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- PDB-4gzy: Crystal structures of bacterial RNA Polymerase paused elongation ... -

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Basic information

Entry
Database: PDB / ID: 4gzy
TitleCrystal structures of bacterial RNA Polymerase paused elongation complexes
Components
  • (DNA-directed RNA polymerase subunit ...Polymerase) x 4
  • RNA transcriptTranscription (biology)
  • non-template DNA
  • template DNA
KeywordsTRANSCRIPTION/DNA/RNA / RNA Polymerase / transcription / paused transcription elongation complex / transcriptional pausing / DNA directed RNA transcription / TRANSCRIPTION-DNA-RNA complex
Function / homology
Function and homology information


DNA-directed RNA polymerase complex / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding / zinc ion binding / cytoplasm
Similarity search - Function
Rna Polymerase Beta Subunit; Chain: C,domain 2 / Rna Polymerase Beta Subunit; Chain: C,domain 2 - #10 / Rna Polymerase Beta Subunit; Chain: C, domain 4 / DNA-directed RNA polymerase, beta subunit, external 1 domain / : / DNA-directed RNA polymerase subunit beta', hybrid domain / Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; domain 3 / RNA polymerase Rpb2, domain 2 / Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; Domain 6 / DNA-directed RNA polymerase, subunit 2, domain 6 ...Rna Polymerase Beta Subunit; Chain: C,domain 2 / Rna Polymerase Beta Subunit; Chain: C,domain 2 - #10 / Rna Polymerase Beta Subunit; Chain: C, domain 4 / DNA-directed RNA polymerase, beta subunit, external 1 domain / : / DNA-directed RNA polymerase subunit beta', hybrid domain / Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; domain 3 / RNA polymerase Rpb2, domain 2 / Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; Domain 6 / DNA-directed RNA polymerase, subunit 2, domain 6 / RNA polymerase II, Rpb2 subunit, wall domain / RNA polymerase subunit, RPB6/omega / Eukaryotic RPB6 RNA polymerase subunit / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / DNA-directed RNA polymerase, insert domain / RNA polymerase, RBP11-like subunit / RNA Polymerase Alpha Subunit; Chain A, domain 2 / Gyrase A; domain 2 / DNA-directed RNA polymerase, omega subunit / DNA-directed RNA polymerase, subunit beta-prime, bacterial type / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase, alpha subunit / DNA-directed RNA polymerase beta subunit, bacterial-type / Beta Complex / RNA polymerase Rpb6 / RNA polymerase, subunit omega/Rpo6/RPB6 / RNA polymerase Rpb6 / RNA polymerase Rpb1, domain 3 superfamily / RNA polymerase Rpb1, clamp domain superfamily / RPB6/omega subunit-like superfamily / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 4 / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 5 / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / DNA-directed RNA polymerase, insert domain / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / RNA polymerase Rpb3/RpoA insert domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerases D / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase, RBP11-like subunit / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6 / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Roll / Alpha-Beta Complex / Beta Barrel / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
DNA / DNA (> 10) / RNA / RNA (> 10) / DNA-directed RNA polymerase subunit alpha / DNA-directed RNA polymerase subunit omega / DNA-directed RNA polymerase subunit beta' / DNA-directed RNA polymerase subunit beta
Similarity search - Component
Biological speciesThermus thermophilus (bacteria)
synthetic (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.5054 Å
AuthorsWeixlbaumer, A. / Leon, K. / Landick, R. / Darst, S.A.
CitationJournal: Cell(Cambridge,Mass.) / Year: 2013
Title: Structural basis of transcriptional pausing in bacteria.
Authors: Weixlbaumer, A. / Leon, K. / Landick, R. / Darst, S.A.
History
DepositionSep 6, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 13, 2013Provider: repository / Type: Initial release
Revision 1.1Nov 15, 2017Group: Refinement description / Category: software / Item: _software.name
Revision 1.2Jul 17, 2019Group: Data collection / Refinement description / Category: software
Item: _software.classification / _software.contact_author ..._software.classification / _software.contact_author / _software.contact_author_email / _software.language / _software.location / _software.name / _software.type / _software.version
Revision 1.3Sep 13, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_comp_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_label_atom_id / _pdbx_struct_conn_angle.ptnr2_label_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA-directed RNA polymerase subunit alpha
B: DNA-directed RNA polymerase subunit alpha
C: DNA-directed RNA polymerase subunit beta
D: DNA-directed RNA polymerase subunit beta'
E: DNA-directed RNA polymerase subunit omega
N: non-template DNA
R: RNA transcript
T: template DNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)399,53011
Polymers399,3748
Non-polymers1553
Water0
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area37640 Å2
ΔGint-200 kcal/mol
Surface area127450 Å2
MethodPISA
Unit cell
Length a, b, c (Å)207.210, 207.210, 203.223
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number152
Space group name H-MP3121

