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- PDB-4dr7: Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal... -

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Basic information

Entry
Database: PDB / ID: 4dr7
TitleCrystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with codon, crystallographically disordered near-cognate transfer RNA anticodon stem-loop mismatched at the second codon position, and streptomycin bound
Components
  • (30S ribosomal protein ...) x 20
  • 16S rRNA
  • 5'-R(*GP*CP*CP*UP*GP*GP*AP*AP*AP*GP*(PSU))-3'
  • 5'-R(*UP*UP*UP*U)-3'
  • 5'-R(P*UP*GP*GP*AP*AP*AP*GP*(PSU))-3'
  • 5'-R(P*UP*UP*U)-3'
KeywordsRIBOSOME/ANTIBIOTIC / decoding / Streptomycin / RNA structure / antibiotic resistance / RIBOSOME-ANTIBIOTIC complex
Function / homology
Function and homology information


small ribosomal subunit / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / zinc ion binding / metal ion binding / cytoplasm
Similarity search - Function
Ribosomal protein S4/S9, N-terminal domain / Ribosomal Protein S4 Delta 41; Chain A, domain 1 / S16 Ribosomal Protein; Chain: A; / Ribosomal protein S16 / Ribosomal protein S18 / 30s Ribosomal Protein S18 / 30s ribosomal protein s13; domain 2 / Ribosomal protein S13/S18, C-terminal domain / Ribosomal protein S20 / 30s Ribosomal Protein S19; Chain A ...Ribosomal protein S4/S9, N-terminal domain / Ribosomal Protein S4 Delta 41; Chain A, domain 1 / S16 Ribosomal Protein; Chain: A; / Ribosomal protein S16 / Ribosomal protein S18 / 30s Ribosomal Protein S18 / 30s ribosomal protein s13; domain 2 / Ribosomal protein S13/S18, C-terminal domain / Ribosomal protein S20 / 30s Ribosomal Protein S19; Chain A / Ribosomal protein S19/S15 / Ribosomal protein S3, C-terminal domain / Ribosomal Protein S14/S29 / 30s Ribosomal Protein S14; Chain N / Ribosomal Protein S8; Chain: A, domain 1 - #30 / RNA-binding S4 domain / Ribosomal Protein S7 / Ribosomal protein S7/S5 / Ribosomal protein S6/Translation elongation factor EF1B / Ribosomal protein S3 C-terminal domain / Helix hairpin bin / Dna Ligase; domain 1 - #10 / Ribosomal protein S11/S14 / Ribosomal protein S10 / Structural Genomics Hypothetical 15.5 Kd Protein In mrcA-pckA Intergenic Region; Chain A / S15/NS1, RNA-binding / K homology (KH) domain / Double Stranded RNA Binding Domain - #20 / Helicase, Ruva Protein; domain 3 - #50 / Glucose-6-phosphate isomerase like protein; domain 1 / Ribosomal Protein S8; Chain: A, domain 1 / Ribosomal Protein S5; domain 2 - #10 / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein / GMP Synthetase; Chain A, domain 3 / Ribosomal Protein S5; domain 2 / Double Stranded RNA Binding Domain / Ribosomal protein S14, type Z / Helicase, Ruva Protein; domain 3 / Nucleic acid-binding proteins / Dna Ligase; domain 1 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / Ribosomal protein S14/S29 / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / K Homology domain / K homology RNA-binding domain / Ribosomal protein S3, conserved site / Ribosomal protein S14, conserved site / Ribosomal protein S10, conserved site / : / K Homology domain, type 2 / Ribosomal protein S3, C-terminal / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S15/S19, conserved site / KH domain / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S10 / Ribosomal protein S3, C-terminal domain / Few Secondary Structures / Irregular / Ribosomal protein S3 signature. / Ribosomal protein S10 signature. / Ribosomal protein S14 signature. / Ribosomal protein S7, conserved site / Ribosomal protein S17, conserved site / K homology domain superfamily, prokaryotic type / Ribosomal protein S19 / Ribosomal protein S2 signature 1. / Ribosomal protein S13, conserved site
Similarity search - Domain/homology
STREPTOMYCIN / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein uS8 ...STREPTOMYCIN / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS19 / 30S ribosomal protein S17 / 30S ribosomal protein S14 type Z / 30S ribosomal protein S8 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein bTHX / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS18
Similarity search - Component
Biological speciesThermus thermophilus (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 3.75 Å
AuthorsDemirci, H. / Murphy IV, F. / Murphy, E. / Gregory, S.T. / Dahlberg, A.E. / Jogl, G.
CitationJournal: Nat Commun / Year: 2013
Title: A structural basis for streptomycin-induced misreading of the genetic code.
Authors: Demirci, H. / Murphy, F. / Murphy, E. / Gregory, S.T. / Dahlberg, A.E. / Jogl, G.
History
DepositionFeb 16, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 14, 2012Provider: repository / Type: Initial release
Revision 1.1Jan 23, 2013Group: Database references
Revision 1.2Jan 30, 2013Group: Database references

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 16S rRNA
B: 30S ribosomal protein S2
C: 30S ribosomal protein S3
D: 30S ribosomal protein S4
E: 30S ribosomal protein S5
F: 30S ribosomal protein S6
G: 30S ribosomal protein S7
H: 30S ribosomal protein S8
I: 30S ribosomal protein S9
J: 30S ribosomal protein S10
K: 30S ribosomal protein S11
L: 30S ribosomal protein S12
M: 30S ribosomal protein S13
N: 30S ribosomal protein S14
O: 30S ribosomal protein S15
P: 30S ribosomal protein S16
Q: 30S ribosomal protein S17
R: 30S ribosomal protein S18
S: 30S ribosomal protein S19
T: 30S ribosomal protein S20
U: 30S ribosomal protein THX
V: 5'-R(*UP*UP*UP*U)-3'
W: 5'-R(*GP*CP*CP*UP*GP*GP*AP*AP*AP*GP*(PSU))-3'
a: 5'-R(P*UP*GP*GP*AP*AP*AP*GP*(PSU))-3'
b: 5'-R(P*UP*UP*U)-3'
hetero molecules


Theoretical massNumber of molelcules
Total (without water)801,199372
Polymers792,12625
Non-polymers9,073347
Water16,069892
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)402.490, 402.490, 174.790
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number92
Space group name H-MP41212

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Components

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RNA chain , 5 types, 5 molecules AVWab

#1: RNA chain 16S rRNA /


Mass: 494170.781 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: GenBank: M26923.1
#22: RNA chain 5'-R(*UP*UP*UP*U)-3'


Mass: 1179.706 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: mRNA A site / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8
#23: RNA chain 5'-R(*GP*CP*CP*UP*GP*GP*AP*AP*AP*GP*(PSU))-3'


