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- PDB-4gzz: Crystal structures of bacterial RNA Polymerase paused elongation ... -

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Basic information

Entry
Database: PDB / ID: 4gzz
TitleCrystal structures of bacterial RNA Polymerase paused elongation complexes
Components
  • (DNA-directed RNA polymerase subunit ...Polymerase) x 4
  • RNA transcriptTranscription (biology)
  • non-template DNA
  • template DNA
KeywordsTRANSCRIPTION/DNA-RNA HYBRID / RNA Polymerase / transcription / paused transcription elongation complex / transcriptional pausing / DNA directed RNA transcription' / TRANSCRIPTION-DNA-RNA HYBRID complex
Function / homology
Function and homology information


DNA-directed RNA polymerase complex / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding / zinc ion binding / cytoplasm
Similarity search - Function
: / DNA-directed RNA polymerase subunit beta', hybrid domain / DNA-directed RNA polymerase, omega subunit / DNA-directed RNA polymerase, subunit beta-prime, bacterial type / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase, alpha subunit ...: / DNA-directed RNA polymerase subunit beta', hybrid domain / DNA-directed RNA polymerase, omega subunit / DNA-directed RNA polymerase, subunit beta-prime, bacterial type / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase, alpha subunit / DNA-directed RNA polymerase beta subunit, bacterial-type / RNA polymerase Rpb6 / RNA polymerase, subunit omega/Rpo6/RPB6 / RNA polymerase Rpb6 / RNA polymerase Rpb1, domain 3 superfamily / RNA polymerase Rpb1, clamp domain superfamily / RPB6/omega subunit-like superfamily / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 4 / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 5 / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / DNA-directed RNA polymerase, insert domain / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / RNA polymerase Rpb3/RpoA insert domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerases D / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase, RBP11-like subunit / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6
Similarity search - Domain/homology
DNA / DNA (> 10) / RNA / RNA (> 10) / DNA-directed RNA polymerase subunit alpha / DNA-directed RNA polymerase subunit omega / DNA-directed RNA polymerase subunit beta' / DNA-directed RNA polymerase subunit beta
Similarity search - Component
Biological speciesThermus thermophilus (bacteria)
synthetic (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 4.2927 Å
AuthorsWeixlbaumer, A. / Leon, K. / Landick, R. / Darst, S.A.
CitationJournal: Cell(Cambridge,Mass.) / Year: 2013
Title: Structural basis of transcriptional pausing in bacteria.
Authors: Weixlbaumer, A. / Leon, K. / Landick, R. / Darst, S.A.
History
DepositionSep 6, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 13, 2013Provider: repository / Type: Initial release
Revision 1.1Nov 15, 2017Group: Refinement description / Category: software
Item: _software.classification / _software.contact_author ..._software.classification / _software.contact_author / _software.contact_author_email / _software.date / _software.language / _software.location / _software.name / _software.type / _software.version
Revision 1.2Jul 17, 2019Group: Data collection / Refinement description / Category: software
Item: _software.classification / _software.contact_author ..._software.classification / _software.contact_author / _software.contact_author_email / _software.language / _software.location / _software.name / _software.version
Revision 1.3Sep 13, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA-directed RNA polymerase subunit alpha
B: DNA-directed RNA polymerase subunit alpha
C: DNA-directed RNA polymerase subunit beta
D: DNA-directed RNA polymerase subunit beta'
E: DNA-directed RNA polymerase subunit omega
N: non-template DNA
R: RNA transcript
T: template DNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)395,44411
Polymers395,2898
Non-polymers1553
Water0
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area37460 Å2
ΔGint-196 kcal/mol
Surface area128850 Å2
MethodPISA
Unit cell
Length a, b, c (Å)286.549, 286.549, 199.411
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number146
Space group name H-MH3

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Components

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DNA-directed RNA polymerase subunit ... , 4 types, 5 molecules ABCDE

#1: Protein DNA-directed RNA polymerase subunit alpha / Polymerase / RNAP subunit alpha / RNA polymerase subunit alpha / Transcriptase subunit alpha


Mass: 35056.164 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Gene: rpoA, TTHA1664 / Production host: Thermus thermophilus (bacteria) / Strain (production host): HB8 / References: UniProt: Q5SHR6, DNA-directed RNA polymerase
#2: Protein DNA-directed RNA polymerase subunit beta / Polymerase / RNAP subunit beta / RNA polymerase subunit beta / Transcriptase subunit beta