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Components

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DNA-directed RNA polymerase subunit ... , 4 types, 5 molecules ABCDE

#1: Protein DNA-directed RNA polymerase subunit alpha / Polymerase / RNAP subunit alpha / RNA polymerase subunit alpha / Transcriptase subunit alpha


Mass: 35056.164 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Gene: rpoA, TTHA1664 / Production host: Thermus thermophilus (bacteria) / Strain (production host): HB8 / References: UniProt: Q5SHR6, DNA-directed RNA polymerase
#2: Protein DNA-directed RNA polymerase subunit beta / Polymerase / RNAP subunit beta / RNA polymerase subunit beta / Transcriptase subunit beta


Mass: 125436.539 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Gene: rpoB, TTHA1813 / Production host: Thermus thermophilus (bacteria) / Strain (production host): HB8 / References: UniProt: Q8RQE9, DNA-directed RNA polymerase
#3: Protein DNA-directed RNA polymerase subunit beta' / Polymerase / RNAP subunit beta' / RNA polymerase subunit beta' / Transcriptase subunit beta'


Mass: 172378.812 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: genomic copy of rpoC replaced by a C-terminal 10His tagged version
Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Gene: rpoC, TTHA1812 / Production host: Thermus thermophilus (bacteria) / Strain (production host): HB8 / References: UniProt: Q8RQE8, DNA-directed RNA polymerase
#4: Protein DNA-directed RNA polymerase subunit omega / Polymerase / RNAP omega subunit / RNA polymerase omega subunit / Transcriptase subunit omega


Mass: 11533.316 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Gene: rpoZ, TTHA1561 / Production host: Thermus thermophilus (bacteria) / Strain (production host): HB8 / References: UniProt: Q8RQE7, DNA-directed RNA polymerase

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DNA chain , 2 types, 2 molecules NT

#5: DNA chain non-template DNA


Mass: 4000.624 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic (others)
#7: DNA chain template DNA


Mass: 6696.343 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic (others)

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RNA chain , 1 types, 1 molecules R

#6: RNA chain RNA transcript / Transcription (biology)


Mass: 9216.427 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic (others)

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Non-polymers , 2 types, 3 molecules

#8: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#9: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.15 Å3/Da / Density % sol: 61 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.2
Details: 3.5-6% PEG 8000, 0.1M MES pH 6.1-6.3, 0.3M LiCl, 10mM MgCl2, vapor diffusion, hanging drop, temperature 295K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: NSLS / Beamline: X29A / Wavelength: 1.075 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Jan 30, 2011
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.075 Å / Relative weight: 1
ReflectionResolution: 3.5→50 Å / Num. all: 63554 / Num. obs: 63554 / % possible obs: 100 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 9.9 % / Rmerge(I) obs: 0.149 / Net I/σ(I): 6.5
Reflection shell
Resolution (Å)Redundancy (%)Diffraction-ID% possible allRmerge(I) obs
3.5-3.565.5199.9
3.56-3.635.91100
3.63-3.6961100
3.69-3.776.21100
3.77-3.856.211000.975
3.85-3.946.311000.811
3.94-4.046.411000.652
4.04-4.156.611000.497
4.15-4.27711000.417
4.27-4.417.511000.332
4.41-4.578.311000.262
4.57-4.75911000.219
4.75-4.9712.711000.351
4.97-5.2315.111000.418
5.23-5.5515.111000.391
5.55-5.9815.111000.334
5.98-6.581511000.259
6.58-7.5314.811000.167
7.53-9.4814.311000.082
9.48-5013.711000.053