Mass: 3546.178 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: ASL (Serine) A site / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8
#24: RNA chain 5'-R(P*UP*GP*GP*AP*AP*AP*GP*(PSU))-3'


Mass: 2590.609 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: ASL (Serine) Shine Dalgarno mini helix / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8
#25: RNA chain 5'-R(P*UP*UP*U)-3'


Mass: 873.540 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: mRNA P site / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8

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30S ribosomal protein ... , 20 types, 20 molecules BCDEFGHIJKLMNOPQRSTU

#2: Protein 30S ribosomal protein S2 /


Mass: 29317.703 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80371
#3: Protein 30S ribosomal protein S3 /


Mass: 26751.076 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80372
#4: Protein 30S ribosomal protein S4 /


Mass: 24373.447 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80373
#5: Protein 30S ribosomal protein S5 /


Mass: 17583.416 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHQ5
#6: Protein 30S ribosomal protein S6 /


Mass: 11988.753 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SLP8
#7: Protein 30S ribosomal protein S7 /


Mass: 18050.973 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P17291
#8: Protein 30S ribosomal protein S8 /


Mass: 15868.570 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHQ2, UniProt: P0DOY9*PLUS
#9: Protein 30S ribosomal protein S9 /


Mass: 14410.614 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80374
#10: Protein 30S ribosomal protein S10 /


Mass: 11954.968 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHN7
#11: Protein 30S ribosomal protein S11 /


Mass: 13737.868 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80376
#12: Protein 30S ribosomal protein S12 /


Mass: 14966.846 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: F6DEQ7, UniProt: Q5SHN3*PLUS
#13: Protein 30S ribosomal protein S13 /


Mass: 14338.861 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80377
#14: Protein 30S ribosomal protein S14 /


Mass: 7158.725 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHQ1, UniProt: P0DOY6*PLUS
#15: Protein 30S ribosomal protein S15 /


Mass: 10578.407 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SJ76
#16: Protein 30S ribosomal protein S16 /


Mass: 10409.983 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SJH3
#17: Protein 30S ribosomal protein S17 /


Mass: 12324.670 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHP7, UniProt: P0DOY7*PLUS
#18: Protein 30S ribosomal protein S18 /


Mass: 10258.299 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SLQ0
#19: Protein 30S ribosomal protein S19 /


Mass: 10605.464 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHP2
#20: Protein 30S ribosomal protein S20 /


Mass: 11736.143 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80380
#21: Protein/peptide 30S ribosomal protein THX / Ribosome


Mass: 3350.030 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SIH3

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Non-polymers , 4 types, 1239 molecules

#26: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 344 / Source method: obtained synthetically / Formula: Mg
#27: Chemical ChemComp-SRY / STREPTOMYCIN / STREPTOMYCIN A / Streptomycin


Mass: 581.574 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C21H39N7O12 / Comment: medication, antibiotic*YM
#28: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#29: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 892 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.47 Å3/Da / Density % sol: 72.47 %
Crystal growTemperature: 277 K / Method: hanging drop / pH: 6.5 / Details: MPD, pH 6.5, HANGING DROP, temperature 277K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1.1 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 28, 2011
RadiationMonochromator: double crystal Si(111) monochromator with cryogenically-cooled first crystal and sagittally-bent second crystal horizontally-focusing at 3.3:1 demagnification
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.1 Å / Relative weight: 1
ReflectionNumber: 1645967 / Rmerge(I) obs: 0.136 / D res high: 3.75 Å / Num. obs: 146049 / % possible obs: 99.9
Diffraction reflection shell
Highest resolution (Å)Lowest resolution (Å)Num. obs% possible obs (%)IDRmerge(I) obs
16.775017509510.03
11.8616.77314210010.036
9.6811.86400610010.04
8.399.68468610010.05
7.58.39528410010.058
6.857.5580910010.068
6.346.85628810010.091
5.936.34672910010.111
5.595.93715810010.122
5.35.59753110010.147
5.065.3792510010.172
4.845.06826910010.208
4.654.84863710010.26
4.484.65891210010.307
4.334.48927310010.411
4.194.33955910010.532
4.074.19983710010.686
3.954.071013610010.9
3.853.951041499.811.212
3.753.851070410011.446
ReflectionResolution: 3.75→50 Å / Num. all: 146049 / Num. obs: 146049 / % possible obs: 99.9 % / Observed criterion σ(F): -3 / Observed criterion σ(I): -3 / Biso Wilson estimate: 121.622 Å2 / Rmerge(I) obs: 0.136 / Net I/σ(I): 15.52
Reflection shell
Resolution (Å)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. unique obsDiffraction-ID% possible all
3.75-3.850.0141.99122316107041100
3.85-3.950.0142.3711285910414199.8
3.95-4.070.0143.25118439101361100
4.07-4.190.0144.2311364298371100
4.19-4.330.0145.3810641795591100
4.33-4.480.0146.8310691292731100
4.48-4.650.0148.9210367089121100
4.65-4.840.01410.359790086371100
4.84-5.060.01412.529238382691100
5.06-5.30.014159194579251100
5.3-5.590.01416.978376275311100
5.59-5.930.01419.948248071581100
5.93-6.340.01421.597734167291100
6.34-6.850.01425.046903462881100
6.85-7.50.01432.176639258091100
7.5-8.390.01436.715701852841100
8.39-9.680.01443.215234246861100
9.68-11.860.01450.724220740061100
11.86-16.770.01456.373248131421100
16.77-500.01459.28164271750195

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Processing

Software
NameVersionClassificationNB
XSCALEdata scaling
PHENIXdev_978refinement
PDB_EXTRACT3.1data extraction
HKL-2000data collection
XDSdata reduction
PHENIXdev_978phasing
RefinementMethod to determine structure: FOURIER SYNTHESIS / Resolution: 3.75→48.909 Å / Occupancy max: 1 / Occupancy min: 0.19 / FOM work R set: 0.8842 / SU ML: 0.3 / σ(F): 1.99 / Phase error: 18.48 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2008 7300 5 %FROM PDB ENTRY 2VQE
Rwork0.1481 ---
obs0.1507 146005 99.94 %-
all-146005 --
Solvent computationShrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 68.73 Å2 / ksol: 0.25 e/Å3
Displacement parametersBiso max: 550 Å2 / Biso mean: 146.2181 Å2 / Biso min: 33.79 Å2
Baniso -1Baniso -2Baniso -3
1--3.9266 Å20 Å20 Å2
2---3.9266 Å20 Å2
3---13.4395 Å2
Refinement stepCycle: LAST / Resolution: 3.75→48.909 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms19127 33073 386 892 53478
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01457593
X-RAY DIFFRACTIONf_angle_d1.62786052
X-RAY DIFFRACTIONf_chiral_restr0.08610574
X-RAY DIFFRACTIONf_plane_restr0.0094933
X-RAY DIFFRACTIONf_dihedral_angle_d20.41126471
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30 / % reflection obs: 100 %

