- PDB-4fbk: Crystal structure of a covalently fused Nbs1-Mre11 complex with o... -
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Basic information
Entry
Database: PDB / ID: 4fbk
Title
Crystal structure of a covalently fused Nbs1-Mre11 complex with one manganese ion per active site
Components
DNA repair and telomere maintenance protein nbs1,DNA repair protein rad32 CHIMERIC PROTEIN
Keywords
HYDROLASE / PROTEIN BINDING / DNA double-strand break repair / Nuclease / Schizosaccharomyces pombe
Function / homology
Function and homology information
Cytosolic sensors of pathogen-associated DNA / DNA Damage/Telomere Stress Induced Senescence / HDR through MMEJ (alt-NHEJ) / Sensing of DNA Double Strand Breaks / Processing of DNA double-strand break ends / mitochondrial double-strand break repair via homologous recombination / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / Mre11 complex / chromosome, telomeric repeat region / meiotic DNA double-strand break formation ...Cytosolic sensors of pathogen-associated DNA / DNA Damage/Telomere Stress Induced Senescence / HDR through MMEJ (alt-NHEJ) / Sensing of DNA Double Strand Breaks / Processing of DNA double-strand break ends / mitochondrial double-strand break repair via homologous recombination / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / Mre11 complex / chromosome, telomeric repeat region / meiotic DNA double-strand break formation / phosphorylation-dependent protein binding / DNA end binding / Y-form DNA binding / chromatin-protein adaptor activity / double-strand break repair involved in meiotic recombination / DNA double-strand break processing / nuclease activity / single-stranded DNA endodeoxyribonuclease activity / double-stranded DNA 3'-5' DNA exonuclease activity / telomere maintenance via recombination / single-stranded DNA 3'-5' DNA exonuclease activity / reciprocal meiotic recombination / mitotic intra-S DNA damage checkpoint signaling / DNA duplex unwinding / protein localization to chromosome, telomeric region / mitotic G2 DNA damage checkpoint signaling / telomere maintenance / double-strand break repair via homologous recombination / double-strand break repair via nonhomologous end joining / double-strand break repair / site of double-strand break / manganese ion binding / damaged DNA binding / molecular adaptor activity / Hydrolases; Acting on ester bonds / DNA repair / nucleus Similarity search - Function
A: DNA repair and telomere maintenance protein nbs1,DNA repair protein rad32 CHIMERIC PROTEIN B: DNA repair and telomere maintenance protein nbs1,DNA repair protein rad32 CHIMERIC PROTEIN hetero molecules
Mass: 18.015 Da / Num. of mol.: 210 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.48 Å3/Da / Density % sol: 50.4 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 100 MM Tri Na Citrate pH 5.0, 200 mM ammonium sulfate, 13% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
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