aromatase / Defective CYP19A1 causes AEXS / positive regulation of estradiol secretion / androgen catabolic process / female genitalia development / syncytium formation / negative regulation of chronic inflammatory response / estrogen biosynthetic process / Estrogen biosynthesis / testosterone biosynthetic process ...aromatase / Defective CYP19A1 causes AEXS / positive regulation of estradiol secretion / androgen catabolic process / female genitalia development / syncytium formation / negative regulation of chronic inflammatory response / estrogen biosynthetic process / Estrogen biosynthesis / testosterone biosynthetic process / sterol metabolic process / prostate gland growth / mammary gland development / negative regulation of macrophage chemotaxis / steroid biosynthetic process / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen / aromatase activity / female gonad development / uterus development / steroid hydroxylase activity / Endogenous sterols / oxygen binding / response to estradiol / electron transfer activity / iron ion binding / endoplasmic reticulum membrane / heme binding / endoplasmic reticulum / membrane Similarity search - Function
Mass: 352.467 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C23H28O3
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 5.82 Å3/Da / Density % sol: 78.88 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion / pH: 7.4 Details: The enzyme-inhibitor complexes were prepared by the addition from 20mM stock solutions of compound 4 in PEG550 to 18 M (~1mg/ml) of aromatase, to give a final inhibitor concentration of 300 ...Details: The enzyme-inhibitor complexes were prepared by the addition from 20mM stock solutions of compound 4 in PEG550 to 18 M (~1mg/ml) of aromatase, to give a final inhibitor concentration of 300 uM. The mixture was incubated overnight at 4 C in 100mM potassium phosphate buffer pH 7.4 containing 20% glycerol, 20mM dithiothreitol, 0.5 M ASD and 1mM BDM. The complex was then concentrated to 25-30mg/ml using ultrafiltration. Protein was setup for crystallization using protein to cocktail ratios 2:1 and 3:1. The protein was mixed with reservoir cocktails of 24-30% polyethylene glycol 4000 in 50mM NaCl, 50mM Tris, pH 8.5 and vapor diffused in sealed 24-well sitting drop plates against corresponding reservoir solution., VAPOR DIFFUSION, temperature 277K
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