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Phasing

PhasingMethod: molecular replacement
Phasing MRMethod rotation: fast direct / Method translation: &STRIP%trans_method

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Processing

Software
NameVersionClassificationNB
PHENIX1.8_1069refinement
CNSrefinement
SCALEPACKdata scaling
PDB_EXTRACT3.11data extraction
HKL-2000data collection
DENZOdata reduction
CNSphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 2O5I

2o5i


Resolution: 3.5054→49.77 Å / Occupancy max: 1 / Occupancy min: 1 / SU ML: 0.6 / σ(F): 1.33 / Phase error: 36.54 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.3217 3184 5.03 %
Rwork0.2631 --
obs0.2661 63330 99.68 %
all-63554 -
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 117.3666 Å2
Refinement stepCycle: LAST / Resolution: 3.5054→49.77 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms23534 863 3 0 24400
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00524969
X-RAY DIFFRACTIONf_angle_d0.9533909
X-RAY DIFFRACTIONf_dihedral_angle_d15.9019678
X-RAY DIFFRACTIONf_chiral_restr0.0613835
X-RAY DIFFRACTIONf_plane_restr0.0054309
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.5054-3.55770.40741430.40012543X-RAY DIFFRACTION98
3.5577-3.61330.38381340.38892584X-RAY DIFFRACTION99
3.6133-3.67250.39041540.38442569X-RAY DIFFRACTION100
3.6725-3.73580.39491220.36912579X-RAY DIFFRACTION100
3.7358-3.80370.42191500.3782567X-RAY DIFFRACTION100
3.8037-3.87690.37761510.34082593X-RAY DIFFRACTION100
3.8769-3.9560.37861370.3222584X-RAY DIFFRACTION100
3.956-4.04190.37251570.30662592X-RAY DIFFRACTION100
4.0419-4.13590.32571080.28312626X-RAY DIFFRACTION100
4.1359-4.23930.35881050.28862636X-RAY DIFFRACTION100
4.2393-4.35390.36341340.26922577X-RAY DIFFRACTION100
4.3539-4.48190.35011340.25242618X-RAY DIFFRACTION100
4.4819-4.62650.30071190.23162616X-RAY DIFFRACTION100
4.6265-4.79170.26061270.23552612X-RAY DIFFRACTION100
4.7917-4.98340.29061400.23932623X-RAY DIFFRACTION100
4.9834-5.20990.29911510.25352601X-RAY DIFFRACTION100
5.2099-5.48430.31881610.2642611X-RAY DIFFRACTION100
5.4843-5.82740.34981300.26112639X-RAY DIFFRACTION100
5.8274-6.27660.34021310.25962639X-RAY DIFFRACTION100
6.2766-6.90670.32861750.25272616X-RAY DIFFRACTION100
6.9067-7.90270.29741390.22822645X-RAY DIFFRACTION100
7.9027-9.94350.25331380.20632695X-RAY DIFFRACTION100
9.9435-49.77520.29131440.22742781X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.81550.5006-0.43472.7713-0.38581.3297-0.37820.31010.1398-0.2137-0.00810.71850.0118-0.0457-0.16210.3046-0.1329-0.34870.61020.11830.38477.7245127.5712-66.1138
22.9978-1.181-0.49993.40960.72422.2969-0.25410.1442-0.05480.28870.0353-0.89520.17390.5395-0.05620.3839-0.0804-0.13490.75970.34860.5664116.4753110.8173-54.0651
33.30410.8038-0.34974.9208-1.55522.14230.00360.14910.69991.56070.0159-0.1847-0.0673-0.2389-0.11170.91070.195-0.27340.92990.32950.8432120.453983.4612-25.0403
