / positive regulation of epidermal growth factor receptor signaling pathway / Assembly of collagen fibrils and other multimeric structures / Activation of Matrix Metalloproteinases / endodermal cell differentiation / positive regulation of release of cytochrome c from mitochondria / response to amyloid-beta / macrophage differentiation / Collagen degradation / collagen catabolic process / EPH-ephrin mediated repulsion of cells / extracellular matrix disassembly / ephrin receptor signaling pathway / positive regulation of DNA binding / negative regulation of intrinsic apoptotic signaling pathway / positive regulation of vascular associated smooth muscle cell proliferation / collagen binding / cellular response to cadmium ion / embryo implantation / Degradation of the extracellular matrix / extracellular matrix organization / positive regulation of receptor binding / skeletal system development / Signaling by SCF-KIT / metalloendopeptidase activity / cellular response to reactive oxygen species / metallopeptidase activity / cell migration / tertiary granule lumen / peptidase activity / cellular response to lipopolysaccharide / Interleukin-4 and Interleukin-13 signaling / collagen-containing extracellular matrix / endopeptidase activity / ficolin-1-rich granule lumen / Extra-nuclear estrogen signaling / positive regulation of protein phosphorylation / positive regulation of apoptotic process / serine-type endopeptidase activity / apoptotic process / Neutrophil degranulation / negative regulation of apoptotic process / proteolysis / extracellular space / zinc ion binding / extracellular exosome / extracellular region / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Antoni, C. / Stura, E.A. / Vera, L. / Cassar-Lajeunesse, E. / Nuti, E. / Dive, V. / Rossello, A. | ||||||
![]() | ![]() Title: Crystallization of bi-functional ligand protein complexes. Authors: Antoni, C. / Vera, L. / Devel, L. / Catalani, M.P. / Czarny, B. / Cassar-Lajeunesse, E. / Nuti, E. / Rossello, A. / Dive, V. / Stura, E.A. #1: Journal: Bioorg.Med.Chem.Lett. / Year: 2005 Title: A new development of matrix metalloproteinase inhibitors: twin hydroxamic acids as potent inhibitors of MMPs. Authors: Rossello, A. / Nuti, E. / Catalani, M.P. / Carelli, P. / Orlandini, E. / Rapposelli, S. / Tuccinardi, T. / Atkinson, S.J. / Murphy, G. / Balsamo, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 170.8 KB | Display | ![]() |
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PDB format | ![]() | 133.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 38.9 KB | Display | |
Data in CIF | ![]() | 55.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4h1qSC ![]() 4h2eC ![]() 4h30C ![]() 4h3xC ![]() 4h49C ![]() 4h76C ![]() 4h84C ![]() 4hmaC ![]() 4i03C C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The assembly in the asymetric unit are two dimers. Its biological significance is debatable. |
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Components
-Protein , 1 types, 4 molecules ABCD
#1: Protein | Mass: 17932.871 Da / Num. of mol.: 4 / Fragment: MMP-9 catalytic domain 107-215,391-444 / Mutation: V391Q E402Q Source method: isolated from a genetically manipulated source Details: construct: 110 216 and 392 444 Mutagenesis: Glu402Gln Structure: renumbered omi tting missing domain. Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 8 types, 738 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/L29.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/PPI.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/PGO.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/L29.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/PPI.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/PGO.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-CA / #4: Chemical | #5: Chemical | ChemComp-PEG / #6: Chemical | ChemComp-PPI / | #7: Chemical | ChemComp-GOL / | #8: Chemical | #9: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.83 % |
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Crystal grow | Temperature: 293 K / pH: 8 Details: Protein: MMP9h Nter=GFQT E402Q V391Q at 384.3 micro-M with 120 milli-M AHA Reservoir: 10% MPEG 20K, 100mM PCTP 75/25, 1.5 NaCl. Cryoprotectant: 12.5% di-ethylene glycol, 12.5% DMSO, 12.5% ...Details: Protein: MMP9h Nter=GFQT E402Q V391Q at 384.3 micro-M with 120 milli-M AHA Reservoir: 10% MPEG 20K, 100mM PCTP 75/25, 1.5 NaCl. Cryoprotectant: 12.5% di-ethylene glycol, 12.5% DMSO, 12.5% MPD, 12.5% 1,2-propnaediol, 25% glycerol, 9% PEG 10K, 100 milli-M PCTP 80/20, 1.5 NaCl , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 4, 2012 / Details: MIRRORS |
Radiation | Monochromator: SI 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98011 Å / Relative weight: 1 |
Reflection twin | Operator: -h,k,-l / Fraction: 0.48 |
Reflection | Resolution: 1.9→50 Å / Num. obs: 45622 / % possible obs: 86.8 % / Observed criterion σ(I): -3 / Redundancy: 2.25 % / Biso Wilson estimate: 29.73 Å2 / Rmerge(I) obs: 0.132 / Rsym value: 0.104 / Net I/σ(I): 6.35 |
Reflection shell | Resolution: 1.9→2.02 Å / Redundancy: 2.17 % / Rmerge(I) obs: 0.773 / Mean I/σ(I) obs: 1.21 / Rsym value: 0.694 / % possible all: 68.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4H1Q Resolution: 1.9→33.46 Å / Isotropic thermal model: Isotropic / σ(F): 1.99 / Phase error: 24.84 / Stereochemistry target values: TWIN_LSQ_F
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.98 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→33.46 Å
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Refine LS restraints |
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LS refinement shell |
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