- PDB-4ec6: Ntf2-like, potential transfer protein TraM from Gram-positive con... -
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Open data
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Basic information
Entry
Database: PDB / ID: 4ec6
Title
Ntf2-like, potential transfer protein TraM from Gram-positive conjugative plasmid pIP501
Components
Putative uncharacterized protein
Keywords
UNKNOWN FUNCTION / Gram-positive / conjugation / NTF2-like
Function / homology
Nuclear Transport Factor 2; Chain: A, - #540 / Nuclear Transport Factor 2; Chain: A, / membrane => GO:0016020 / Roll / Alpha Beta / Uncharacterized protein
Function and homology information
Biological species
Enterococcus faecalis (bacteria)
Method
X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.5 Å
A: Putative uncharacterized protein B: Putative uncharacterized protein C: Putative uncharacterized protein D: Putative uncharacterized protein E: Putative uncharacterized protein F: Putative uncharacterized protein
Mass: 18.015 Da / Num. of mol.: 399 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 1.73 Å3/Da / Density % sol: 28.81 %
Crystal grow
Temperature: 298 K / Method: microbatch under oil / pH: 7.33 Details: 1 mg/ml protein, 16.5% PEG 3350 (final concentration in drop v/v), 0.1 M Hepes, pH 7.33, microbatch under oil, temperature 298K
Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: May 29, 2011 Details: toroidal mirror (M2) to vertically and horizontally focus the beam at the sample position (with 2:1 horizontal demagnification)
Radiation
Monochromator: BARTELS MONOCROMATOR / Protocol: SAD / Scattering type: x-ray
Method to determine structure: SAD / Resolution: 2.5→46.89 Å / Cor.coef. Fo:Fc: 0.913 / Cor.coef. Fo:Fc free: 0.859 / Occupancy max: 1 / Occupancy min: 1 / SU B: 9.8 / SU ML: 0.22 / SU R Cruickshank DPI: 1.1139 / Cross valid method: THROUGHOUT / ESU R: 1.114 / ESU R Free: 0.325 Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.26109
1295
5 %
RANDOM
Rwork
0.20921
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obs
0.2118
24589
97.87 %
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all
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52900
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
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