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- PDB-4e7h: PFV intasome prior to 3'-processing, Apo form (UI-Apo) -

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Basic information

Entry
Database: PDB / ID: 4e7h
TitlePFV intasome prior to 3'-processing, Apo form (UI-Apo)
Components
  • DNA (5'-D(*AP*TP*TP*GP*TP*CP*AP*TP*GP*GP*AP*AP*TP*TP*TP*CP*GP*CP*A)-3')
  • DNA (5'-D(*TP*GP*CP*GP*AP*AP*AP*TP*TP*CP*CP*AP*TP*GP*AP*CP*AP*AP*T)-3')
  • Pro-Pol polyprotein
KeywordsRECOMBINATION/DNA / protein-DNA complex / tetramer / HHCC motif / endonuclease / metal-binding / multifunctional enzyme / nuclease / nucleotidyltransferase / nucleus / transferase / virion / DNA-binding / zinc-binding / viral protein / recombination / viral protein-DNA complex / RECOMBINATION-DNA complex
Function / homology
Function and homology information


Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / ribonuclease H / DNA integration / viral genome integration into host DNA / viral penetration into host nucleus / establishment of integrated proviral latency / RNA-directed DNA polymerase / RNA-directed DNA polymerase activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / RNA-DNA hybrid ribonuclease activity ...Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / ribonuclease H / DNA integration / viral genome integration into host DNA / viral penetration into host nucleus / establishment of integrated proviral latency / RNA-directed DNA polymerase / RNA-directed DNA polymerase activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / RNA-DNA hybrid ribonuclease activity / DNA recombination / Hydrolases; Acting on ester bonds / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / aspartic-type endopeptidase activity / host cell cytoplasm / viral entry into host cell / host cell nucleus / RNA binding / identical protein binding / metal ion binding
Similarity search - Function
Arc Repressor Mutant, subunit A - #110 / Endonuclease III; domain 1 - #70 / Retroviral integrase C-terminal SH3 domain / Spumavirus aspartic protease (A9) / Foamy virus protease (FV PR) domain profile. / Spumavirus aspartic protease A9 / Retroviral integrase, C-terminal SH3 domain / Integrase zinc-binding domain / Integrase zinc binding domain / Arc Repressor Mutant, subunit A ...Arc Repressor Mutant, subunit A - #110 / Endonuclease III; domain 1 - #70 / Retroviral integrase C-terminal SH3 domain / Spumavirus aspartic protease (A9) / Foamy virus protease (FV PR) domain profile. / Spumavirus aspartic protease A9 / Retroviral integrase, C-terminal SH3 domain / Integrase zinc-binding domain / Integrase zinc binding domain / Arc Repressor Mutant, subunit A / RNase H-like domain found in reverse transcriptase / Reverse transcriptase/retrotransposon-derived protein, RNase H-like domain / Endonuclease III; domain 1 / SH3 type barrels. - #140 / Ribonuclease H-like superfamily/Ribonuclease H / Helix non-globular / RNase H / Integrase core domain / Integrase catalytic domain profile. / Integrase, catalytic core / Ribonuclease H domain / Reverse transcriptase domain / Reverse transcriptase (RNA-dependent DNA polymerase) / Reverse transcriptase (RT) catalytic domain profile. / RNase H type-1 domain profile. / Special / SH3 type barrels. / Nucleotidyltransferase; domain 5 / Ribonuclease H superfamily / Ribonuclease H-like superfamily / Aspartic peptidase domain superfamily / Reverse transcriptase/Diguanylate cyclase domain / Roll / DNA/RNA polymerase superfamily / 2-Layer Sandwich / Orthogonal Bundle / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
HEXANE-1,6-DIOL / DNA (> 10) / DNA / Pro-Pol polyprotein
Similarity search - Component
Biological speciesHuman spumaretrovirus
Synthetic DNA (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5701 Å
AuthorsHare, S. / Cherepanov, P.
CitationJournal: Embo J. / Year: 2012
Title: 3'-Processing and strand transfer catalysed by retroviral integrase in crystallo.
Authors: Hare, S. / Maertens, G.N. / Cherepanov, P.
History
DepositionMar 17, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 23, 2012Provider: repository / Type: Initial release
Revision 1.1Jul 25, 2012Group: Database references

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Pro-Pol polyprotein
B: Pro-Pol polyprotein
C: DNA (5'-D(*AP*TP*TP*GP*TP*CP*AP*TP*GP*GP*AP*AP*TP*TP*TP*CP*GP*CP*A)-3')
D: DNA (5'-D(*TP*GP*CP*GP*AP*AP*AP*TP*TP*CP*CP*AP*TP*GP*AP*CP*AP*AP*T)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)102,05420
Polymers100,5614
Non-polymers1,49316
Water4,179232
1
A: Pro-Pol polyprotein
B: Pro-Pol polyprotein
C: DNA (5'-D(*AP*TP*TP*GP*TP*CP*AP*TP*GP*GP*AP*AP*TP*TP*TP*CP*GP*CP*A)-3')
D: DNA (5'-D(*TP*GP*CP*GP*AP*AP*AP*TP*TP*CP*CP*AP*TP*GP*AP*CP*AP*AP*T)-3')
hetero molecules