Mass: 125436.539 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Gene: rpoB, TTHA1813 / Production host: Thermus thermophilus (bacteria) / Strain (production host): HB8 / References: UniProt: Q8RQE9, DNA-directed RNA polymerase
#3: Protein DNA-directed RNA polymerase subunit beta' / Polymerase / RNAP subunit beta' / RNA polymerase subunit beta' / Transcriptase subunit beta'


Mass: 172378.812 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: genomic copy of rpoC replaced by a C-terminal 10His tagged version
Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Gene: rpoC, TTHA1812 / Production host: Thermus thermophilus (bacteria) / Strain (production host): HB8 / References: UniProt: Q8RQE8, DNA-directed RNA polymerase
#4: Protein DNA-directed RNA polymerase subunit omega / Polymerase / RNAP omega subunit / RNA polymerase omega subunit / Transcriptase subunit omega


Mass: 11533.316 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Gene: rpoZ, TTHA1561 / Production host: Thermus thermophilus (bacteria) / Strain (production host): HB8 / References: UniProt: Q8RQE7, DNA-directed RNA polymerase

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DNA chain , 2 types, 2 molecules NT

#5: DNA chain non-template DNA


Mass: 4000.624 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic (others)
#7: DNA chain template DNA


Mass: 6696.343 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic (others)

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RNA chain , 1 types, 1 molecules R

#6: RNA chain RNA transcript / Transcription (biology)


Mass: 5131.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic (others)

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Non-polymers , 2 types, 3 molecules

#8: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#9: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.99 Å3/Da / Density % sol: 69.14 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.2
Details: 5-7% PEG 3000, 0.1M MES pH 6.1-6.3, 0.3M LiCl, 10mM MgCl2, vapor diffusion, hanging drop, temperature 295K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.91956 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Nov 4, 2011
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.91956 Å / Relative weight: 1
ReflectionResolution: 4→45 Å / Num. all: 52138 / Num. obs: 51877 / % possible obs: 99.5 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 5.9 % / Rmerge(I) obs: 0.442 / Net I/σ(I): 4.9
Reflection shell
Resolution (Å)Redundancy (%)Diffraction-ID% possible allRmerge(I) obs
4-4.074.8197.7
4.07-4.145.9199.7
4.14-4.226199.5
4.22-4.316199.4
4.31-4.46199.5
4.4-4.56199.5
4.5-4.626199.6
4.62-4.746199.4
4.74-4.886199.4
4.88-5.046199.70.947
5.04-5.226199.70.859
5.22-5.436199.50.806
5.43-5.676199.80.751
5.67-5.976199.70.663
5.97-6.355.9199.70.565
6.35-6.835.9199.80.495
6.83-7.525.8199.70.451
7.52-8.65.7199.80.43
8.6-10.815.6199.70.887
10.81-455.9199.80.719

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Phasing

PhasingMethod: molecular replacement
Phasing MRMethod rotation: fast direct / Method translation: &STRIP%trans_method

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Processing

Software
NameVersionClassificationNB
PHENIX1.8_1069refinement
SCALEPACKdata scaling
CNSrefinement
DENZOdata reduction
PDB_EXTRACT3.11data extraction
HKL-2000data collection
CNSphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 2O5I

