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Yorodumi- PDB-4gpy: Crystal structure of the bacterial ribosomal decoding site in com... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4gpy | ||||||||||||||||||
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Title | Crystal structure of the bacterial ribosomal decoding site in complex with 6'-hydroxysisomicin | ||||||||||||||||||
Components | RNA (5'-R(*Keywords | RNA/ANTIBIOTIC / ribosome / aminoglycoside / RNA-ANTIBIOTIC complex / antiprotozoal activity / antibacterial activity | Function / homology | Chem-6HS / RNA / RNA (> 10) | Function and homology information Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | Authors | Kondo, J. / Koganei, M. / Maianti, J.P. / Ly, V.L. / Hanessian, S. | Citation | Journal: Chemmedchem / Year: 2013 | Title: Crystal structures of a bioactive 6'-hydroxy variant of sisomicin bound to the bacterial and protozoal ribosomal decoding sites Authors: Kondo, J. / Koganei, M. / Maianti, J.P. / Ly, V.L. / Hanessian, S. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4gpy.cif.gz | 36.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4gpy.ent.gz | 25.3 KB | Display | PDB format |
PDBx/mmJSON format | 4gpy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gp/4gpy ftp://data.pdbj.org/pub/pdb/validation_reports/gp/4gpy | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: RNA chain | Mass: 7370.424 Da / Num. of mol.: 2 / Source method: obtained synthetically #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 46.89 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Na Cacodylate, Spermine, NaCl, MPD , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NW12A / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Jun 16, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→47.8 Å / Num. obs: 3348 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.8→30 Å / Occupancy max: 1 / Occupancy min: 1
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Solvent computation | Bsol: 34.3561 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 103.49 Å2 / Biso mean: 43.0193 Å2 / Biso min: 9.55 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→30 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 10
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Xplor file |
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