41.4725-1.1789-0.70971.82110.94521.10130.0373-0.2698-0.69950.7272-0.02140.60280.43390.091600.91530.04350.03531.01680.17431.8386130.62129.8175-17.6408
51.07080.4010.68160.37930.47761.9763-0.1066-0.2899-0.90861.28870.47520.9771.7189-0.25980.69391.8734-0.26950.9880.87320.17461.507967.115672.4516-41.9194
61.51940.3348-0.49431.6696-0.5461.8702-0.0419-1.02640.38280.17090.33620.4515-0.06921.19150.00010.9572-0.01360.35971.1645-0.09761.111381.973158.1441-69.3778
70.7219-0.35040.87050.3445-0.53390.6266-0.166-0.929-0.49211.21240.2350.19080.71620.26660.02951.54590.06590.40081.54670.07270.874875.8445105.3813-22.023
80.4042-0.12830.46880.0244-0.2133-0.0723-0.2703-1.08640.1581-0.0034-0.04580.15820.0839-1.0443-0.01950.7876-0.17380.15111.20670.18440.547696.498696.935-63.5392
90.0463-0.0461-0.00210.12250.06030.05890.045-0.06820.02390.06480.15520.02590.0815-0.06340.25380.5454-0.80910.50790.14160.72750.0517101.092122.4003-65.3093
101.5203-1.7432-1.77842.82191.32312.6772-0.35670.2722-0.282-0.9137-0.33630.67910.0145-0.2227-0.96420.80860.16490.06590.76160.70461.0204117.905489.7403-43.0544
110.0047-0.01480.01120.0051-0.0066-0.0046-0.5616-0.11670.06130.22610.2783-0.2247-0.5746-0.762900.9406-0.1004-0.22820.66040.04290.7365109.424594.0003-37.7026
1200.00890.0011-0.00440.00440.00280.5491-0.1152-0.31190.43330.43370.3425-0.3134-0.058-00.71550.1312-0.01371.15360.25340.724882.5223111.947-43.9002
130.37880.21120.19580.42160.36790.35160.17680.02390.02010.1450.3303-0.04320.30570.09480.18721.60840.006-0.5620.61330.70590.2619115.7235116.4473-28.3916
140.3537-0.0177-0.05670.1393-0.15330.1699-0.0586-0.1079-0.03140.0064-0.0633-0.0305-0.0665-0.0065-0.0752-0.05180.8501-1.03190.65891.39880.3654120.8419106.8052-39.2103
150.17-0.26550.00940.44750.19830.0394-1.09451.2796-0.8021-0.6201-0.1571-0.6393.51840.2589-0.03465.34831.1756-0.05114.626-0.20085.8004110.682169.1805-53.7471
160.0906-0.0306-0.05-0.0334-0.05850.08010.9306-0.3138-0.17040.43610.1794-0.39530.67970.44440.00252.0188-0.3149-0.37533.18580.18461.968687.986394.7873-45.2904
170.2237-0.14410.05120.0679-0.00890.06460.08730.7713-1.22551.12451.1106-0.85730.61251.43850.00312.5148-0.45960.73552.8259-0.25522.875787.912696.8393-41.699
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)A7 - 229
2X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)B7 - 229
3X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)C1 - 17
4X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)C394 - 700
5X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)C833 - 997
6X-RAY DIFFRACTION1(chain 'A' ) or (chain 'B' ) or (chain 'C' and resseq 1:17) or (chain 'C' and resseq 394:700) or (chain 'C' and resseq 833:997) or (chain 'D' and resseq 783:1069)D783 - 1069
7X-RAY DIFFRACTION2(chain 'C' and resseq 1006:1071) or (chain 'D' and resseq 621:778) or (chain 'D' and resseq 1103:1432) or (chain 'D' and resseq 1473:1499) or (chain 'E' ) or (chain D and resseq 1602:1603)C1006 - 1071