2o5i


Resolution: 4.2927→38.785 Å / Occupancy max: 1 / Occupancy min: 1 / SU ML: 0.67 / σ(F): 1.96 / Phase error: 31.52 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2849 2070 5.02 %
Rwork0.2345 --
obs0.237 41266 99.01 %
all-41681 -
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 125.9191 Å2
Refinement stepCycle: LAST / Resolution: 4.2927→38.785 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms23534 863 3 0 24400
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00524969
X-RAY DIFFRACTIONf_angle_d0.93433909
X-RAY DIFFRACTIONf_dihedral_angle_d16.3439678
X-RAY DIFFRACTIONf_chiral_restr0.0613835
X-RAY DIFFRACTIONf_plane_restr0.0054309
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
4.2927-4.39250.3641500.34632516X-RAY DIFFRACTION95
4.3925-4.50220.32911530.33172579X-RAY DIFFRACTION99
4.5022-4.62370.3351170.30422639X-RAY DIFFRACTION99
4.6237-4.75950.37251640.28612585X-RAY DIFFRACTION99
4.7595-4.91290.29511460.28452624X-RAY DIFFRACTION99
4.9129-5.08810.29781350.27362641X-RAY DIFFRACTION100
5.0881-5.29130.28881240.27612622X-RAY DIFFRACTION99
5.2913-5.53150.33721350.27462626X-RAY DIFFRACTION100
5.5315-5.82220.36691330.27162639X-RAY DIFFRACTION99
5.8222-6.18560.30491460.26032624X-RAY DIFFRACTION100
6.1856-6.6610.30881510.24182618X-RAY DIFFRACTION100
6.661-7.32730.27441430.21452649X-RAY DIFFRACTION100
7.3273-8.37820.25511260.18932598X-RAY DIFFRACTION99
8.3782-10.52070.23511240.18022648X-RAY DIFFRACTION99
10.5207-38.78670.25131230.21422588X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.5699-0.44810.2751.2443-0.52461.6987-0.760.5218-0.81940.05550.17040.79520.2705-0.728-1.74980.3774-0.13410.12190.10510.70280.4208-166.498418.021868.3318
21.2254-0.19070.42921.95270.25611.3096-0.5483-0.19680.64320.08930.5629-0.6373-0.59280.43811.63371.17160.0973-0.61490.46520.40430.2829-131.819141.220282.4732
30.35120.13620.03220.5329-0.16910.1259-0.2261-0.38080.43090.45470.11790.079-0.5406-0.0105-0.02481.3647-0.0659-0.86551.05610.16990.9259-107.393328.5831111.8765
40.0384-0.0512-0.03910.12060.030.1118-0.496-0.02210.36980.67680.1451-0.33940.18160.2937-0.00191.0678-0.3482-0.291.40490.5921.6286-56.84386.1731116.7528
50.15320.1539-0.03480.21150.08960.4770.1954-0.4296-0.49340.4087-0.3011-0.20630.43490.42850.42641.33950.37910.63990.80270.7271.218-127.1923-22.065493.1461
60.1325-0.01270.02080.1764-0.11630.28090.2923-0.3412-0.22280.1371-0.0550.2532-0.02190.11540.41691.1220.0470.78530.95260.67881.1638-106.1131-17.358467.0582
70.15380.083-0.23950.1541-0.00770.4308-0.0759-0.4762-0.11180.27740.0517-0.21530.28170.34870.45151.32270.03810.56871.18350.54490.9278-150.07283.6108113.0662
80.03870.02740.00550.0390.02070.0391-0.05540.0735-0.15610.0436-0.06480.04350.00760.0201-0.01760.95170.13770.23350.83260.37860.4972-130.612216.910471.9573
90.02820.00970.00680.00410.00180.00190.0106-0.00780.00130.00240.0106-0.0043-0.0006-0.01760.00020.50530.06650.13170.3258-0.00190.5549-149.766634.749969.9858
100.0002-0.0008-0.0010.0054-0.00040.0039-0.03270.0659-0.0132-0.0244-0.0366-0.0008-0.0038-0.011801.01480.249-0.02520.8152-0.06290.646-113.951330.254293.4614
110.0014-00.00140.00110.00170.00260.01280.04560.017-0.06340.0028-0.0180.0068-0.038-01.03830.1894-0.33680.9217-0.03060.81-122.011525.631198.8068
120.0002-0.0019-0.00080.00350.00270.00150.014-0.02810.01020.0344-0.02860.0019-0.0206-0.0042-00.77920.3013-0.09131.10190.05890.8833-151.259412.802290.9153
130.0062-0.0014-0.00070.00030.00180.00360.02390.02020.01010.02590.04670.0087-0.01690.029501.14440.1688-0.16241.1616-0.22430.9612-138.324342.8575108.1553