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all
3.75-3.79260.29652370.236545844821
3.7926-3.83720.29262560.23545434799
3.8372-3.88390.27232810.23145524833
3.8839-3.93310.28231940.226545704764
3.9331-3.98480.22812510.197245804831
3.9848-4.03940.27962330.188445874820
4.0394-4.09710.24892280.175645824810
4.0971-4.15820.24982410.178145964837
4.1582-4.22310.24032490.169745544803
4.2231-4.29230.2222310.162845944825
4.2923-4.36630.20842450.14945934838
4.3663-4.44560.19732560.141245444800
4.4456-4.53110.19992090.132346374846
4.5311-4.62350.20282300.13146204850
4.6235-4.7240.20032430.128346034846
4.724-4.83370.17432440.121145784822
4.8337-4.95450.18912110.115546334844
4.9545-5.08830.17742380.115146214859
5.0883-5.23790.1682420.118346074849
5.2379-5.40680.1822650.119845834848
5.4068-5.59970.17882290.116246474876
5.5997-5.82360.16632470.109146284875
5.8236-6.08820.17422640.116446144878
6.0882-6.40850.18762390.119246504889
6.4085-6.8090.17592320.116346744906
6.809-7.33320.16222430.108146854928
7.3332-8.06820.17332490.115746824931
8.0682-9.22880.17792750.126846904965
9.2288-11.60170.18422640.139447515015
11.6017-48.91330.2322740.218749235197
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.60190.1776-0.2160.8309-0.12830.62080.0011-0.0676-0.05580.30790.11570.16230.22870.1412-0.11850.83290.1934-0.00520.752-0.08120.9218143.436783.2248-12.4064
22.2674-0.19850.58250.44490.02370.7190.0461-0.94570.44750.3642-0.0338-0.1021-0.0957-0.0237-0.03481.0742-0.23730.08171.8893-0.66281.4875222.4452128.96399.869
32.11061.0036-0.8150.9792-0.56230.2884-0.3449-0.1949-0.7709-0.27740.284-0.75330.36050.21020.04481.24220.2106-0.05581.2213-0.19391.2303164.948270.0686-25.4435
44.9746-1.9673-3.77546.33574.54734.55170.86171.7361.1549-0.4235-0.70341.1084-2.7711-2.5379-0.04242.84350.1202-0.23122.74070.52972.284181.9276132.0355-40.422
54.74052.43922.41439.0562-4.8546.0186-0.98290.58581.0741-0.22570.3979-0.3966-1.06120.03690.53962.501-0.19920.36341.07250.10281.925157.0741156.8048-35.9219
64.58120.11723.45218.32763.74375.69090.2748-0.21651.3885-0.88270.24860.7781-0.847-0.7355-0.40461.5947-0.08990.6090.96960.06832.0965150.46155.7175-27.1376
78.05741.0749-2.15197.9904-2.46576.89440.10720.69821.2275-0.2535-0.4425-0.8058-1.00371.7940.27161.2527-0.53620.55221.7883-0.2061.6723167.1042161.8879-18.6687
81.16952.207-1.1314.6012-1.64031.6520.5221-0.7370.85430.44430.54680.01-0.90661.0842-0.76730.9201-0.42330.33321.4161-0.71431.9448174.1283156.5852-6.3872
98.06012.22290.90898.23060.33483.3178-0.0206-0.0231.53821.60061.0427-1.357-0.49682.1508-0.93241.9084-0.52030.04792.4253-0.72962.3824190.1912161.3837-3.8258
103.0746-0.26521.62766.23290.02972.67960.07670.19920.0673-1.2320.4832-0.5997-1.2290.2534-0.50741.7038-0.17280.77140.9738-0.31791.9583160.3316155.8552-18.1814
110.4270.49520.31972.8285-0.08090.33720.0476-0.1240.37940.50390.0410.5713-0.6668-0.0905-0.08873.122-0.43760.55770.8846-0.26083.0385163.3816174.1589-23.007
120.0952-0.25420.07590.5232-0.16090.04320.2147-0.90390.22060.8213-0.24810.3864-0.0855-0.2940.20381.6786-0.09560.07223.522-1.3441.4306204.7634140.360234.2511
131.4297-1.645-0.49493.02890.25631.573-0.1405-1.39910.46860.70720.17050.73530.3159-0.77130.02021.2147-0.29250.27383.4406-0.97061.7123189.9108131.932918.322
141.1417-1.3576-1.10911.66511.32431.08740.0244-0.54390.00270.56880.2927-0.21510.18910.0513-0.24572.7801-0.4678-0.20721.8379-0.47861.5056153.7696110.878137.901
154.4365-0.9091-0.17362.6436-0.33650.05990.1009-1.24411.17380.8587-0.0676-0.1374-0.75550.58210.04052.0280.03490.1711.457-0.53411.2974160.0004112.58325.6354
164.58491.07071.00244.7393-3.37386.77650.8164-0.3890.1861.12-0.0527-0.01430.5630.0984-0.72.12830.22020.38510.9494-0.1941.0614135.874103.499231.0925
170.5834-0.56180.65110.9648-1.03481.1220.1875-1.07150.23320.7890.04340.09450.5397-0.5687-0.04953.34250.04460.4461.7179-0.32620.8968132.1723109.786647.6304
188.6840.4435-7.84937.8787-0.7919.10941.08780.84421.8756-0.06460.0699-0.4491-1.7579-0.7472-1.18561.47550.055-0.03060.99770.25921.0911139.4349113.76123.4472
194.6804-3.199-4.84536.58615.69986.327-0.3068-1.96861.84811.03860.5019-0.1311-0.25680.427-0.25010.9743-0.08460.14142.2751-0.85691.5541161.6098126.10058.5491
204.1328-0.6343-5.9640.25150.91578.6809-0.0581-0.3510.05990.00940.202-0.4379-0.26090.5402-0.16530.9924-0.18150.16072.6455-1.09491.6349183.708121.3363-2.1319
215.57360.9399-2.3725.25270.70433.86220.2844-0.43960.69530.43560.2572-0.21950.05280.6916-0.37010.873-0.21270.04171.5062-0.88631.5239161.5794128.80534.844
224.5466-5.01294.00648.0796-1.97596.1827-0.6833-0.39452.52441.01550.511-0.9032-1.16111.09260.16761.3651-0.33920.08161.2504-0.56181.7411167.5566132.69655.6924
