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- PDB-2cw0: Crystal structure of Thermus thermophilus RNA polymerase holoenzy... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2cw0 | ||||||
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Title | Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution | ||||||
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![]() | TRANSFERASE / RNA polymerase holoenzyme / transcription / bent-bridge helix | ||||||
Function / homology | ![]() sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding ...sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding / zinc ion binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tuske, S. / Sarafianos, S.G. / Wang, X. / Hudson, B. / Sineva, E. / Mukhopadhyay, J. / Birktoft, J.J. / Leroy, O. / Ismail, S. / Clark Jr., A.D. ...Tuske, S. / Sarafianos, S.G. / Wang, X. / Hudson, B. / Sineva, E. / Mukhopadhyay, J. / Birktoft, J.J. / Leroy, O. / Ismail, S. / Clark Jr., A.D. / Dharia, C. / Napoli, A. / Laptenko, O. / Lee, J. / Borukhov, S. / Ebright, R.H. / Arnold, E. | ||||||
![]() | ![]() Title: Inhibition of bacterial RNA polymerase by streptolydigin: stabilization of a straight-bridge-helix active-center conformation Authors: Tuske, S. / Sarafianos, S.G. / Wang, X. / Hudson, B. / Sineva, E. / Mukhopadhyay, J. / Birktoft, J.J. / Leroy, O. / Ismail, S. / Clark Jr., A.D. / Dharia, C. / Napoli, A. / Laptenko, O. / ...Authors: Tuske, S. / Sarafianos, S.G. / Wang, X. / Hudson, B. / Sineva, E. / Mukhopadhyay, J. / Birktoft, J.J. / Leroy, O. / Ismail, S. / Clark Jr., A.D. / Dharia, C. / Napoli, A. / Laptenko, O. / Lee, J. / Borukhov, S. / Ebright, R.H. / Arnold, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.3 MB | Display | ![]() |
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PDB format | ![]() | 1 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1zyrC ![]() 1iw7S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
-DNA-directed RNA polymerase ... , 3 types, 8 molecules ABKLCMDN
#1: Protein | Mass: 35056.164 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Protein | Mass: 125436.539 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Protein | Mass: 170997.391 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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-RNA polymerase ... , 2 types, 4 molecules EOFP
#4: Protein | Mass: 11521.221 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #5: Protein | Mass: 48568.008 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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-Non-polymers , 2 types, 6 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/MG.gif)
#6: Chemical | ChemComp-ZN / #7: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.54 Å3/Da / Density % sol: 71 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.85 Details: 33mM magnesium formate over a reservoir of 33mM magnesium formate, 30mM sodium citrate, pH 5.85, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Oct 28, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 3.3→30 Å / Num. all: 115975 / Num. obs: 115975 / % possible obs: 98.5 % / Observed criterion σ(I): -1 / Rmerge(I) obs: 0.138 / Net I/σ(I): 8.16 |
Reflection shell | Resolution: 3.3→3.42 Å / % possible all: 93.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1IW7 Resolution: 3.3→30 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber Details: THIS IS A TWINNED STRUCTURE. THE TWINNING OPERATOR IS (H,K,L) -> (-H,-K,L) AND THE TWINNING FRACTION IS 0.5. THE CRYSTALS HAVE THE SYMMETRY OF SPACE GROUP P32 WITH PERFECT (50%) HEMIHEDRAL ...Details: THIS IS A TWINNED STRUCTURE. THE TWINNING OPERATOR IS (H,K,L) -> (-H,-K,L) AND THE TWINNING FRACTION IS 0.5. THE CRYSTALS HAVE THE SYMMETRY OF SPACE GROUP P32 WITH PERFECT (50%) HEMIHEDRAL TWINNING AND TWINNING OPERATOR (-H, -K, L), LEADING TO APPARENT HEXAGONAL (P6/M) INTENSITY SYMMETRY. PROCESSING AND SCALING WERE CARRIED OUT IN P65 FOLLOWED BY EXPANSION TO P32 FOR STRUCTURE SOLUTION AND REFINEMENT.
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.3→30 Å
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Refine LS restraints |
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