A: Pro-Pol polyprotein
B: Pro-Pol polyprotein
C: DNA (5'-D(*AP*TP*TP*GP*TP*CP*AP*TP*GP*GP*AP*AP*TP*TP*TP*CP*GP*CP*A)-3')
D: DNA (5'-D(*TP*GP*CP*GP*AP*AP*AP*TP*TP*CP*CP*AP*TP*GP*AP*CP*AP*AP*T)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)204,10740
Polymers201,1228
Non-polymers2,98532
Water1448
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation8_554-y,-x,-z-1/21
Buried area33110 Å2
ΔGint-319 kcal/mol
Surface area55330 Å2
MethodPISA
Unit cell
γ
α
β
Length a, b, c (Å)159.470, 159.470, 123.750
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number92
Space group name H-MP41212

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Pro-Pol polyprotein / Pr125Pol / Protease/Reverse transcriptase/ribonuclease H / p87Pro-RT-RNaseH / Protease/Reverse ...Pr125Pol / Protease/Reverse transcriptase/ribonuclease H / p87Pro-RT-RNaseH / Protease/Reverse transcriptase / p65Pro-RT / Ribonuclease H / RNase H / Integrase / IN / p42In


Mass: 44456.695 Da / Num. of mol.: 2 / Fragment: UNP Residues 752-1143
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human spumaretrovirus / Strain: HSRV2 / Gene: pol / Plasmid: pSSH6P-PFV-INFL / Production host: Escherichia coli (E. coli) / Strain (production host): PC2
References: UniProt: P14350, RNA-directed DNA polymerase, DNA-directed DNA polymerase, ribonuclease H, Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases

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DNA chain , 2 types, 2 molecules CD

#2: DNA chain DNA (5'-D(*AP*TP*TP*GP*TP*CP*AP*TP*GP*GP*AP*AP*TP*TP*TP*CP*GP*CP*A)-3')


Mass: 5834.794 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: viral DNA / Source: (synth.) Synthetic DNA (others)
#3: DNA chain DNA (5'-D(*TP*GP*CP*GP*AP*AP*AP*TP*TP*CP*CP*AP*TP*GP*AP*CP*AP*AP*T)-3')


Mass: 5812.799 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Synthetic DNA (others)

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Non-polymers , 5 types, 248 molecules

#4: Chemical
ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: SO4
#5: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 9 / Source method: obtained synthetically / Formula: C3H8O3
#6: Chemical ChemComp-HEZ / HEXANE-1,6-DIOL / 1,6-Hexanediol


Mass: 118.174 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H14O2
#7: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn
#8: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 232 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.91 Å3/Da / Density % sol: 68.56 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.5
Details: 1.35 M AMMONIUM SULFATE, 25% (V/V), GLYCEROL, 4.8% (V/V) 1,6-HEXANEDIOL, 50 MM MES-NAOH, 2MM EDTA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 1.0389 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 17, 2011
RadiationMonochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.0389 Å / Relative weight: 1
ReflectionResolution: 2.57→40 Å / Num. obs: 50886 / % possible obs: 99.5 % / Observed criterion σ(I): -3 / Redundancy: 5.1 % / Rmerge(I) obs: 0.085 / Net I/σ(I): 12.9
Reflection shellResolution: 2.57→2.71 Å / Redundancy: 5.2 % / Rmerge(I) obs: 0.849 / Mean I/σ(I) obs: 2 / % possible all: 100

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Processing

Software
NameVersionClassification
PHENIX(phenix.refine: 1.7.3_928)refinement
MOSFLMdata reduction
SCALAdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 3OY9
Resolution: 2.5701→36.916 Å / SU ML: 0.45 / σ(F): 1.35 / Phase error: 20.92 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2189 2562 5.04 %
Rwork0.1863 --
obs0.188 50851 99.24 %
Solvent computationShrinkage radii: 0.98 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 59.704 Å2 / ksol: 0.34 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1-1.9846 Å20 Å2-0 Å2
2--1.9846 Å2-0 Å2
3----3.9693 Å2
Refinement stepCycle: LAST / Resolution: 2.5701→36.916 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4371 773 88 232 5464
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0095454
X-RAY DIFFRACTIONf_angle_d1.3647587
X-RAY DIFFRACTIONf_dihedral_angle_d19.3332067
X-RAY DIFFRACTIONf_chiral_restr0.075853
X-RAY DIFFRACTIONf_plane_restr0.006822
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.5701-2.61950.39891350.35432667X-RAY DIFFRACTION100
2.6195-2.67290.35921440.3272643X-RAY DIFFRACTION100
2.6729-2.7310.35391570.30882645X-RAY DIFFRACTION100
2.731-2.79450.35471390.29562679X-RAY DIFFRACTION100
2.7945-2.86440.31531490.2622638X-RAY DIFFRACTION100
2.8644-2.94180.26051470.2372678X-RAY DIFFRACTION100
2.9418-3.02830.28141200.20512678X-RAY DIFFRACTION100
3.0283-3.1260.24041370.19732687X-RAY DIFFRACTION100
3.126-3.23770.23821340.18092664X-RAY DIFFRACTION100
3.2377-3.36720.21171360.16962704X-RAY DIFFRACTION100
3.3672-3.52040.20631520.17142658X-RAY DIFFRACTION100
3.5204-3.70580.18351350.17312685X-RAY DIFFRACTION100
3.7058-3.93780.20241490.17682688X-RAY DIFFRACTION99
3.9378-4.24140.18591530.1642684X-RAY DIFFRACTION100
4.2414-4.66750.19191600.14372697X-RAY DIFFRACTION99
4.6675-5.34110.18011520.15482711X-RAY DIFFRACTION99
5.3411-6.72260.21761280.18232764X-RAY DIFFRACTION99
6.7226-36.91990.21321350.18972719X-RAY DIFFRACTION93

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