140.0174-0.00580.01860.002-0.00640.01960.0144-0.0073-0.0102-0.01220.02140.0006-0.0113-0.0107-0.00010.78760.0677-0.02410.70490.13560.6429-126.766142.282998.0821
150.0166-0.00410.00970.0103-0.00760.0249-0.16450.1217-0.0011-0.0845-0.1465-0.34280.18770.3544-0.00044.2683-0.11040.29844.51090.03954.4632-100.065213.327482.1262
160.00990.0172-0.00550.0254-0.00740.01470.09590.0961-0.00330.016-0.01410.0119-0.07270.01560.00022.7974-0.2289-0.52742.4001-0.20072.3524-133.80178.047889.63
170.03480.0143-0.00010.01760.01530.02150.1144-0.0368-0.0056-0.10770.1108-0.0212-0.23080.05760.00032.9413-0.24690.61322.6840.41612.7615-135.53599.114793.4024
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)A7 - 229
2X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)B7 - 229
3X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)C1 - 17
4X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)C394 - 700
5X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)C833 - 997
6X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)D783 - 1069
7X-RAY DIFFRACTION2(chain 'C' and resseq 1006:1071) or (chain 'D' and resseq 621:778) or (chain 'D' and resseq 1103:1432) or (chain 'D' and resseq 1473:1499) or (chain 'E' )C1006 - 1071
8X-RAY DIFFRACTION2(chain 'C' and resseq 1006:1071) or (chain 'D' and resseq 621:778) or (chain 'D' and resseq 1103:1432) or (chain 'D' and resseq 1473:1499) or (chain 'E' )D621 - 778
9X-RAY DIFFRACTION2(chain 'C' and resseq 1006:1071) or (chain 'D' and resseq 621:778) or (chain 'D' and resseq 1103:1432) or (chain 'D' and resseq 1473:1499) or (chain 'E' )D1103 - 1432
10X-RAY DIFFRACTION2(chain 'C' and resseq 1006:1071) or (chain 'D' and resseq 621:778) or (chain 'D' and resseq 1103:1432) or (chain 'D' and resseq 1473:1499) or (chain 'E' )D1473 - 1499
11X-RAY DIFFRACTION2(chain 'C' and resseq 1006:1071) or (chain 'D' and resseq 621:778) or (chain 'D' and resseq 1103:1432) or (chain 'D' and resseq 1473:1499) or (chain 'E' )E2 - 94
12X-RAY DIFFRACTION3(chain 'C' and resseq 1083:1112) or (chain 'D' and resseq 2:131) or (chain 'D' and resseq 455:605) or (chain 'D' and resseq 1444:1468)C1083 - 1112
13X-RAY DIFFRACTION3(chain 'C' and resseq 1083:1112) or (chain 'D' and resseq 2:131) or (chain 'D' and resseq 455:605) or (chain 'D' and resseq 1444:1468)D2 - 131
14X-RAY DIFFRACTION3(chain 'C' and resseq 1083:1112) or (chain 'D' and resseq 2:131) or (chain 'D' and resseq 455:605) or (chain 'D' and resseq 1444:1468)D455 - 605
15X-RAY DIFFRACTION3(chain 'C' and resseq 1083:1112) or (chain 'D' and resseq 2:131) or (chain 'D' and resseq 455:605) or (chain 'D' and resseq 1444:1468)D1444 - 1468
16X-RAY DIFFRACTION4(chain 'D' and resseq 132:454)D132 - 454
17X-RAY DIFFRACTION5(chain 'C' and resseq 18:142) or (chain 'C' and resseq 332:393)C18 - 142
18X-RAY DIFFRACTION5(chain 'C' and resseq 18:142) or (chain 'C' and resseq 332:393)C332 - 393
19X-RAY DIFFRACTION6(chain 'C' and resseq 143:331)C143 - 331
20X-RAY DIFFRACTION7(chain 'C' and resseq 701:832)C701 - 832
21X-RAY DIFFRACTION8(chain 'D' and resseq 1070:1102)D0
22X-RAY DIFFRACTION9(chain 'D' and resseq 779:782)D779 - 782
23X-RAY DIFFRACTION10(chain 'D' and resseq 1433:1443)D0
24X-RAY DIFFRACTION11(chain 'D' and resseq 606:620)D606 - 620
25X-RAY DIFFRACTION12(chain 'C' and resseq 998:1005)C998 - 1005
26X-RAY DIFFRACTION13(chain 'C' and resseq 1072:1082)C0
27X-RAY DIFFRACTION14(chain 'D' and resseq 1469:1472)D0
28X-RAY DIFFRACTION15(chain 'N' ) or (chain 'T' and resseq 1:13)N0
29X-RAY DIFFRACTION16chain 'R'R21 - 29
30X-RAY DIFFRACTION17chain 'T' and resseq 14:22T14 - 22

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