+Open data
-Basic information
Entry | Database: PDB / ID: 4fme | ||||||
---|---|---|---|---|---|---|---|
Title | EspG-Rab1-Arf6 complex | ||||||
Components |
| ||||||
Keywords | PROTEIN BINDING / alpha-beta fold / Rab1-GAP / Arf6 effector / Rab1 / Arf6 | ||||||
Function / homology | Function and homology information positive regulation of glycoprotein metabolic process / erythrocyte apoptotic process / growth hormone secretion / maintenance of postsynaptic density structure / regulation of dendritic spine development / melanosome transport / COPII-coated vesicle cargo loading / establishment of epithelial cell polarity / negative regulation of receptor-mediated endocytosis / protein localization to endosome ...positive regulation of glycoprotein metabolic process / erythrocyte apoptotic process / growth hormone secretion / maintenance of postsynaptic density structure / regulation of dendritic spine development / melanosome transport / COPII-coated vesicle cargo loading / establishment of epithelial cell polarity / negative regulation of receptor-mediated endocytosis / protein localization to endosome / negative regulation of dendrite development / ruffle assembly / negative regulation of protein localization to cell surface / regulation of Rac protein signal transduction / vesicle transport along microtubule / positive regulation of keratinocyte migration / positive regulation of focal adhesion disassembly / regulation of filopodium assembly / RAB geranylgeranylation / RAB GEFs exchange GTP for GDP on RABs / endocytic recycling / thioesterase binding / MET receptor recycling / filopodium membrane / Golgi Cisternae Pericentriolar Stack Reorganization / protein localization to cell surface / Flemming body / TBC/RABGAPs / COPII-mediated vesicle transport / COPI-dependent Golgi-to-ER retrograde traffic / virion assembly / transport vesicle membrane / cortical actin cytoskeleton organization / positive regulation of actin filament polymerization / hepatocyte apoptotic process / Golgi organization / cleavage furrow / autophagosome assembly / regulation of presynapse assembly / synaptic vesicle endocytosis / endocytic vesicle / endoplasmic reticulum to Golgi vesicle-mediated transport / endomembrane system / signaling adaptor activity / COPI-mediated anterograde transport / vesicle-mediated transport / ruffle / substrate adhesion-dependent cell spreading / cellular response to nerve growth factor stimulus / small monomeric GTPase / G protein activity / liver development / positive regulation of interleukin-8 production / protein localization to plasma membrane / positive regulation of protein secretion / positive regulation of protein localization to plasma membrane / intracellular protein transport / positive regulation of neuron projection development / autophagy / endocytosis / recycling endosome membrane / GDP binding / melanosome / cell migration / presynapse / Clathrin-mediated endocytosis / nervous system development / cell cortex / early endosome membrane / postsynapse / cell differentiation / early endosome / cell adhesion / endosome / defense response to bacterium / cadherin binding / cell cycle / cell division / cysteine-type endopeptidase activity / Golgi membrane / focal adhesion / GTPase activity / glutamatergic synapse / GTP binding / Golgi apparatus / endoplasmic reticulum / proteolysis / extracellular exosome / extracellular region / membrane / metal ion binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4.1 Å | ||||||
Authors | Shao, F. / Zhu, Y. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 2012 Title: Structurally Distinct Bacterial TBC-like GAPs Link Arf GTPase to Rab1 Inactivation to Counteract Host Defenses. Authors: Dong, N. / Zhu, Y. / Lu, Q. / Hu, L. / Zheng, Y. / Shao, F. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 4fme.cif.gz | 259.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb4fme.ent.gz | 211 KB | Display | PDB format |
PDBx/mmJSON format | 4fme.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fm/4fme ftp://data.pdbj.org/pub/pdb/validation_reports/fm/4fme | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
|
-Components
-Protein , 3 types, 6 molecules ADBECF
#1: Protein | Mass: 38903.648 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: O157 / Gene: espG / Plasmid: pGEX-6p-2 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q5WMC0, UniProt: Q7DB50*PLUS #2: Protein | Mass: 19477.084 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RAB1, RAB1A / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P62820 #3: Protein | Mass: 18458.146 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ARF6 / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P62330 |
---|
-Non-polymers , 5 types, 14 molecules
#4: Chemical | #5: Chemical | #6: Chemical | ChemComp-MG / #7: Chemical | #8: Water | ChemComp-HOH / | |
---|
-Details
Sequence details | RESIDUES WERE CONFIRMED BY GENE SEQUENCING |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 3.88 Å3/Da / Density % sol: 68.26 % |
---|---|
Crystal grow | Temperature: 298 K / pH: 6.2 Details: 8% PEG8000, 0.1 M NaKPO4 (pH 6.2), and 0.2 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97923 |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Apr 12, 2011 |
Radiation | Monochromator: SI 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97923 Å / Relative weight: 1 |
Reflection | Resolution: 4.1→50 Å / Num. obs: 18452 / % possible obs: 99.8 % / Observed criterion σ(I): 1 / Redundancy: 5.7 % / Rmerge(I) obs: 0.129 / Net I/σ(I): 5.8 |
Reflection shell | Resolution: 4.1→4.25 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.663 / % possible all: 99.6 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 4.1→50 Å / Occupancy max: 1 / Occupancy min: 1 / σ(F): 0 Stereochemistry target values: MAXIMUM LIKELIHOOD TARGET USING AMPLITUDES
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Bsol: 81.83 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 150.5 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.1→50 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 4.1→4.25 Å / Total num. of bins used: 10
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
|