236.71465.52871.11958.2691-0.16.57380.05930.19951.45910.3828-0.00971.2235-0.5004-0.4602-0.05870.68560.2444-0.00570.9814-0.22911.5412145.1725128.88060.6231
245.40880.4-4.75212.00570.77064.9138-0.48683.3897-2.2035-0.8887-0.31750.89611.0955-1.30720.75650.83320.07830.12111.1219-0.29271.7928152.4048119.6311-10.5377
253.6081-0.2031-3.78193.7491-1.63525.024-0.7374-0.0845-0.52850.26370.2897-0.15520.0005-0.8070.51220.71290.07170.26110.8629-0.33321.4607145.2887116.28311.1952
268.5431-0.48860.84216.73872.99133.56710.0037-0.72740.30860.202-0.0377-0.14050.10410.87210.04140.4748-0.12750.08141.3137-0.32050.9179153.0373120.2088-0.1968
273.2235-4.81041.58737.353-1.93992.1585-0.5195-1.02771.93621.01090.14421.0703-1.7739-1.51370.34311.10620.34460.19351.2015-0.27651.402136.2135133.371-9.1262
285.27890.1765-4.35034.56590.51365.4595-0.03590.581-0.3487-1.46290.09940.48410.23340.12690.01391.24420.0341-0.21412.7947-0.10410.6888173.663114.4686-93.3818
297.33043.03534.83915.37380.59065.51470.09371.1342-0.2603-0.5210.1044-0.5880.14380.6535-0.19170.95810.19990.19941.5302-0.02471.1645174.6871115.4863-87.0482
306.61385.04954.39084.43093.32593.0226-1.24340.4562.3837-1.93890.05622.7351-1.6722-0.66531.15021.74270.1831-0.15141.51960.67951.6066165.5612131.3852-73.9662
319.85267.42050.40238.9613-0.19052.4022-0.39060.17133.11810.3537-0.57421.5631-1.0005-0.14180.83251.3091-0.3447-0.04231.8365-0.54472.6083217.0072143.1018-22.469
328.23273.90251.39811.85630.65120.2561-0.02560.5431.4276-0.38350.20290.2242-0.4386-0.1454-0.14961.6422-0.30220.25161.34090.47363.6865238.8525153.1991-23.9699
331.6476-0.52413.4056.8951-2.14847.2112-0.67890.46270.40950.20450.3107-0.5197-0.23581.10810.35770.87810.08180.44631.5194-0.21522.0812244.6854143.7634-22.2224
341.42562.6779-0.29427.0146-1.98645.45410.07230.07681.7011-0.0408-0.54890.3337-0.55420.16480.42971.4611-0.92010.36731.95440.16762.877252.9196148.4534-36.4948
356.18295.02993.63716.22963.17257.3355-0.02020.833-0.3529-0.97210.2309-0.4574-0.98390.0012-0.18261.1110.25070.37531.88830.07041.6392232.9529137.7344-37.0047
365.1472-0.21053.83420.62260.04937.2332-0.0196-0.0249-0.0489-0.78980.64031.88950.8549-0.0504-0.5881.09780.3542-0.05022.00760.61832.07219.3162125.7872-38.5714
378.49542.06893.06052.05652.95485.90360.3317-0.16830.37780.3607-0.0323-0.5399-0.36510.339-0.28321.0157-0.3013-0.15221.3754-0.42282.1706234.6807136.5902-30.3935
383.3392-2.28962.30656.7718-1.68965.76210.43021.51040.4157-0.9313-0.1491-1.38950.25081.9764-0.2540.97960.03630.4321.9702-0.14881.5845250.2574135.4798-29.9938
396.6677-2.90866.54424.42690.12999.247-0.42751.7366-0.4819-0.7629-0.1106-1.08340.54030.58330.42471.4543-0.11390.12462.4982-0.08141.6774237.4509129.7994-40.1707
405.24810.78184.32123.37580.16683.76820.4143-0.49231.41030.7677-0.30131.851-1.088-1.4575-0.0861.60960.27030.61762.111-0.15472.5154219.0976126.2245-44.7549
419.24971.24920.5551.6929-0.91250.67450.32320.0906-0.2493-0.50890.1044-0.3867-0.11220.72-0.26950.5930.13190.5430.9297-0.04481.2614139.8374122.5324-30.4563
428.73247.55728.26456.64427.0078.70510.0873-0.271.1336-0.6634-0.14130.51-0.69890.10270.09270.860.34470.34230.85810.08861.2039139.0785132.173-31.5891
434.87184.67174.81526.9715.72636.65230.06-0.77230.2738-0.1294-0.661.1085-0.3943-2.36920.64260.44740.15030.07821.2060.31531.6935126.3529125.662-27.5061
445.5008-1.3899-2.41453.54442.37332.063-0.15280.26260.78810.40210.29811.1075-1.6183-1.0501-0.14761.3540.18610.21620.79670.20691.5927130.1849132.883-29.8761
457.79986.32836.76175.15345.48615.8629-0.04010.7241.1098-1.39540.444-0.1255-1.7140.8064-0.44261.22270.05910.43990.93070.15611.3836133.3418131.7121-38.6115
466.6391-2.03453.60449.4009-1.4256.18660.3879-0.72431.33760.8340.69660.0533-0.256-0.4924-0.98230.7552-0.18420.43290.9444-0.16371.5142145.2859139.8599-16.7801
478.28126.88694.27725.78723.79593.12070.5593-0.0635-0.13690.4544-0.2592-0.3354-0.09880.145-0.22530.6158-0.02120.05080.7711-0.04031.2745143.095129.0483-18.5774
481.75-0.69752.06324.92964.45498.71260.00770.4790.2569-0.01010.0422-0.16950.18420.583-0.0630.94760.10530.48020.72940.28451.0246133.5994108.6597-24.4591
495.9672-1.32886.08153.3224-2.97937.0705-0.6146-0.4927-2.29880.74330.52480.66081.69671.37080.09820.90380.45320.54621.0530.38141.974125.3038109.4823-12.2543
509.52772.50516.09232.76991.57163.9311.1061-1.2098-1.19021.6799-0.9573-1.80332.0151-1.1745-0.13941.3504-0.21260.11181.23620.1941.4633133.7666118.9983-8.7782
519.49814.69037.16937.30846.17049.59040.15510.8795-0.46620.35810.2344-0.1532-0.30010.0303-0.4370.85170.03670.3180.7387-0.06081.1391126.0136118.5167-18.9693
525.25134.737-2.11784.3732-1.50042.56250.0452-0.53011.61280.3146-0.1312-0.2546-1.1789-0.83320.0490.69690.21360.17841.03590.08252.2864122.1325119.7454-15.5249