8X-RAY DIFFRACTION2(chain 'C' and resseq 1006:1071) or (chain 'D' and resseq 621:778) or (chain 'D' and resseq 1103:1432) or (chain 'D' and resseq 1473:1499) or (chain 'E' ) or (chain D and resseq 1602:1603)D621 - 778
9X-RAY DIFFRACTION2(chain 'C' and resseq 1006:1071) or (chain 'D' and resseq 621:778) or (chain 'D' and resseq 1103:1432) or (chain 'D' and resseq 1473:1499) or (chain 'E' ) or (chain D and resseq 1602:1603)D1103 - 1432
10X-RAY DIFFRACTION2(chain 'C' and resseq 1006:1071) or (chain 'D' and resseq 621:778) or (chain 'D' and resseq 1103:1432) or (chain 'D' and resseq 1473:1499) or (chain 'E' ) or (chain D and resseq 1602:1603)D1473 - 1499
11X-RAY DIFFRACTION2(chain 'C' and resseq 1006:1071) or (chain 'D' and resseq 621:778) or (chain 'D' and resseq 1103:1432) or (chain 'D' and resseq 1473:1499) or (chain 'E' ) or (chain D and resseq 1602:1603)E2 - 94
12X-RAY DIFFRACTION2(chain 'C' and resseq 1006:1071) or (chain 'D' and resseq 621:778) or (chain 'D' and resseq 1103:1432) or (chain 'D' and resseq 1473:1499) or (chain 'E' ) or (chain D and resseq 1602:1603)D1602 - 1603
13X-RAY DIFFRACTION3(chain 'C' and resseq 1083:1112) or (chain 'D' and resseq 2:131) or (chain 'D' and resseq 455:605) or (chain 'D' and resseq 1444:1468) or (chain D and resseq 1601)C1083 - 1112
14X-RAY DIFFRACTION3(chain 'C' and resseq 1083:1112) or (chain 'D' and resseq 2:131) or (chain 'D' and resseq 455:605) or (chain 'D' and resseq 1444:1468) or (chain D and resseq 1601)D2 - 131
15X-RAY DIFFRACTION3(chain 'C' and resseq 1083:1112) or (chain 'D' and resseq 2:131) or (chain 'D' and resseq 455:605) or (chain 'D' and resseq 1444:1468) or (chain D and resseq 1601)D455 - 605
16X-RAY DIFFRACTION3(chain 'C' and resseq 1083:1112) or (chain 'D' and resseq 2:131) or (chain 'D' and resseq 455:605) or (chain 'D' and resseq 1444:1468) or (chain D and resseq 1601)D1444 - 1468
17X-RAY DIFFRACTION3(chain 'C' and resseq 1083:1112) or (chain 'D' and resseq 2:131) or (chain 'D' and resseq 455:605) or (chain 'D' and resseq 1444:1468) or (chain D and resseq 1601)D1601
18X-RAY DIFFRACTION4(chain 'D' and resseq 132:454)D132 - 454
19X-RAY DIFFRACTION5(chain 'C' and resseq 18:142) or (chain 'C' and resseq 332:393)C18 - 142
20X-RAY DIFFRACTION5(chain 'C' and resseq 18:142) or (chain 'C' and resseq 332:393)C332 - 393
21X-RAY DIFFRACTION6(chain 'C' and resseq 143:331)C143 - 331
22X-RAY DIFFRACTION7(chain 'C' and resseq 701:832)C701 - 832
23X-RAY DIFFRACTION8(chain 'D' and resseq 1070:1102)D0
24X-RAY DIFFRACTION9(chain 'D' and resseq 779:782)D779 - 782
25X-RAY DIFFRACTION10(chain 'D' and resseq 1433:1443)D0
26X-RAY DIFFRACTION11(chain 'D' and resseq 606:620)D606 - 620
27X-RAY DIFFRACTION12(chain 'C' and resseq 998:1005)C998 - 1005
28X-RAY DIFFRACTION13(chain 'C' and resseq 1072:1082)C0
29X-RAY DIFFRACTION14(chain 'D' and resseq 1469:1472)D0
30X-RAY DIFFRACTION15(chain 'N' ) or (chain 'T' and resseq 1:13)N0
31X-RAY DIFFRACTION16chain 'R'R21 - 29
32X-RAY DIFFRACTION17chain 'T' and resseq 14:22T14 - 22

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