533.60840.6294-2.62749.64650.85562.09780.5073-0.4169-0.90920.3061-0.3391-0.34370.1470.1102-0.21420.75670.05610.06640.81780.50811.0716130.9061117.0609-18.978
543.00020.1792-0.77815.47411.74822.41540.335-0.52290.9544-0.0796-0.07990.0035-0.98920.36-0.17991.527-0.3694-0.06971.5526-0.63512.98238.5689167.0228-3.3823
550.4840.35951.24130.52650.08045.89810.05390.0828-0.48880.0782-0.142-0.7501-0.04440.92020.06191.1253-0.5712-0.31262.7657-0.57953.0023248.79162.2212-2.6452
560.89281.5990.86542.90381.27343.2255-0.6822-0.04120.8276-0.4953-0.49830.1949-0.72110.67571.05371.3332-0.70680.0832.3739-0.29282.8363244.1807159.182-12.2876
571.7692-0.47780.36913.88530.83912.24760.65980.12081.05551.1521-0.1885-0.4665-0.86560.7712-0.3841.619-0.53940.11491.4691-0.55043.2529238.8385165.1121-7.1248
580.7281-1.1133-1.04082.08372.27514.52690.2011-0.65961.1040.50530.2243-0.609-0.66570.8744-0.38222.3707-0.0868-0.33881.1851-0.41592.8326228.9065172.5751-15.6744
590.9974-1.70670.99723.3368-0.94523.48090.3371-0.61231.3841.0235-0.6741-0.44-0.87270.37140.2441.3244-0.28890.13651.8392-0.66932.1798225.4081147.4672-5.4847
606.679-3.62663.0532.3355-3.32669.00060.7616-0.3023-0.50330.4646-0.02310.21611.4843-0.6155-0.70461.2602-0.3792-0.00032.1711-0.96931.3749222.2972124.3468-6.8311
615.4193.12634.5951.88522.4634.2857-0.48250.04420.83270.1898-0.28810.4598-0.8335-0.54110.71461.4903-0.06830.262.1735-1.30022.9584220.9612161.36728.4156
620.42371.17111.07253.33943.14853.0461-0.287-0.70771.35531.78530.8155-1.3136-2.73060.6731-0.61282.9227-0.2274-0.01112.2077-1.49883.0515227.0176165.31124.0579
633.7166-4.26681.30258.4455-2.34678.4082-0.1791-0.08580.8450.36090.1903-0.2428-0.44990.9327-0.0391.265-0.530.25682.7077-0.85681.5813224.8307141.628218.0136
641.4001-0.23771.07290.3769-0.67231.9317-0.245-0.92150.8140.17560.08580.2228-0.3870.00590.12041.3058-0.37610.4112.3117-0.82221.5557218.0016134.92513.6878
655.16140.5712.87874.19470.27424.55160.6713-1.51771.03952.927-1.07231.2208-0.7275-0.11230.47692.4753-0.4494-0.40672.3676-1.50763.0955229.8975168.431232.5297
660.6161-0.94190.40551.4872-0.56760.32110.0667-1.50811.29021.14160.25940.7772-1.07390.1237-0.41542.24720.34970.53022.2678-1.29152.992222.9365171.086240.4968
675.165-6.6127-1.58988.60161.98510.65230.45320.20180.5149-0.1834-0.0733-0.0985-1.08511.4199-0.36412.32380.00180.37913.2052-0.81222.0044215.2496157.924536.3597
685.9568-1.9645-0.2475.8853-0.23812.0790.9161-1.80120.91761.5991-0.4113-1.5415-1.05221.6156-0.47322.05520.064-0.57332.9263-1.20222.2175226.6188155.25134.4692
696.302-1.74845.71117.2549-3.05956.0351-0.27440.36210.0015-0.16630.2697-0.076-0.7029-0.4474-0.11580.64540.03290.25781.20570.03371.3825212.829118.6464-69.4171
709.7729-1.65937.72015.7611-0.38029.0018-0.06380.11510.238-0.10370.2565-0.5984-0.14130.439-0.1960.8931-0.07870.22611.30210.05770.9263216.9064114.9514-62.7124
716.72754.2421-5.4256.6367-5.46515.42550.13250.2610.3496-0.0715-0.5039-1.3450.04751.02110.32110.50490.13780.10041.5999-0.30131.1909220.9189122.0268-64.5595
729.51153.95420.0998.8447-2.18846.7387-0.3808-0.64450.55850.4518-0.56770.96050.1249-0.75150.79220.7326-0.10970.36991.5295-0.34421.1561211.0348125.8319-64.8744
733.9657-1.17834.39216.65363.08677.9215-0.2750.15240.2946-0.3301-0.8301-1.4355-1.00361.63781.11141.0293-0.21440.17211.42460.27271.3759210.581129.7826-69.8993
741.744-1.43272.71015.4555-2.85654.3910.2830.6364-0.05520.0248-0.0328-0.21440.13750.3267-0.17691.1390.0310.49091.7199-0.42821.7337193.3365110.2245-57.765
754.0628-2.86175.28062.0158-3.72536.83150.58220.0058-0.2593-0.34240.39840.11861.15320.6752-0.84160.99550.4619-0.19741.40690.07641.549153.020788.8157-13.5475
764.80530.7325-2.61263.9372-0.64711.51490.1721-0.0461-0.54150.66660.1765-0.88920.33261.0529-0.25861.13910.2658-0.6231.4523-0.23461.4799173.581578.73310.4929
778.5629-3.22791.24922.49031.45083.3307-0.4194-0.4696-0.74550.04980.24061.16610.6327-0.13850.02491.4502-0.140.22861.8481-0.22182.3918273.4769116.4356-8.0721
782.9778-2.64281.86312.35-1.7051.7104-0.0626-0.06511.0440.05020.0829-0.47420.08490.6027-0.00741.41850.7323-0.58331.8177-0.20972.4122268.5783120.8296-5.2521
797.06262.6773-0.7964.0796-2.9294.9560.3617-0.20290.92790.0484-0.6686-1.0764-0.01221.14610.37421.12190.29190.08362.073-0.35542.2871275.6073122.6055-0.9788
803.78114.588-2.33525.5572-3.05986.190.4965-2.2114-0.16961.3802-0.762-0.801-0.19970.63490.19741.2879-0.20140.05062.1606-0.00211.2383265.2834104.80228.7554
815.2427-0.73062.09777.6489-5.18884.0147-0.1114-2.35590.20251.92310.0043-0.204-0.0852-0.07530.09861.85020.299-0.09493.0901-0.34181.539249.624898.909813.7903
827.62062.61033.12253.57580.44578.5728-0.2659-0.1965-1.40430.02240.14980.90210.23320.59850.17081.08090.0640.11651.712-0.1741.7653240.6557103.62842.3262
837.6509-2.2857-2.48053.9335-3.81617.18970.2227-0.44090.1380.6474-0.0642-0.8405-0.19830.0079-0.09811.4906-0.3493-0.07133.5222-0.69471.4848220.0544116.775234.2262
843.78740.7223-2.11513.50692.68484.5878-2.6154-6.47541.93675.25273.37211.70.26851.9253-0.77411.80970.40380.27423.1141-0.83632.0554231.3389118.550339.5063
850.6593-0.149-0.83535.49140.70261.64160.5771-0.775-0.20190.5281-0.2466-0.2592-0.28380.4794-0.32350.9914-0.3590.06582.4594-0.46591.7988227.9095120.766930.6789
867.9887-4.7132-5.66072.91463.30314.1579-0.2718-0.52170.14630.92970.5615-0.72180.65041.688-0.33961.29040.0055-0.16122.004-0.29511.5662215.064124.19727.0773
872.09490.01460.20772.6746-0.91787.3052-0.1741-1.14771.27550.3692-0.45150.15720.3092-0.27120.51791.19840.0271-0.14832.607-0.96581.7461216.272133.820620.5307
881.8208-2.1173-0.34378.1698-2.9069.4536-0.19313.84622.1532-2.98920.31660.4218-0.7543-0.0661-0.03691.64070.1928-0.0262.32380.72231.4449141.0382110.6688-74.2216
898.9184-6.4429-0.51229.35661.25182.0884-0.10090.5992-0.7721-0.49710.7560.4293-0.4864-0.338-0.70360.96980.0793-0.20871.41540.02831.0286144.7076104.2108-66.5807
908.71880.25441.97763.5399-0.31773.31490.2658-1.1406-0.24290.01380.43370.31170.30640.7438-0.60950.93010.0205-0.32541.6262-0.17271.1347143.5179101.7863-56.3584
919.21233.2962-7.84657.12231.28099.5272-0.31490.8029-3.18410.2636-0.360.47561.3044-1.23660.55991.0749-0.3618-0.54841.45970.50032.5244131.068497.5494-63.6977
926.3219-1.34831.89690.6624-0.98551.5377-0.1425-0.7187-0.14770.69780.34120.17430.15260.0547-0.20451.60030.13510.66530.77610.01831.4783117.308276.667315.2228
934.0056-0.32222.09221.2751-2.54475.6238-0.3268-0.48161.31590.36870.26530.79760.14680.16480.07821.40840.19880.24060.7837-0.14531.6366114.881577.013410.717
945.3615.91523.40128.53085.07573.2013-0.8766-0.62092.38690.1352-0.05311.4931-3.0944-0.43431.04631.92270.17730.54570.77410.06681.5577109.489686.256820.7855
957.05873.8152-0.73082.2088-0.37071.393-0.1907-0.10581.0198-0.3714-0.0620.4692-1.352-0.3660.23591.68840.41990.56951.13130.39352.1912100.770479.12135.8047
967.78432.80775.83665.24612.38616.53370.5087-1.3992-0.45441.4319-0.25661.28020.0999-0.8778-0.26371.7298-0.2370.81590.87280.07311.8413101.092274.737316.5387
972.46311.21720.95445.29680.72680.382-0.44010.70110.354-0.60950.6842-0.30950.7404-0.6713-0.22640.8318-0.10060.15851.29770.26090.9024119.980.636-38.3152
983.51081.77690.62875.87281.03353.646-0.08680.18640.7253-0.79840.29481.02370.0516-0.2086-0.19490.8402-0.1974-0.15091.10830.30271.1141121.864784.5196-35.4546
997.291-6.12281.23765.4845-2.72118.5579-0.11290.10540.8423-0.08030.5672-0.37790.2713-0.0533-0.41430.9225-0.22480.08361.3597-0.01551.3742137.916685.5366-44.2361
1004.0941-4.6387-0.02395.90881.61643.858-0.2752-2.05664.92880.2184-0.0252-1.5627-3.26331.29320.31651.766-0.1845-0.07081.0784-0.28711.8284180.4623139.8722-64.0047
1017.76520.1426-1.91932.7148-1.36723.2836-0.9135-0.75573.2880.0095-0.19320.654-1.7528-0.40981.09162.1747-0.0791-0.10391.467-0.14962.0545174.7196140.5358-72.0813
1025.8249-1.8813-5.265.63183.06615.1228-0.39860.72621.1081-0.6675-0.44480.4553-0.561-0.63330.75651.28640.4089-0.08491.99040.22051.3681172.3631128.9098-76.8995
1032.00023.7815.16063.2524.576.42430.33691.49821.6462-1.40390.3956-0.8984-1.86681.6225-0.76991.06970.19950.69111.34040.48651.6005181.166132.9665-74.4696
1044.4064-5.4402-1.86176.73362.13322.25510.25570.53140.6868-0.2420.1817-2.9518-0.24862.534-0.4020.71970.1182-0.08512.0673-0.01151.8465186.5748128.2632-66.104
1058.2209-2.817-5.51437.69151.79796.61210.57590.35890.8911-0.22860.37650.3036-0.4595-0.2446-0.86861.15240.10130.26181.45760.29880.7415175.5708126.8657-67.0602
1063.61890.78441.48811.68680.11112.55050.40180.57370.30350.13510.2068-1.22970.08410.6422-0.58551.0540.37850.14231.80570.18181.8539192.7884125.66-70.9515
1073.32152.5331-2.77547.88-6.21146.0285-0.57841.0465-0.6515-1.29560.70880.76482.7029-2.7489-0.1251.4736-0.6098-0.612.4571-0.2172.103252.1043113.258927.757
1080.34870.4388-0.50120.5619-0.6590.79260.0872-1.502-0.75371.2279-0.0213-0.21720.1692-0.644-0.0552.6690.8111-0.69823.41210.02391.74251.773799.127542.1493
1093.99613.2202-1.43164.10510.17553.50870.1802-0.04260.66490.4239-0.2771-1.17190.09540.61410.20382.1667-0.50910.10582.14420.10062.084247.123392.79836.9304
1100.03650.30060.143.77332.56062.05790.0442-0.85980.40470.5887-0.4284-0.3472-0.1214-0.18770.38851.54550.0403-0.55873.9362-0.21231.497247.3948104.592829.3496
1110.91470.5691-2.218.3645-0.73555.37650.2165-1.7221-0.33290.8731-0.0753-1.21450.3841.9511-0.07481.3129-0.0546-0.25412.38920.17761.5467250.391496.312228.8791
1127.8027-1.94875.44143.0083-0.36824.2001-0.1745-0.3067-0.38350.26650.0627-0.30850.45180.72210.12681.8277-0.33970.03651.88140.03612.1077241.813397.03617.8222
1132.6471-0.542.95462.9064-0.38247.3950.25780.0341-0.4325-0.30270.52010.70911.1251-0.3365-0.71461.4975-0.2740.05320.87050.24731.162107.875141.6384-19.8097
1140.01030.00020.00420.00120.00730.06970.0926-1.2905-0.33420.97560.17180.71090.2386-0.2786-0.26431.6631-0.16360.10651.24880.30051.2024105.394251.2814-14.8641
1153.28272.15950.94195.98910.88496.2674-0.33940.81520.7408-0.2930.3885-0.2961-1.31521.0603-0.07491.7967-0.8408-0.12561.43840.40441.7135103.066651.9467-31.391
1163.6918-4.6362-4.2016.99147.39248.87690.0733-0.9062-0.69870.5932-0.97581.62331.1537-0.85620.7651.2296-0.479-0.08061.84560.20171.272992.604947.6852-38.8251
1177.014-4.6831-0.93044.84662.07641.7922-0.0585-0.92260.61441.33890.19140.3075-0.44360.1294-0.07171.5843-0.10120.1092.2633-1.10432.0281251.6252124.8492.9047
1185.2035-4.45673.61453.8327-3.00653.01380.2993-0.53290.5680.0425-0.0208-0.4989-0.0560.456-0.231.7454-0.25520.0891.8851-0.90881.9158247.9136129.76864.4867
1197.34291.57243.34151.7672-0.18197.409-0.0846-0.47180.51641.0519-0.3289-0.1948-0.7153-0.01630.36881.7838-0.5788-0.19223.0441-1.23691.8889253.8495132.802610.924
1200.15910.1146-0.22822.2231-1.59811.2898-0.3828-2.90461.44830.98861.2076-1.75940.20582.7391-0.811.75740.4936-0.4454.2959-0.96093.7711192.871199.6446-2.3023
1214.89395.6812.4247.75380.37676.3421-0.4754-0.90081.22360.6203-0.1734-0.3686-0.91610.2570.52793.16961.4023-0.06444.6018-0.23573.2375203.255395.80033.968
1228.3621.46921.05018.2679-5.28613.866-0.9176-3.15882.70023.58881.6344-0.73820.41311.1846-0.91833.05220.13970.05573.11-0.88712.0846182.2628123.1354-39.4828
1232.05730.2152-0.14213.99030.80850.18930.192-0.47150.05030.5073-0.04070.1039-0.0729-0.2134-0.12571.78860.86190.0763.4132-0.67612.2135203.0202101.9122-20.6743
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resseq 5:920)A5 - 920
2X-RAY DIFFRACTION2chain 'A' and (resseq 921:1400)A921 - 1400
3X-RAY DIFFRACTION3chain 'A' and (resseq 1401:1532)A1401 - 1532
4X-RAY DIFFRACTION4chain 'A' and (resseq 1533:1540)A1533 - 1540
5X-RAY DIFFRACTION5chain 'B' and (resseq 7:25)B7 - 25
6X-RAY DIFFRACTION6chain 'B' and (resseq 26:64)B26 - 64
7X-RAY DIFFRACTION7chain 'B' and (resseq 65:86)B65 - 86
8X-RAY DIFFRACTION8chain 'B' and (resseq 87:122)B87 - 122
9X-RAY DIFFRACTION9chain 'B' and (resseq 123:148)B123 - 148
10X-RAY DIFFRACTION10chain 'B' and (resseq 149:226)B149 - 226
11X-RAY DIFFRACTION11chain 'B' and (resseq 227:240)B227 - 240
12X-RAY DIFFRACTION12chain 'C' and (resseq 2:112)C2 - 112
13X-RAY DIFFRACTION13chain 'C' and (resseq 113:207)C113 - 207
14X-RAY DIFFRACTION14chain 'D' and (resseq 2:34)D2 - 34
15X-RAY DIFFRACTION15chain 'D' and (resseq 35:67)D35 - 67
16X-RAY DIFFRACTION16chain 'D' and (resseq 68:148)D68 - 148
17X-RAY DIFFRACTION17chain 'D' and (resseq 149:181)D149 - 181
18X-RAY DIFFRACTION18chain 'D' and (resseq 182:209)D182 - 209
19X-RAY DIFFRACTION19chain 'E' and (resseq 5:14)E5 - 14
20X-RAY DIFFRACTION20chain 'E' and (resseq 15:27)E15 - 27
21X-RAY DIFFRACTION21chain 'E' and (resseq 28:50)E28 - 50
22X-RAY DIFFRACTION22chain 'E' and (resseq 51:63)E51 - 63
23X-RAY DIFFRACTION23chain 'E' and (resseq 64:79)E64 - 79
24X-RAY DIFFRACTION24chain 'E' and (resseq 80:86)E80 - 86
25X-RAY DIFFRACTION25chain 'E' and (resseq 87:103)E87 - 103
26X-RAY DIFFRACTION26chain 'E' and (resseq 104:140)E104 - 140
27X-RAY DIFFRACTION27chain 'E' and (resseq 141:154)E141 - 154
28X-RAY DIFFRACTION28chain 'F' and (resseq 1:32)F1 - 32
29X-RAY DIFFRACTION29chain 'F' and (resseq 33:92)F33 - 92
30X-RAY DIFFRACTION30chain 'F' and (resseq 93:101)F93 - 101
31X-RAY DIFFRACTION31chain 'G' and (resseq 2:11)G2 - 11
32X-RAY DIFFRACTION32chain 'G' and (resseq 12:20)G12 - 20
33X-RAY DIFFRACTION33chain 'G' and (resseq 21:52)G21 - 52
34X-RAY DIFFRACTION34chain 'G' and (resseq 53:57)G53 - 57
35X-RAY DIFFRACTION35chain 'G' and (resseq 58:79)G58 - 79
36X-RAY DIFFRACTION36chain 'G' and (resseq 80:92)G80 - 92
37X-RAY DIFFRACTION37chain 'G' and (resseq 93:109)G93 - 109
38X-RAY DIFFRACTION38chain 'G' and (resseq 110:129)G110 - 129
39X-RAY DIFFRACTION39chain 'G' and (resseq 130:144)G130 - 144
40X-RAY DIFFRACTION40chain 'G' and (resseq 145:156)G145 - 156
41X-RAY DIFFRACTION41chain 'H' and (resseq 1:18)H1 - 18
42X-RAY DIFFRACTION42chain 'H' and (resseq 19:29)H19 - 29
43X-RAY DIFFRACTION43chain 'H' and (resseq 30:41)H30 - 41
44X-RAY DIFFRACTION44chain 'H' and (resseq 42:53)H42 - 53
45X-RAY DIFFRACTION45chain 'H' and (resseq 54:62)H54 - 62
46X-RAY DIFFRACTION46chain 'H' and (resseq 63:72)H63 - 72
47X-RAY DIFFRACTION47chain 'H' and (resseq 73:85)H73 - 85
48X-RAY DIFFRACTION48chain 'H' and (resseq 86:95)H86 - 95
49X-RAY DIFFRACTION49chain 'H' and (resseq 96:100)H96 - 100
50X-RAY DIFFRACTION50chain 'H' and (resseq 101:106)H101 - 106
51X-RAY DIFFRACTION51chain 'H' and (resseq 107:116)H107 - 116
52X-RAY DIFFRACTION52chain 'H' and (resseq 117:127)H117 - 127
53X-RAY DIFFRACTION53chain 'H' and (resseq 128:138)H128 - 138
54X-RAY DIFFRACTION54chain 'I' and (resseq 2:25)I2 - 25
55X-RAY DIFFRACTION55chain 'I' and (resseq 26:32)I26 - 32
56X-RAY DIFFRACTION56chain 'I' and (resseq 33:53)I33 - 53
57X-RAY DIFFRACTION57chain 'I' and (resseq 54:88)I54 - 88
58X-RAY DIFFRACTION58chain 'I' and (resseq 89:97)I89 - 97
59X-RAY DIFFRACTION59chain 'I' and (resseq 98:117)I98 - 117
60X-RAY DIFFRACTION60chain 'I' and (resseq 118:128)I118 - 128
61X-RAY DIFFRACTION61chain 'J' and (resseq 3:29)J3 - 29
62X-RAY DIFFRACTION62chain 'J' and (resseq 30:42)J30 - 42
63X-RAY DIFFRACTION63chain 'J' and (resseq 43:47)J43 - 47
64X-RAY DIFFRACTION64chain 'J' and (resseq 48:70)J48 - 70
65X-RAY DIFFRACTION65chain 'J' and (resseq 71:78)J71 - 78
66X-RAY DIFFRACTION66chain 'J' and (resseq 79:85)J79 - 85
67X-RAY DIFFRACTION67chain 'J' and (resseq 86:93)J86 - 93
68X-RAY DIFFRACTION68chain 'J' and (resseq 94:100)J94 - 100
69X-RAY DIFFRACTION69chain 'K' and (resseq 11:34)K11 - 34
70X-RAY DIFFRACTION70chain 'K' and (resseq 35:57)K35 - 57
71X-RAY DIFFRACTION71chain 'K' and (resseq 58:75)K58 - 75
72X-RAY DIFFRACTION72chain 'K' and (resseq 76:100)K76 - 100
73X-RAY DIFFRACTION73chain 'K' and (resseq 101:110)K101 - 110
74X-RAY DIFFRACTION74chain 'K' and (resseq 111:126)K111 - 126
75X-RAY DIFFRACTION75chain 'L' and (resseq 5:32)L5 - 32
76X-RAY DIFFRACTION76chain 'L' and (resseq 33:128)L33 - 128
77X-RAY DIFFRACTION77chain 'M' and (resseq 2:14)M2 - 14
78X-RAY DIFFRACTION78chain 'M' and (resseq 15:21)M15 - 21
79X-RAY DIFFRACTION79chain 'M' and (resseq 22:61)M22 - 61
80X-RAY DIFFRACTION80chain 'M' and (resseq 62:83)M62 - 83
81X-RAY DIFFRACTION81chain 'M' and (resseq 84:93)M84 - 93
82X-RAY DIFFRACTION82chain 'M' and (resseq 94:119)M94 - 119
83X-RAY DIFFRACTION83chain 'N' and (resseq 2:11)N2 - 11
84X-RAY DIFFRACTION84chain 'N' and (resseq 12:16)N12 - 16
85X-RAY DIFFRACTION85chain 'N' and (resseq 17:24)N17 - 24
86X-RAY DIFFRACTION86chain 'N' and (resseq 25:29)N25 - 29
87X-RAY DIFFRACTION87chain 'N' and (resseq 30:61)N30 - 61
88X-RAY DIFFRACTION88chain 'O' and (resseq 2:15)O2 - 15
89X-RAY DIFFRACTION89chain 'O' and (resseq 16:49)O16 - 49
90X-RAY DIFFRACTION90chain 'O' and (resseq 50:73)O50 - 73
91X-RAY DIFFRACTION91chain 'O' and (resseq 74:88)O74 - 88
92X-RAY DIFFRACTION92chain 'P' and (resseq 1:19)P1 - 19
93X-RAY DIFFRACTION93chain 'P' and (resseq 20:43)P20 - 43
94X-RAY DIFFRACTION94chain 'P' and (resseq 44:52)P44 - 52
95X-RAY DIFFRACTION95chain 'P' and (resseq 53:62)P53 - 62
96X-RAY DIFFRACTION96chain 'P' and (resseq 63:83)P63 - 83
97X-RAY DIFFRACTION97chain 'Q' and (resseq 2:17)Q2 - 17
98X-RAY DIFFRACTION98chain 'Q' and (resseq 18:82)Q18 - 82
99X-RAY DIFFRACTION99chain 'Q' and (resseq 83:100)Q83 - 100
100X-RAY DIFFRACTION100chain 'R' and (resseq 19:23)R19 - 23
101X-RAY DIFFRACTION101chain 'R' and (resseq 24:28)R24 - 28
102X-RAY DIFFRACTION102chain 'R' and (resseq 29:36)R29 - 36
103X-RAY DIFFRACTION103chain 'R' and (resseq 37:43)R37 - 43
104X-RAY DIFFRACTION104chain 'R' and (resseq 44:52)R44 - 52
105X-RAY DIFFRACTION105chain 'R' and (resseq 53:76)R53 - 76
106X-RAY DIFFRACTION106chain 'R' and (resseq 77:88)R77 - 88
107X-RAY DIFFRACTION107chain 'S' and (resseq 2:12)S2 - 12
108X-RAY DIFFRACTION108chain 'S' and (resseq 13:25)S13 - 25
109X-RAY DIFFRACTION109chain 'S' and (resseq 26:35)S26 - 35
110X-RAY DIFFRACTION110chain 'S' and (resseq 36:40)S36 - 40
111X-RAY DIFFRACTION111chain 'S' and (resseq 41:70)S41 - 70
112X-RAY DIFFRACTION112chain 'S' and (resseq 71:81)S71 - 81
113X-RAY DIFFRACTION113chain 'T' and (resseq 8:66)T8 - 66
114X-RAY DIFFRACTION114chain 'T' and (resseq 67:75)T67 - 75
115X-RAY DIFFRACTION115chain 'T' and (resseq 76:91)T76 - 91
116X-RAY DIFFRACTION116chain 'T' and (resseq 92:106)T92 - 106
117X-RAY DIFFRACTION117chain 'U' and (resseq 2:8)U2 - 8
118X-RAY DIFFRACTION118chain 'U' and (resseq 9:15)U9 - 15
119X-RAY DIFFRACTION119chain 'U' and (resseq 16:25)U16 - 25
120X-RAY DIFFRACTION120chain 'V' and (resseq 1:4)V1 - 4
121X-RAY DIFFRACTION121chain 'W' and (resseq 32:40)W32 - 40
122X-RAY DIFFRACTION122chain 'a' and (resseq 33:40)a33 - 40
123X-RAY DIFFRACTION123chain 'b' and (resseq 1:2)b1 - 2

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